We narrowed to 382 results for: SUP

...example), as this can also be perturbed by supraphysiological expression levels. BiFC quantification This...

...LwaCas13a, which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In ...

...successful scientists have outgoing personalities to supplement their intellectual prowess and scientific skills...

...requests. Afterwards, from 2/24/20–3/15/20 (the supposed end of lockdown), request activity appears to ...

...notice typically should include the paper, any supplemental data, software repositories, or other information...

...Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins...

...sequences), so enzyme choice is critical. May not support in-frame fusions of coding sequences Sladitschek...

...reaction (primers, dNTPs, polymerase) using the supernatant of lysed bacteria as template; and 3) run your...

...oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification of specific DNA sequences. Thus...

...fraction of one of the four bases (A, C, T, or G) is supplied as a dideoxyribonucleoside triphosphate (ddNTP...

...vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides the missing...

... resources As Addgene built out our scientific support services, we realized that another availability...

...associated with viral vector-based therapies. REVeRT supports advanced gene expression regulation, enhancing...

...Approximately two weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

...noise, of dsDNA breaks caused by CRISPR. See supplemental figure 6 for more details. Safe-targeting guides...

... imaging technologies such as array tomography, super-resolution STORM imaging, and electron microscopy...

... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ...

...mediated by the synapsin 1 (SYN) promoter, which was superior in its ability to induce CRISPRa in primary rat...

...of bacteria where it can be secreted into the supernatant. Four plasmids containing different nanobodies...