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We narrowed to 836 results for: RING

Showing: 321 - 340 of 836 results

  1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...then used to measure the frequency of each variant during the selection process. Each variant is assigned...of MnCl2. Disadvantages of this method include favoring of certain types of mutations, difficulty controlling...then both strands of the plasmid will be nicked during the first BbvCI digest and the whole plasmid will...direction, both strands of the plasmid will be nicked during the first BbvCI digest and the whole plasmid will...Douglas M., Jason J. Stephany, and Stanley Fields. “Measuring the Activity of Protein Variants on a Large Scale...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...unfamiliar, scFvs are often the building blocks for engineering proteins. So let’s get acquainted with scFvs....CD3 on T cells and a tumor-specific antigen, it brings T cells to a tumor site. ScFvs can also be fused...viral vectors like AAV, which could be useful for delivering scFvs that inhibit HIV.   References and Resources...Bruccoleri RE, Haber E, Crea R (1988) Protein engineering of antibody binding sites: recovery of specific...more about antibodies  Read about another reagent sharing platform for antibodies, the Developmental Studies...

  3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...Proximity labelling relies on the labelling of neighboring proteins within a cell with biotin. Biotin, is...promiscuous biotin ligase that can efficiently label neighboring proteins The first biotin based proximity labelling...from the ligase and bind to exposed lysines on neighboring proteins. Thus to capture protein complexes, ...electron-rich amino acids such as tyrosine on neighboring proteins resulting in their biotinylation. The...The advantage of APEX is its speed, labeling neighboring proteins in minutes rather than hours like BioID...

  4. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ... nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. What you ...4740. You can find more info on pCri in Mary Gearing’s blog post on the system Easing the Protein Purification...Seq kit has been used in microbiology studies monitoring sequence dynamics under various selective pressures... can lead to surprisingly large fitness effects during adaptive evolution of antibiotic resistance." Proceedings...2016): 895-899. Choudhury, Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...post-translational modifications, identifies a staggering ~250,000 phosphorylation events in the proteomes... led to the eventual discovery of a naturally occurring phosphoserine-tRNA synthetase that serves as an...elongation factor that accepts Sep-charged tRNAs during translation. Many ‘flavors’ of Sep-OTS plasmids...pSer-containing GFP that can be used as a positive control during optimization experiments. This version of GFP will...dephosphorylate the pSer-incorporated protein, ideally bringing the phenotype assayed closer to that of the wild...

  6. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...How much do you need [for your lab start up]?” A hiring chair or dean will often ask a faculty candidate...community is the ultimate value proposition. By preparing a budget before hand, you’re saving the institute...turned down a faculty position because none of the offering institutes were able to provide the resources ...If the resources aren’t available, then you’re entering a win-lose situation that can ultimately set you... be afraid to walk away from a negotiation. If securing a faculty position is your goal, then a resource...

  7. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ..., and as of today, will stop posting, monitoring, and resharing content on X.  Addgene deeply values our..., TikTok, Facebook, and LinkedIn. We are also exploring other social media platforms with active scientific...in the scientific community. Addgene has been considering a gradual move off X as a response to major platform...

  8. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...the recent Keystone Meeting "Precision Genome Engineering and Synthetic Biology". His reflections on the...scientific community. Data on CRISPR and synBio plasmid sharing In a recent review on CRISPR technology, for example...online user forums such as http://www.genome-engineering.org and http://www.egenome.org." At the Keystone...Keystone Meeting Eric presented a poster with data comparing Addgene global deposit and distribution trends...

  9. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...accosted by dozens of tiny black dots during our stay there. Yet capturing the flies is nearly impossible, ..., due to their small size and inconspicuous coloring.  FLIS plasmid Recently, the Phuels lab deposited...everywhere find and re-contain any specimens that escape during routine lab activities.  Here, we demonstrate the...

  10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger nucleases...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...Sauka-Spengler T (2017). Genome and epigenome engineering CRISPR toolkit for probing in vivo cis-regulatory... Mary Gearing...

  11. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...treated identically, used to assess naturally occurring random biological variability. If one were studying...species-on-species staining can be partially addressed with stringent staining protocols and the use of isotype-specific...standard protocol, but the primary antibody is omitted during the incubation step. Samples are incubated with...will briefly touch on.  Western blotting When comparing samples in different lanes in a western blot, ...controls address the spectral overlap that occurs during multiplexing. Compensation controls are single ...

  12. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...cell-type specific. If it is something you’re considering for your experiment, note that you cannot extrapolate...hardware types, construction, and testing used for delivering your light stimulus. This can make or break your...Photoswitching Proteins (Vol. 2173, pp. 21–51). Springer US. https://doi.org/10.1007/978-1-0716-0755-8_...2019). Cortical layer–specific critical dynamics triggering perception. Science, 365(6453), eaaw5202. https...

  13. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...bound repressor then prevents transcription from occurring, which means that transcription is now OFF.   ... ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase of yeast culture... al. Using Binary Systems Together You may be wondering, “why is it necessary to have all 3 of the different...experiments! This post was originally written by Mary Gearing in 2018, and updated by Hannah Dotson in Dec. 2022...Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS...

  14. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...CRISPR during his postdoc 12:01 - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why...the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had on the podcast... of medical school. 7:05 - 10:55 : Alex's focus during graduate school - DNA damage and longevity. 10:...

  15. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Series. We’ll be covering the basics of science communication, tips and tricks for sharing your science with...Addgene TikTok at the end of this month! We’ll be sharing our educational resources and tips and tricks for... now! (Bonus: Tim kindly offered to finishing answering all submitted questions via blog post, which we...

  16. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - Developing Addgene’s ...Future, the research team, and the importance of sharing information/knowledge Outro Many thanks to our...received his Bachelor of Science degree in Computer Engineering from Tufts University and his Master of Arts ...Bioworks. He also holds a SB and a Masters in Engineering from MIT, both in Computer Science.   Special...

  17. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...I can bring to Addgene -- that comfort with saying, “if we do this, then what?” I hope to bring that business...my personality. Another big part of my role is bringing experience and judgement to a wide variety of ...business world from the mindset of a lawyer can bring a new perspective to an organization. I'm used to...

  18. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ... grown steadily during 2014. We were excited to have more and more scientists offering to share their ...Will You Be My Mentor? Finding and Asking for Mentoring Support By Joanne Kamens Plasmids 101: Antibiotic...Margo R. Monroe Best wishes for your research during this holiday season and we look forward to seeing...

  19. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...are thought to interrogate dsDNA for proper base pairing through minor grove contacts, and thus do not read... do with the slight melting of the 5 ́-terminus during the reaction. This “peeling back” of the 5 ́-phosphorylated...upstream probes and four down-stream probes, each differing only in the base at the ligation junction. Thus...prepared, each with a different complement strand covering all 16 possible NN pairs in the template. The ...The probes were designed such that each possible pairing resulted in a product of a different length, with...

  20. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...protein can also influence antibody binding by altering the relative concentration of potential antibody...to validate that your antibody is specifically capturing your RNA-binding protein of interest. So you ...antibody’s trustworthiness. You may also consider sharing your validation attempts by other means, such as.... These outlets can be particularly useful for sharing negative data. Negative data often goes overlooked...antibody (perhaps one from Addgene!), consider sharing your results so that others in the community can...
Showing: 321 - 340 of 836 results