We narrowed to 852 results for: STI;

...synthesis (those of us who have used antibodies for diagnostics know how frustrating it can be to get a “bad ...the bound protein fluorophores and quenchers are still relatively bulky. One of the advantages of oligonucleotides...stable and brighter version of the aptamer, but it still had limitations in vivo. Using a combination of ... a lower magnesium dependence. Though initially still unstable in eukaryotes, the Jaffrey lab has since...et al. “Aptamer-based assay for prion diseases diagnostic”. Procedia Environmental Sciences. Volume 12,...

... during the early stages of the process. Some questions to start with are: Is your sequence of interest...opposite (reverse) orientation? The answers to questions like these will determine which acceptor vectors...assembly efficiency. This can be done through domestication, which is the modification of a DNA sequence... the synonymous mutation of a codon. You can domesticate the sites yourself using PCR, but gene synthesis...gene synthesis, you can request the sites to be domesticated through the process, and request your sequence...

... plasmid is delivered to your target cells, you still need to select for the cells that contain it. Antibiotic... approach in a mammalian cell experiment should still include an antibiotic resistance gene for growth...express your protein of interest. However, you do still need a species-compatible promoter for expressing... consider the viral vectors themselves to be a distinct gene delivery method. We focused this post on ...between prokaryotes and eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak...

...was applying. I probably did not respond to her question as well then as I would now. I would have to repress... repress laughter if I was asked that question today, because I sometimes wish for a stretch of time that...me, “I find that at a desk, it is much easier to stick to timelines. This is probably because you don't...careers and desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available ...into science in the first place. Finally, if you still find the idea of not holding a pipette again upsetting...

...the way we apprehend molecular complexes and is still a very useful tool.    Like a guitar hero (that ...SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs to measure FRET... is difficult to address especially when you investigate unknown molecular complexes. But it is a parameter... faster measurement systems, this technique has still many good days and good “gigs” ahead...No offense...Chemiluminescence Resonance Energy Transfer    Interesting Articles About FRET: Piston DW, Kremers GJ. ...

...GFP-like probes by supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional...mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological Studies) Multiple uses: From...applications.  There is a whole set of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names...occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For...developed based on mIFP6. Learn more about the characteristics, performance, and applications of near-infrared...

...epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive lasting transcriptional...cofactor fused to a histone tail to recruit and stimulate the endogenous methyltransferase. The strategy...H3K4me0 on D3L, releasing the autoinhibition and stimulating the methylation of nearby CpG sites on the target...expression off. Plus, DNA methylation is a long-lasting repressive marker, allowing the team to develop...

...potential for therapeutic drug discovery as it is estimated that 75% of disease causing genes in humans have...Stainer group determined that there are actually two distinct populations of β-cells that originate in different...other model organisms. Worms have been used to investigate an array of biological systems and processes ...PubMed PMID: 9486653. Hesselson, Daniel, et al. "Distinct populations of quiescent and proliferative pancreatic...19706417. PubMed Central PMCID: PMC2736433. Howe, Kerstin, et al. "The zebrafish reference genome sequence...

...article is to provide enough background for you to distinguish the features of any common lab strain and determine...them in 1885. E. coli are mainly found in the intestinal tract of animals. There are many different naturally...hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated... Hte   High transformation efficiency deoR constitutive expression of genes for deoxyribose synthesis...strains; however, these tables are by no means exhaustive! For a more comprehensive list and additional...

...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...downstream characterization of, isolating 2 or 3 distinct clones to perform key experiments on is a good...principle holds true - the higher the number of distinct editing events, the lower the likelihood of an...transduction control, a different gRNA would have distinct off-target sites, eliminating its effectiveness...

...chemical input to an RSM drives expression of a distinct large serine recombinase that recognizes its own...demonstrates an example of a register that enters distinct states when treated with different combinations...designs, we have observed that certain GRSMs may still not perform as expected, perhaps due to idiosyncrasies...the same gene regulation program. We recommend testing multiple register designs for the same gene regulation...described in the literature (5, 6) and may be substituted for one another in an RSM. The predictability...

...was fascinated by CRISPR and rushed to test it. I still remember the day, March 16, 2014, that I got my ... in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...transmission of the transgene, this technique is still in need of improvement. Also when working with CRISPR... discovered in an obscure bacterium and that we still have much to learn from biology in all its forms...editing.     Haoyi Wang is a professor at the Institute of Zoology, Chinese Academy of Sciences, and he...

... transcription factors, or when the gene is constitutively-active or a dominant-negative mutant. Reducing... TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely seamless...researcher may subsequently kill off any cells that still contain the donor plasmid after excision by PBx,... technology using a site-specific nuclease to stimulate homologous recombination and Footprint-Free™ removal...and I. M. Verma, “Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from...

...Fellow in the Church and Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is...CRISPR during his postdoc 12:01 - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why .... 13:22 - 22:07 : Alex's projects at the Wyss Institute - New CRISPR tools and the importance of moving...26 - 33:08 : Plans for future work at the Wyss Institute. 33:09 - end : How Alex hopes to help Addgene ...

...far? Do you have any suggestions for this year’s teams that may be procrastinating choosing their costume...these. Addgenies have been organizing Halloween festivities for years – searching our photo archives I found... ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, ...

...service and is ready to use out of the box. We estimate that providing cloning grade DNA could save scientists...Plasmids offered as cloning grade DNA As we begin testing out this service, we’re starting with 210 plasmids...molecular biology experiments such as cloning, digestions, PCR etc. and/or directly long term storage. ...service in the future using the Cloning Grade DNA Suggestion Form.  Additional resources on the Addgene blog...

...our in-stock service: ddPCR, purity testing, and sterility testing, plus viral genomic sequencing to confirm...webpage to get started with your browsing and requesting. If you’re curious about the service, read on...on Request AAVs are a big deal, so if you have questions, we have plenty of information available for you...

... service, AAV Packaged on Request  has seen a fantastic response from the research community. Customers...minBG-jRGECO1a-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14360:...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool AiP14361: ...minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute for Brain Science New viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...

...science policy career and this seemingly simple question is a moving target. There are a multitude of options...policy? I’ve intentionally started with the ‘who’ question because the science policy community is the best...start. What is science policy? THE IMPOSSIBLE QUESTION. Science policy is the work you do to creatively... I would argue that this is the most important question that you can explore. The answer will help you...interviews or essays. Note: the answer to this question may change over time and that’s perfectly normal...

...hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ... ́-phosphate to the ligation). The structural/mechanistic reason for this differential is not known for...Despite this higher fidelity, Taq DNA Ligase can still detectably ligate many T:G, T:T, and A:C mismatches...In this method, substrate pools were prepared consisting of one target (template) strand and four upstream...Reaction Temperature Calculator can be used to estimate an appropriate incubation temperature when using...