We narrowed to 746 results for: TRI

...,850 SARS-CoV-2 plasmids to help academic and industrial labs accelerate research and to help find a faster...citation to the plasmid page on our website. Contributing your results helps other researchers using these...high throughput platforms, Addgene and Ginkgo are striking a balance by modifying certain processes in both...

... adenine base editor Lower-stringency PANCE, followed by higher stringency PACE, resulted in the evolution...which results in less overall dilution and less stringent phage selection. Figure 2: Phage-assisted...

...with biorender.com. ITR structure  WT ITRs are symmetrical 145-nucleotide sequences that flank the ends ...identification of non-dominant ITR sequences.  Restriction digests with the enzymes SmaI and SrfI can also...deletions and point mutations will go undetected by restriction digest methods.    Resources and references...

...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...sample. Practical brightness A protein with a high intrinsic brightness that does not fold well or cannot be...explanation of the assay is depicted in figure 1. The strict correlation between the fluorescent protein of ...

... This post was contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad...works. But, those darn cancers have revealed many tricks up their sleeves and most patients still don’t ...or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary conservation...

...These viruses have been distributed in over 200 packages to more than 20 countries. With this initial success...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...

... a biotech startup company? Would you like to contribute advice on how to effectively raise money and/... Capital in Biotech  - Posted on Forbes.com, contributed by Bruce Booth How to Find a Job in Biotech and...the Biotech Business - Posted on Nature.com, contributed by Julia Phillips and Fiona Beckman Acknowledgments...

...real-time inside a mouse’s brain.  But the brain is a tricky organ to access, which often forces scientists ...electrophysiology, a technique that’s used to measure electric current of ion channels in living cells. Multiple...-stimulate” their VTA dopaminergic neurons by triggering a burst of light that could activate ChRger2 ...

...plasmid, excluding any restriction enzymes or vector purchases (Table 1). Restriction enzymes and vector ...work done previously, borrow small aliquots of restriction enzymes for one-off projects, or even save yourself...

...This post was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "...interleukin-6 (Witkiewicz et al., 2009) which contribute to the development and spread of cancer cells... scientists began focusing on the extracellular matrix and fibroblasts. With each cell mechanism discovered...

...off-target effects attributable to CRISPR editing. Conclusions & implications One striking feature of this...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

... held from 22-24 October. This blog has been contributed by guest blogger and Future of Research Symposium...the academic labour market. Her many notable contributions in this area include publishing the book ‘How...How Economics Shapes Science’ and contributing to the NIH’s Biomedical Research Workforce Working Group...

...This post was contributed by Neil J. McKenna and Bert W. O’Malley. Survival of all organisms depends ...organs, which then return information such as nutritional status. Within the animal kingdom, this information...300 different coactivator proteins individually contribute to cell regulation in a coherent manner. Because...

...recommend checking your cloning via a miniprep and restriction digest, and then plasmid sequencing if you can...Cloning specifics Fragmid uses Bbsl, a Type II restriction enzyme that cleaves outside of its recognition..., 93% had the correct banding pattern in the restriction enzyme digest. They sent 82 of the correctly ...

...Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular cell types...types, pairs of synthetic promoter can be used to restrict expression to a single cell type based on the ...cell-type specific promoters could be an easier way to restrict expression of a gene interest in an animal model...

...Scholar library. You can add hashtags to library entries, which can help you organize and search for papers...number of times a paper is shared online, or its altmetric score, is also displayed in your feed. By clicking...us know if you if you have any favorite tips and tricks for navigating the always growing sea of scientific...

...generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which they named...with a FLAG tag (!), but did you know that we distribute ready-to-use recombinant antibodies as well? ...anti-FLAG antibody, both of which are also available in trial sizes. Addgene recommends these recombinant antibodies...

...This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics...Education Project (pgEd), where I currently work, strives to broaden the conversations about genetics and...implications of genetics. In these conversations, pgEd strives to be inclusive of all voices, regardless of socioeconomic...

...the G receptor. In neurons, the G receptor is restricted to presynaptic nerve terminals (Lafon 2005). ...applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a powerful development...used to reveal brain circuitry, and continue to contribute answers to the complex questions of how the brain...

...Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at the Allen Institute...regions of high homology to the gene with the cut, we trick the cell into using the introduced donor plasmid...Allen Institute for Cell Science. This post was contributed by the gene editing team at the Allen Institute...