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  2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... undergoing two rounds of gene-editing enables scarless introduction of an intended mutation while taking...4. Kwart, Dylan, et al. "Precise and efficient scarless genome editing in stem cells using CORRECT." Nature...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...First, CRISPR would need to be delivered to both cardiac and skeletal muscle cells, where precision editing... each observed the same positive phenotypes in cardiac and skeletal muscle. Even when the genome editing...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...intrinsic selectivity of the ligase active site and careful balance of reaction conditions to reduce the incidence...Probes), use of a high fidelity ligase, along with careful probe design and reaction condition optimization...

  6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...cassette minimizes the sequence added, leaving a 34 bp “scar”, but currently requires additional experimental...efficiencies ranging from 11-100% of F1 animals carrying precise knock-ins, and homozygous knock-in animals...

  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x and tween...while a droplet containing a negative cell is discarded. FACS can be performed aseptically allowing users...

  8. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...limitations or that other technologies need to be discarded but clearly we are in an era of neuroscience research... as the vector core at the University of North Carolina) that offer his constructs. Importantly, control...

  9. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...parts of the Cas9 enzyme.   Figure 1: A cartoon depiction of Cas9’s two major lobes, REC and NUC...process from start to finish:   Figure 9: Cartoon summary of Cas9 activity. The apo enzyme is shown...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... PubMed Central PMCID: PMC5529245. Choi, V.W., McCarty, D.M., and Samulski, R.J. (2005). AAV Hybrid Serotypes... and Holzbaur, E.L.F. (2014). Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation. ...

  12. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ... again this year! All winners receive an Addgene care package with Addgene mugs and some delicious hot...

  13. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ... de nuestra colección de libros electrónicos. Descargar CRISPR 101: Una herramienta de edición Addgene...

  14. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Summer Internship Matteo Tardelli, who added to our career advice collection. Embracing Serendipity Nicolas...

  15. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ... know? Fill out our referral form and we’ll take care of the rest. Know of a useful plasmid that’s not...

  16. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new plasmid tool your...

  17. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, plasmids. Today...
Showing: 321 - 340 of 523 results