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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 517 results for: SET

Showing: 321 - 340 of 517 results

  2. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    .... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...independent variable. Biological replicates In any given set of experiments, the biological controls are more ...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

  11. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...gene-targeting capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene...genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777...

  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...your “gating strategy.” This series of markers are set up sequentially, from broadest to most specific marker...Subtypes of immune cells are often defined by a subset of the markers used to identify them, such as “...

  14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...it’s a combination of many factors unique to this set of tools and the community they serve. As difficult...validating and using an AAV in a specific experimental setup. Teams across Addgene have worked hard to ensure...compared to other AAVs preps from 2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and...

  15. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ... document examples are not especially helpful. I set out to make something practical and accessible. If...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...Tempurature: Analyze your expression conditions by setting up a small-scale expression experiment to test ...StrR, TetR) Expression of insoluble proteins  Rosetta2 (DE3)* Chloramphenicol (pRARE) Good for “universal... plasmid contains a chloramphenicol resistance cassette for positive selection and a p15A origin of replication...

  20. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...PMCID: PMC3748948.  Khetrapal, Varnica, et al. "A set of powerful negative selection systems for unmodified...expression of a gene with an antibiotic resistance cassette. This leaves a “scar” in the chromosome, sometimes...vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of replication to allow replication...pDS132 (Philippe et al., 2004): Replaced the sacB cassette with one of three toxins from the Khetrapal paper...medicine and clinical infectious diseases at the Massachusetts General Hospital where he is now an attending...
Showing: 321 - 340 of 517 results