Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 811 results for: cha

Showing: 321 - 340 of 811 results

  1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...site. Cutting the linker by a caspase results in a change in fluorescence emission. FRET and fluorescence..., and thus quantify, due to small fluorescence changes of the donor and acceptor after proteolytic cleavage...fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence, cell... cell heterogeneity, and/or cell position changes (To et al., 2016, Zhang et al., 2019). Thus to effectively...

  2. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...information they’ve absorbed if they don’t get a chance apply it in an interactive setting. The problem...could be played when and, depending upon who your challenger was, you might have to drastically alter your...please freely share your projects with us! This exchange of ideas will help improve life science and biomedical...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...complicated entities with a large range of characteristics (color, brightness, photostability, maturation...attach important references that serve to further characterize a protein of interest (and we very much appreciate...users to see when a given page has unmoderated changes and to view previous versions.  Currently, all ... plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein Collection ...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...Skip to Chapter 3 for tips on how to develop your role as a lab leader, and then on to Chapter 4 for advice...the influence from his two PhD advisers who were “chalk-and-cheese”. While one was an established Professor....” Take home message Starting a lab can be a challenging process. Each PI emphasizes the importance of...

  5. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...our mission, it’s also because Addgenies love any chance to nerd out over their favorite science topics....Addgenie expertise we can. We even have a Slack channel just for asking people if they can write or review...posts get out of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, ..., for example, has gone through rapid change in the past few years. Thus, we are constantly updating our...

  6. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...available in your machine before you make your purchase. Picking a single fluorophore for an experiment...overlap, or the signal "spilling over" into another channel's detection range. Though you can compensate for... University Flow Core. Fluorochromes Brightness Chart. Boston University Flow Core. Retrieved January ...flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf Syracuse Flow Core. Syracuse flow core spectral...

  7. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...overall positive charge on DNA molecules so that it can more easily cross the negatively charged cell membrane...role in bacterial evolution and is the primary mechanism by which bacteria have gained antibiotic resistance...electroporation, the application of an electrical charge to cells, to increase cell membrane permeability...

  8. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ... of bioluminescence is the firefly. Along with charming nighttime displays, these insects have contributed...bright. Other luciferases have been modified to change the wavelength of light they emit or to optimize...interaction occurs, the luciferase emits its characteristic light. However, if a protein interaction brings...been used in biosensors where BRET reports on the change in conformation of a single protein. Considerations...

  9. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ..., but they do include HEPN nuclease domains characteristic of the Cas13 superfamily. Yan et al. proceeded...Ruminococcus sp. (RspCas13d), while Konermann et al. characterized orthologs from “Anaerobic digester metagenome...Cas13a and Cas13b effectors. Like previously characterized Cas13 enzymes, CasRx also displays very high...Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org: Find RNA Knockdown...

  10. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...Immunologists are coming together to tackle the challenge of curing cancer in cross-disciplinary projects...projects and collaborations. Summarized by Professor Michael Karin from UCSD: “The problem with cancer research...poster sessions During the poster session I got a chance to talk with Dr. Friedmann-Morvinski about tools...27411589. 2. Todoric, Jelena, Laura Antonucci, and Michael Karin. "Targeting inflammation in cancer prevention...

  11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...and γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (... vice versa. Envelope plasmids may be used interchangeably. Are γ-retroviruses replication incompetent...References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses ...cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: 10.1002/0471142735.im1017cs31...

  12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ... Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks Article contributed...equipment. What’s more, detection is based on color changes to the assay paper, so no special equipment or ...novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3 domain podcast segment...biochemical processes. One of the largest and best characterized of these is the Src homology 3 (SH3) domain ...

  13. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...Roth’s lab at the University of North Carolina, Chapel Hill. These can be used to investigate the effects...transgene expression across different cell types (Aschauer et al., 2013). Expression of hM4D(Gi)-mCherry ...hM4D(Gi)-mCherry in fixed tissue. References 1. Aschauer DF, Kreuz S, Rumpel S. Analysis of transduction...

  14. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...that further helps you keep track of your exact changes. Alternatively, you can simply make a note in your...

  15. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and grown ...much learning, the focus of the application was changed, but Sample6 still leverages the core engineering...his co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...better than current technology and get customers to change behavior. When you are ready to commit to the company...

  16. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...kits or ordered as synthesis modifications when purchasing your gRNA. Pro tip: modifications are typically...expression systems are constantly “on”. There is no mechanism to turn cutting off or on at a specific time. ...and you can opt to do-it-yourself with a kit or purchase it commercially synthesized with a label. Not ..., E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based...

  17. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...scientists communicate with each other: from formal mechanisms like publishing a research article, presenting...settings like lab meetings, social media, coffee chats with potential collaborators, blogging and more....read freely by anyone and are typically free of charge for authors to post. They can be assessed and commented...authors over time, providing a public record of changes, critiques or endorsements. In contrast, journal-based...

  18. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ... antibody cross-reacts to, then there is a good chance that the antibody could meet your needs.    Multiplex...will not only have peace of mind as you make your purchase but also someone to turn to for advice if you ...application.      Fig. 1: Antibody Selection Flow Chart    What does validated mean anyway? You now ...Validation!  Download our antibody selection flow chart.  References and Resources Additional Resources...

  19. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...protein homodimerization is characterized by an affinity of 110 µM (Zacharias et al, 2002), meaning that... proteins reported in publications are only characterized under a limited number of conditions, often ...microscopy (University of Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes...

  20. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ... studying protein turnover is radioactive pulse-chase experiments. However, these experiments are problematic...synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of Sciences...PubMed Central PMCID: PMC4414919. 5. Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of...Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability." Science 343.6169...
Showing: 321 - 340 of 811 results