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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 707 results for: ang

Showing: 321 - 340 of 707 results

  1. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and grown ...much learning, the focus of the application was changed, but Sample6 still leverages the core engineering...better than current technology and get customers to change behavior. When you are ready to commit to the company...

  2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...can be hard to visualize or perturb these slower-changing bioelectric states in living cells. Since bioelectrical...PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction protein-mediated cues control... Angela Abitua...

  3. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ... in D.C. for a few years, Susan was ready for a change of scenery. She moved to Seattle and continued ... there there’s always plenty of writing: plain-language stories about Fred Hutch research, pieces for ... scientist or someone who’s affected by climate change or affected by a superbug. I think we solve these...

  4. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available for antibody conjugation...and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based... choice for ELISAs.  Biotin Biotin is used in a range of antibody-based assays. Biotin itself is not visible...

  5. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...plasmids modified from pSC101 with copy numbers ranging from 3-100 per cell, a stabilized promoter buffered... On the other hand, slow growth can also cause changes in plasmid copy number. During the shift from exponential... Jennifer Tsang...

  6. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...nutrient and ionic balance of the cells,” says Dr Sangeetha Iyer, ACD’s Scientific Advisor. It’s possible ...Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when I was a newly ... Jennifer Tsang...

  7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...are proteins that detect and output the voltage changes in neural activity when paired with appropriate...optogenetic actuators.  To overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted...

  8. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...sure the feature you want to verify is within this range. Read some of our other blog posts for more tips... simplify the process. First, nearly all of our Sanger sequencing is done from glycerol stocks (overnight..., and not all providers are equipped to perform Sanger sequencing from glycerol stocks, so make sure to...

  9. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ... loci, and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved the on-target...CRISPR/Cas9 genome editing can result in unwanted changes at non-target sites. Means to decrease these so-called... Recognizing these issues, researchers from the Zhang lab at the Broad Institute, the Joung lab at Harvard...

  10. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...are for the coaching relationship. Do you want to change jobs? Grow your professional presence? Learn a ...and make direct suggestions for improvement and change. Work with the coach to define how you will know...work with you remotely (by phone, Skype or Google Hangout) but in-person coaching is nice if you can get ...

  11. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... govern expression of genes involved in a broad range of reproductive, developmental, metabolic, and immune... expression and reporter plasmids and the free exchange of these vectors between laboratories. The development...Reading: Chawla, A., Repa, J. J., Evans, R. M.and Mangelsdorf, D. J. (2001). Nuclear receptors and lipid physiology...

  12. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...common and will stand out; however, be careful not to hang your poster too low. If it is pinned to low, some...inserted into larger circles or ovals, that are arranged in some logical pattern across the poster. This...into circular shapes rather than a square or rectangular boxes. Background and color One of the best ...

  13. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...the glycan biosynthetic pathway and have a wide range of clinical manifestations. PMM2-CDG is the most...cause of rare diseases. Spontaneous mutations that change protein sequence affect arginine (Arg) amino acids...missense mutant alleles that cause human disease change Arg to another amino acid. Clearly, the development...

  15. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...Virus-specific concerns   In nature, some viruses are more dangerous than others, and the same holds true for types...For many researchers, viruses might be the most dangerous things they work with, and that can understandably...let you work with these valuable tools without endangering yourself or others. Happy transducing! Resources...

  16. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...information briefs for policymakers. The rapidly changing landscape of genetics and our consistent stream...society, and an ability to talk about science in a language that can be understood by those who are not experts...communicate in very technical and jargon-filled language in academic writing and presentations. But for...

  17. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...experiment. References 1. Kutner, Robert H., Xian-Yang Zhang, and Jakob Reiser. "Production, concentration... 16836756. PubMed Central PMCID: PMC1534021. 3. Zhang, Bing, et al. "The significance of controlled conditions...

  18. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...the only right way, and Indecisives, who keep changing their minds and have trouble making decisions....talking about your emotions, such as frustration or anger, because your discussion will get side-tracked, ...instead of email. As you cannot see a person’s body language or sense their tone of voice in an email, there...

  19. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...overwhelming when you are presented with a sea of strangers. Try things like sitting at a table for lunch ... for myself like meet ten new people each day. Exchange business cards. When you’re done networking for... Jennifer Tsang...

  20. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...thus a heat resistant enzyme that can withstand changes in temperature. Taq was first identified in the...Annealing- The temperature is then dropped to a range in which the forward and reverse primers are stable...Quantifying low abundance targets Monitoring subtle changes in target DNA levels Multiplex PCR Amplify...
Showing: 321 - 340 of 707 results