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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 743 results for: TRI

Showing: 321 - 340 of 743 results

  1. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ... This post was contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad...works. But, those darn cancers have revealed many tricks up their sleeves and most patients still don’t ...or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary conservation...

  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...These viruses have been distributed in over 200 packages to more than 20 countries. With this initial success...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...

  3. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...developed in terms of the collection, storage, and distribution of the plasmids and their ability to identify...plasmids so scientists aren’t bogged down with distributing them and spending more time on research. What...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...

  4. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ... a biotech startup company? Would you like to contribute advice on how to effectively raise money and/... Capital in Biotech  - Posted on Forbes.com, contributed by Bruce Booth How to Find a Job in Biotech and...the Biotech Business - Posted on Nature.com, contributed by Julia Phillips and Fiona Beckman Acknowledgments...

  5. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...plasmid, excluding any restriction enzymes or vector purchases (Table 1). Restriction enzymes and vector ...work done previously, borrow small aliquots of restriction enzymes for one-off projects, or even save yourself...

  6. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...real-time inside a mouse’s brain.  But the brain is a tricky organ to access, which often forces scientists ...electrophysiology, a technique that’s used to measure electric current of ion channels in living cells. Multiple...-stimulate” their VTA dopaminergic neurons by triggering a burst of light that could activate ChRger2 ...

  7. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...off-target effects attributable to CRISPR editing. Conclusions & implications One striking feature of this...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

  8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...This post was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "...interleukin-6 (Witkiewicz et al., 2009) which contribute to the development and spread of cancer cells... scientists began focusing on the extracellular matrix and fibroblasts. With each cell mechanism discovered...

  9. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... held from 22-24 October. This blog has been contributed by guest blogger and Future of Research Symposium...the academic labour market. Her many notable contributions in this area include publishing the book ‘How...How Economics Shapes Science’ and contributing to the NIH’s Biomedical Research Workforce Working Group...

  10. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...This post was contributed by Neil J. McKenna and Bert W. O’Malley. Survival of all organisms depends ...organs, which then return information such as nutritional status. Within the animal kingdom, this information...300 different coactivator proteins individually contribute to cell regulation in a coherent manner. Because...

  11. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...recommend checking your cloning via a miniprep and restriction digest, and then plasmid sequencing if you can...Cloning specifics Fragmid uses Bbsl, a Type II restriction enzyme that cleaves outside of its recognition..., 93% had the correct banding pattern in the restriction enzyme digest. They sent 82 of the correctly ...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which they named...with a FLAG tag (!), but did you know that we distribute ready-to-use recombinant antibodies as well? ...anti-FLAG antibody, both of which are also available in trial sizes. Addgene recommends these recombinant antibodies...

  13. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...the G receptor. In neurons, the G receptor is restricted to presynaptic nerve terminals (Lafon 2005). ...applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a powerful development...used to reveal brain circuitry, and continue to contribute answers to the complex questions of how the brain...

  14. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular cell types...types, pairs of synthetic promoter can be used to restrict expression to a single cell type based on the ...cell-type specific promoters could be an easier way to restrict expression of a gene interest in an animal model...

  15. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...Scholar library. You can add hashtags to library entries, which can help you organize and search for papers...number of times a paper is shared online, or its altmetric score, is also displayed in your feed. By clicking...us know if you if you have any favorite tips and tricks for navigating the always growing sea of scientific...

  16. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at the Allen Institute...regions of high homology to the gene with the cut, we trick the cell into using the introduced donor plasmid...Allen Institute for Cell Science. This post was contributed by the gene editing team at the Allen Institute...

  17. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics...Education Project (pgEd), where I currently work, strives to broaden the conversations about genetics and...implications of genetics. In these conversations, pgEd strives to be inclusive of all voices, regardless of socioeconomic...

  18. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ...findings will be worth knowing? This can sometimes be tricky, as most of our research builds on ideas and findings... realization that, if we want people to keep contributing to science, it would be helpful for them to ...

  19. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided...differing from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing ...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

  20. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering...with very little money. So when did inspiration strike us so hard that we upended our lives and took this...things we could do for work, and they weren’t restricted to science jobs. Keira’s an optimist, I’m a realist...
Showing: 321 - 340 of 743 results