We narrowed to 370 results for: SUP

1. Hot Plasmids - August 2020

   Type

   Blog Post

   Published

   Aug. 18, 2020, 1:15 p.m.

   ...of bacteria where it can be secreted into the supernatant. Four plasmids containing different nanobodies...

2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ...noise, of dsDNA breaks caused by CRISPR. See supplemental figure 6 for more details. Safe-targeting guides...

3. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   Published

   Aug. 14, 2018, 12:42 p.m.

   ... imaging technologies such as array tomography, super-resolution STORM imaging, and electron microscopy...

4. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   Published

   April 19, 2018, 1:25 p.m.

   ...out as a student, people tend to respond in a supportive way and are willing to help because you are not...

5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   Published

   July 23, 2019, 12:46 p.m.

   ... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ...

6. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...mediated by the synapsin 1 (SYN) promoter, which was superior in its ability to induce CRISPRa in primary rat...

7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

   Type

   Blog Post

   Published

   May 2, 2019, 12:41 p.m.

   ...number of systems that can be studied by providing support "for development of tools, approaches, and infrastructure...

8. Lab to Office Culture Shock

   Type

   Blog Post

   Published

   Feb. 5, 2019, 1:04 p.m.

   ...anymore, but you will very likely be on a team that supports you when you need it. The opportunity to be ...

9. New CRISPR Tools: Cas7-11 and PASTE

   Type

   Blog Post

   Published

   Feb. 17, 2022, 2 p.m.

   ...RNA editing - and further experiments strongly supported their initial findings.  The team originally suspected...

10. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...or a magnet (magnetic beads), providing a solid support while unbound proteins are washed away.  While ...

11. Viral Vectors 101: Pseudotyping

    Type

    Blog Post

    Published

    April 20, 2021, 2:30 p.m.

    ...Wickersham et al., 2007). This system requires first supplying rabies glycoprotein along with the TVA receptor...

12. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides the missing...

13. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ... resources As Addgene built out our scientific support services, we realized that another availability...

14. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...associated with viral vector-based therapies. REVeRT supports advanced gene expression regulation, enhancing...

15. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

16. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...Approximately two weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

17. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...pemetrexed and cisplatin –because it cuts off the blood supply to fast-growing cancer tumors. In fact, the use...

18. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    Published

    Nov. 3, 2016, 2:30 p.m.

    ...years. Which leads to the question of how we can support researchers as they try to keep up with the growing...

19. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...Labor Thomas Perez, Fair Pay for Postdocs: Why We Support New Federal Overtime Rules, made clear that institutional...

20. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...multiple harvest strategy is that each day you are supplying your producer cells with fresh medium. Daily medium...