We narrowed to 872 results for: fer

...transition from a clump of cells to a fully differentiated body. In contrast, these dynamic processes ...distinguishing cell populations that have been tagged with different fluorescent proteins or dyes. 3. Two-photon microscopy...cells. For example, by harvesting lymph nodes at different times after antigen challenge, scientists were... see movement and interaction between several different immune cells. These methods allowed immunologists... like this is a mosaic of cells labelled with different fluorescent proteins. Using one iteration of this...understanding of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging...17145958. PubMed Central PMCID: PMC2118178. 6. Schaeffer, Marie, et al. "Dynamic imaging of T cell-parasite...

...created a generalizable tag-based strategy, which we refer to as the degradation tag (dTAG) system, to co-opt...selectivity, broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG...expression of these cereblon neo-substrates varies in different contexts (for example, IZKF1 and IZKF3 are cell-type...technology. Follow him on twitter @behnamnabet.   References Nabet et al. The dTAG system for immediate and... 14(5): 431-441. PubMed PMID: 29581585. This reference contains the full description of the dTAG system... Huang et al. MELK is not necessary for the proliferation of basal-like breast cancer cells. eLife. September...

...a property of the fluorescent protein. This can differ between fluorescent proteins in the same spectral...dependence on excitation power is important since different fluorescence imaging strategies use widely varying..., TIRF or spinning disk confocal use entirely different excitation power regimes. Therefore, it is unpredictable...the best way to compare the photostability of different fluorescent proteins? Measuring photostability...excitation power. By repeating these measurements for different fluorescent proteins and by comparing how the ...measurements reported in the literature are performed in different ways. We describe a number of issues with some...

... These each have different goals, formats, and processes, and they provide different amounts of access...research during his PhD studies.  References and Additional Resources References ASAPbio, Preprint Resource ...like publishing a research article, presenting a conference poster or talk, or submitting patent or grant...Going to present some unpublished results at a conference? Post your working manuscript as a preprint too...

...sources. Mode of communication Different modes of communication take up different amounts of mental bandwidth...definition of cognitive load states: “cognitive load refers to the used amount of working memory resources....with a specific topic; extraneous cognitive load refers to the way information or tasks are presented to... to a learner; and germane cognitive load refers to the work put into creating a permanent store of knowledge...clearly as you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

... hand and use them to easily test different antibodies or different signaling molecules. Many labs find...antibodies to bind to a primary antibody without interfering with the hypervariable region, where an antibody...are readily available conjugated to a number of different signaling molecules. With the direct approach,...possible, or ensure that the primary antibodies are different species and/or isotypes. For highly multiplexed...generated in goat against rabbit immunoglobulins. References and Resources More resources on Addgene’s blog...

...poised for more offerings. The viral vector team is publishing research, hosting conferences, and are expanding...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received...step to maximize the services and features we can offer online for Addgenies and to serve external customers... a lot of successful candidates from internal referrals. Our long reaching reputation helps us hire great...practices and benefits and these are making a difference. We serve scientists around the globe and our...

...scientific manuscript will require numerous other references to scientific literature to substantiate the ...literature related to the topic. Finding reliable references and sorting out what they mean is no small task...Did they perform all of the appropriate controls? Refer back to the methods section for finer points of ...inspiration and accountability. There are a few different ways to automate the process as well. Sign up ...with scribbled notations in the margins. If you prefer, printing and hand-writing notes is still possible... Check out Papers and Mendeley – these programs offer more options for searching and sorting your own ...

...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...years evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely used...distinct complexes may explain how more than 300 different coactivator proteins individually contribute to...patterns of nuclear receptors across multiple different tissues in normal and disease states, their circadian...

... one. As a starting point, I would recommend conferences like Lab Robotic Interest Group (LRIG). There...customizability. It has a large deck that can house many different types of devices. As long as a device fits on ... - one pod can pick up 96 tips for easy sample transfer between 96 well plates. The second pod can pick...out your samples. This can be useful if you’re transferring samples from 96 wells plates onto agar q-trays...follows all the same steps and uses the similar buffers as would be used for a mini prep, except it does...video library Check out our molecular biology reference pages ...

...plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab produce different levels of dCas9 expression...system developed to track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a ...cell surface. When the cells interact, GFP is transferred to the receiver cell, and the receiver cell turns.../genetics/iyab006       The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith Joung...

...some of Cas9’s shortcomings. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both...pattern opens up the possibility of directional gene transfer, analogous to traditional restriction enzyme cloning... cloning. Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells...immunogenicity and range of serotypes allowing preferential infection of certain tissues. However, these...Cpf1 fits into the genome editing landscape. References 1. Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim...

...subtypes of A–G, which come from different species and will have different compositions of Cascade. Although...degradative power in the future! References and Resources References Loeff, L., Brouns, S. J., & Joo, ... archaea. Class 1 is further broken down into different types, with Cas3 systems making up type I, the...effector. Type I has subtypes A through G, with different compositions of the Cascade complex. Created with...

...focus on gene editing techniques. References and resources References Akhtar, W., De Jong, J., Pindyurin...means we could design hundreds to thousands of different pegRNAs for each desired edit, it simply becomes...many more. Once we had our datasets with all the different features and editing efficiencies, we could train...offline via GitHub. The model provides users with different designs of pegRNAs, ranked by their PRIDICT/PRIDICT2.0... predictive power is sometimes limited across different targets due to variations in chromatin context...

...we used different categories (Figure 2C). Note that in Figure 2, the numbers on the X axis refer to distribution...previous Addgene CRISPR publications.  References and resources References Pyhtila, B., Kasowitz, S., Leeson...through the years.  Libraries are divided into different categories based on function: knockout (KO), activate...have CRISPR plasmids deposited from labs in ~40 different countries, and we’ve shipped CRISPR plasmids to...

... Snapgene and Geneious software. References and resources References Chadeuf G, Ciron C, Moullier P, Salvetti...sequencing reads (~150 bp each) are aligned to the reference sequence of the plasmid used to create the viral...more than 90% of the reads should align to this reference sequence. Second, we perform a megaBLAST search...search on all the reads that did not map to the reference sequence. The resulting list of hits is then carefully...reads into longer DNA contigs without using a reference sequence. The five contigs with the most total...

... the protein within a living cell. Analyzing differences in protein localization and transport between...signals not visible to the human eye and are indifferent to subjective color intensity perception. Open...statistics that can be used to compare between different experimental setups. A colocalization analysis...one fluorescent signal in a channel setup for a different fluorescent signal) and photobleaching i.e. FP...molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities: Tracking intracellular...Markers of Subcellular Localization Poster here! References 1. Allen Institute Plasmid Pages 2. Cooper GM...

...but timer activity can be calibrated to these different conditions, enabling the use of a given timer ... of Neurog3 (a gene controlling pancreatic differentiation) in mouse embryos (Miyatsuka et al.) Distinguish... Let us know in the comments section below! References 1. Terskikh, Alexey, et al. ""Fluorescent timer...11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine...and Michael S. German. "Chronology of islet differentiation revealed by temporal cell labeling." Diabetes...programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-...

...chromoprotein and how is it different from fluorescent proteins? Before going into the difference between chromoproteins...research Read our Molecular Biology Reference Find plasmids for FRET References Blanquet RS, Phelan MA (1987...imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure 4). They created... Jennifer Tsang...

...rely on two-step hierarchical approaches using different Type IIS restriction enzyme specificities at each...Factors such as enzyme efficiency, stability, and buffer compatibility have placed practical limits on single...Assembly test systems Our research focused on three different levels of assembly: High efficiency and accurate...biotechnological cloning efforts for over 50 years, prefers ligation of Watson-Crick base pair substrates, ...products to market, New England Biolabs, Inc.       References 1. Vladimir, Potapov, et al. "Optimization of...cloning protocols Check out our molecular biology reference pages ...