We narrowed to 487 results for: Vit

1. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

   Type

   Blog Post

   Published

   Aug. 22, 2019, 12:43 p.m.

   ...bacteria to grow on sucrose. Plasmid cloning: Creativity with naturally occurring genes  At this point...

2. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

   Type

   Blog Post

   Published

   July 16, 2019, 12:59 p.m.

   ...sensitive proteins (opsins) to manipulate cell activity, enables researchers to silence or incite neuronal...

3. An Addgene Summer Internship

   Type

   Blog Post

   Published

   Aug. 8, 2024, 7:22 p.m.

   ...presented my research to Addgenies and friends. These invited guests came to see the research from all the high...

4. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

   Type

   Blog Post

   Published

   June 19, 2018, 1:38 p.m.

   ...reporter genes for in vivo imaging Following in vitro characterization, Bourdeau et al performed a proof-of-concept...

5. Understanding GPCR Signaling Using TRUPATH

   Type

   Blog Post

   Published

   Dec. 14, 2020, 2:15 p.m.

   ... TRUPATH as a screening technique. Lastly, the activity of both antagonists and inverse-agonists could...

6. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   Published

   Dec. 3, 2020, 2:15 p.m.

   ...element (RBE) in the ITRs, uses its endonuclease activity to generates a ssDNA substrate which is then nicked...

7. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

   Type

   Blog Post

   Published

   July 18, 2017, 12:41 p.m.

   ... produce compounds with various antimicrobial activities (1). She has developed a fast endophyte screening...

8. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

   Type

   Blog Post

   Published

   June 5, 2017, 3:19 p.m.

   ... our culture and outlook: Addgene increases productivity by providing world-wide access to curated, high-quality...

9. Hot Plasmids - October 2022

   Type

   Blog Post

   Published

   Oct. 6, 2022, 1:15 p.m.

   ...authors show that HcKCR1 and HcKCR2 have high selectivity for K+ over Na+ and rapid photocurrent kinetics...

10. SunTag and Fluorescent Imaging

    Type

    Blog Post

    Published

    March 28, 2017, 2:30 p.m.

    ...traditional FP fusions can also affect protein activity, half-life, or localization, these concerns are...

11. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type

    Blog Post

    Published

    Nov. 14, 2016, 3:30 p.m.

    ...“blue flame” status. We’re proud to honor the creativity, passion, and dedication to sharing epitomized...

12. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type

    Blog Post

    Published

    Dec. 12, 2017, 3:13 p.m.

    ...RNA target. Bonus: It displays similar on-target activity to siRNA, but with far fewer off-target effects...

13. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type

    Blog Post

    Published

    May 21, 2020, 1:15 p.m.

    ...Isaacman-Beck et al., 2019. Sparse Predictive Activity through Recombinase Competition (SPARC), a genetic...

14. Plasmids 101: What is a plasmid?

    Type

    Blog Post

    Published

    April 2, 2020, 6:17 p.m.

    ...Region Drives transcription of the target gene. Vital component for expression vectors: determines which...

15. FastCloning: A Newer, Simpler Cloning Technique

    Type

    Blog Post

    Published

    Nov. 1, 2016, 2:30 p.m.

    ...understood, it is believed that the exonuclease activity of the polymerase generates sticky ends in the...

16. Sleeping Beauty Awakens for Genome Engineering

    Type

    Blog Post

    Published

    June 30, 2015, 2 p.m.

    ...lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts resulted in...

17. Advice for Choosing a Research Project

    Type

    Blog Post

    Published

    April 17, 2014, 2:45 p.m.

    ...discussion, and/or brainstorming sessions to maximize productivity and direction of the project? What is your ...

18. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    Published

    Jan. 30, 2014, 3:29 p.m.

    ...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media...

19. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type

    Blog Post

    Published

    Nov. 12, 2019, 2 p.m.

    ...time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved...

20. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type

    Blog Post

    Published

    Aug. 8, 2019, 1:19 p.m.

    ...non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my sentences might sound...