We narrowed to 625 results for: UTY;
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TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be a funky...proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m.... T helper type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect...commonly called bad cholesterol). However, size distribution and concentration of ApoB-LPs has been shown... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....we can also learn a lot from Nature’s simpler solutions to transmitting forces over long distances. For... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....excited about the prospect of working toward a solution. At the end of the meeting, make sure to thank...Addgene Blog Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m.... the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs were then verified...plasmids have been deposited with Addgene for distribution to the scientific community with the plasmid... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....genetic manipulations. You can produce some very beautiful immunofluorescent images like in this paper (2...experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....each genotype with vulnerabilities) to design a therapeutic strategy that works. But, those darn cancers ... using attributes of cancer variants such as evolutionary conservation, or predicted deleteriousness of... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m.... break site (Figure 2A). The single molecule resolution of digital PCR allows classification of individual...total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA from a few thousand... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....more effectively used in a technology or as a therapeutic. A (very) brief history GCE came onto the synthetic...to include reagents accessible to your lab. The solution GCE provides almost endless possibilities for ... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....gamma-retrovirus in the lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival...naturally occurring and more synthetic capsids are routinely being developed) to choose from, which can target... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....serotype also has promising therapeutic applications; it can enhance therapeutic delivery across broader ... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m.... "do it yourself" can result in burnout and underutilized team potential. I often thought that performing...PhDs in their careers with actionable steps and execution plans. Resources Resources for aspiring managers... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog PostPublishedDec. 12, 2019, 2:22 p.m....protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....this same antibody to any protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small...thing to do to put these out there. It’s a simple solution. It would be silly not to share it as soon as ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve...multiplexing afforded by PTGs will improve the utility of CRISPR/Cas9 in new avenues of research. The ... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog PostPublishedJuly 10, 2014, 7:17 p.m....bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding proteins that...proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m...., broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....alike. We transformed this process by aligning institutions with standardized agreements and electronic ...Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some of our ...