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...This post was originally written by Maria Soriano-Carot in September 2016 and was updated by Rachel Leeson... career...

... the cell type of interest (Atasoy et al. 2008; Cardin et al. 2009). Ligand regulated recombinase - keeping...biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking ...

...invaluable reagents with the scientific community. Note: Cary Valley and Mary Gearing contributed to the update...PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID-19 using SHERLOCK diagnostics....

...antibiotics is certainly a topic of great concern in healthcare today, but it doesn’t have to be some looming...resistance from the CDC. Antibiotic resistance can be scary to think about, but we can all do our part to learn...

...promoter on the plasmid. Many commercial strains carry the DE3 lysogen, as indicated by the name of the...reasonable time-frame. For these cases, some strains carry an additional measure of control such as the pLys...

...coupled with your technical skills, can open new career opportunities for you, if you can identify them...eye out for the phrase “I assumed they were taking care of that”, because that can indicate confusion around...

...available resources and give yourself time to carefully review your options. If possible, try to select...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

...Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer with 9V battery Approach...fluorescently labelled RNA, the authors employ a P51 cardboard fluorescence visualizer that is powered by a 9V...

...Learn about Careers in Science Communication Resources at Addgene.org Check out Careers at Addgene ...

... undergoing two rounds of gene-editing enables scarless introduction of an intended mutation while taking...4. Kwart, Dylan, et al. "Precise and efficient scarless genome editing in stem cells using CORRECT." Nature...

...issues Reproductive health Immigration Pollution Healthcare Science education To make scientists relatable...Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is not 'primary contributor' to global ...

...cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry. Keep the linker sequence short...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...

...First, CRISPR would need to be delivered to both cardiac and skeletal muscle cells, where precision editing... each observed the same positive phenotypes in cardiac and skeletal muscle. Even when the genome editing...

...cassette minimizes the sequence added, leaving a 34 bp “scar”, but currently requires additional experimental...efficiencies ranging from 11-100% of F1 animals carrying precise knock-ins, and homozygous knock-in animals...

...intrinsic selectivity of the ligase active site and careful balance of reaction conditions to reduce the incidence...Probes), use of a high fidelity ligase, along with careful probe design and reaction condition optimization...

...educational science led her to pursue a teaching career, and she now is a very happy scientific communication...Samulski. "Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological...

...detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x and tween...while a droplet containing a negative cell is discarded. FACS can be performed aseptically allowing users...

...limitations or that other technologies need to be discarded but clearly we are in an era of neuroscience research... as the vector core at the University of North Carolina) that offer his constructs. Importantly, control...

...parts of the Cas9 enzyme.   Figure 1: A cartoon depiction of Cas9’s two major lobes, REC and NUC...process from start to finish:   Figure 9: Cartoon summary of Cas9 activity. The apo enzyme is shown...

... PubMed Central PMCID: PMC5529245. Choi, V.W., McCarty, D.M., and Samulski, R.J. (2005). AAV Hybrid Serotypes... and Holzbaur, E.L.F. (2014). Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation. ...