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We narrowed to 458 results for: Spr;

Showing: 321 - 340 of 458 results

  1. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...

  2. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...culture in the present day Cell culture is a widespread tool used in the fields of oncology, virology...Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about...

  4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...Mutations in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate Mutant Populations...Addgene.org Find Plasmids from the Whitehead Lab Use CRISPR Pooled Libraries to Generate Mutants ...

  5. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... Mice can be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila...of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies are also...

  6. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic...

  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations..., B.E., Gradinaru V. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nature...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology...

  9. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage for Antimicrobial Purposes...Systems to Probe Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org...

  10. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...RNAi to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate...the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large...

  11. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...each serotype also exhibits distinct patterns of spread from the injection site it is advisable to try ...off-target expression making it easier to determine the spread pattern around the injection site. Test the promoter...

  12. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...without telling you anything about outliers or data spread within those data sets. Measures of dispersion...allowance in a statistical test. Distribution - spread of data; typically, normal (bell curve) or skewed...

  13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...by guest blogger Derek Simon. Optogenetics is spreading through the neuroscience community like wildfire...identification of opsin expression, extent of viral spread, and with co-localization immunofluorescence, cell-type...

  14. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...implanting them into female mice. Generating chimeric offspring shows the ability of the iPSC to contribute to... theses chimaeras to produce all-iPSC derived offspring demonstrates the ability of the iPSCs to form ...

  15. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...analyzing qPCR data, most with plug-and-play Excel spreadsheets available for easy analysis. These useful tools... vol. 1160, DOI 10.1007/978-1-4939-0733-5_3, © Springer Science+Business Media New York 2014Bustin S; ...

  16. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...eBook webpage.  More resources on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition...

  18. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your ...

  19. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...seminal” studies for various categories, including CRISPR gene-editing, the foundations of synthetic biology...
Showing: 321 - 340 of 458 results