We narrowed to 484 results for: Vit
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TypeBlog PostPublishedJune 5, 2017, 3:19 p.m.... our culture and outlook: Addgene increases productivity by providing world-wide access to curated, high-quality...
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SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....traditional FP fusions can also affect protein activity, half-life, or localization, these concerns are... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....presented my research to Addgenies and friends. These invited guests came to see the research from all the high... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....sensitive proteins (opsins) to manipulate cell activity, enables researchers to silence or incite neuronal... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....authors show that HcKCR1 and HcKCR2 have high selectivity for K+ over Na+ and rapid photocurrent kinetics... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....Isaacman-Beck et al., 2019. Sparse Predictive Activity through Recombinase Competition (SPARC), a genetic... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....Region Drives transcription of the target gene. Vital component for expression vectors: determines which... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m.... TRUPATH as a screening technique. Lastly, the activity of both antagonists and inverse-agonists could... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....element (RBE) in the ITRs, uses its endonuclease activity to generates a ssDNA substrate which is then nicked... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m....with a little mutagenic assistance). Quicker CAST activity allowed certain mutated phages to outcompete their... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m....bacteria to grow on sucrose. Plasmid cloning: Creativity with naturally occurring genes At this point... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m....“blue flame” status. We’re proud to honor the creativity, passion, and dedication to sharing epitomized... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog PostPublishedNov. 1, 2016, 2:30 p.m....understood, it is believed that the exonuclease activity of the polymerase generates sticky ends in the... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts resulted in... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m....discussion, and/or brainstorming sessions to maximize productivity and direction of the project? What is your ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....address far and wide, keep your website up to date, invite people to ask questions, be approachable. Engage... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my sentences might sound... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....bright, monomeric green fluorescent protein. In vitro, mWasabi is 1.6-fold brighter than EGFP and is photostable... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....also observed reliable optically evoked neuron activity for at least several months after implantation...