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We narrowed to 811 results for: lars

Showing: 321 - 340 of 811 results

  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...themselves using computational approaches to analyze large data sets (myself included) and we are faced with... lost in the wide word of informatics. I am a molecular biologist who began coding for my thesis project...will keep you from repeating your work or making similar mistakes in the future. Stay organized Set up...for those that are just starting out, and helps clarify the role of the code. # This is a comment in python...student at University of Pennsylvania. She is particularly interested in science communication and epigenetics...

  2. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ... will provide you with an overview of general molecular biology knowledge and techniques. If you are interested...our screening overview blog post.) *Note: Two Popular Vectors for Blue White Screening Available at Addgene...of this gene, disrupting the lethality. This is similar to α-peptide DNA disruption in the blue-white screen...may contain genes that allow for the use of a particular substrate in defined medium, without which the...Resources on the Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated...

  3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...the past several years, it seems that the more polarized (negative) the action potential of a cell, the...differentiated and quiescent it is, and the more depolarized (positive) the cell is, the more embryonic, stem...cation channel) light-activated channels to hyperpolarize tumor cells, which resulted in tumor regression...activation, it stays open for a longer duration. Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin...and gap junction protein-mediated cues control polarity during planarian regeneration." Developmental ...

  4. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...talented and motivated groups and individuals with similar ideas to actually do something about it. Building...workshop. These workshops illustrate the open and modular nature of the workshop content: different parts...results, and tracking material downloads. This was particularly helpful for securing funding to support this...

  5. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...basics of molecular biology and cloning, since they have reasonable introductions to lots of molecular biology...quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr was the hottest phone...biology,” — Reddit commentor Yay4sean, in r/molecularbiology.   Oh, to be young again! Addgenie Marcy...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing...

  6. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...academic lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly... lab management software aren’t just limited to large volume facilities; it can be useful in academic ...you sad),  someone has put in a request for a particular plasmid and now it’s time for our lab technicians...this, it is important to write loosely coupled, modular code. Use tried-and-true tools We use Python, Django...

  7. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...these mutants are not toxic to HEK cells and that similar mutations can improve the specificity of Cas9 derived...from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog of choice...SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken sequence independent...found in eSpCas9, the effect on specificity was similar; SpCas9-HF1 generated fewer off-target cuts when...variant displays comparable on-target activity and similar or decreased off-target activity across the sites...

  8. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...as T-DDOG and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...activating gene expression. Although Cre isn’t a modular protein, Tang et. al were able to create a split...to many types of proteins. For proteins with a modular structure, constructing split variants should be... also possible to create split versions of non-modular proteins like Cre. Could Cas9-DOG be developed ...

  9. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ... the digital music revolution hit. I predict a similar revolution for antibodies. Recombinant antibody...that may not work well in animals because of similarities to host proteins. After the antibody of interest...long-term stability, consistency between batches, and molecular definition of recombinant antibodies are essential...US alone (Bradbury & Plückthun, 2015a). With molecularly defined antibodies that do not change over time..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are...

  10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...friendly, to own the process entirely remains a large undertaking. Priority number one was to ensure that... There are regions of some plasmids that are particularly difficult to sequence and assemble, including...regions that are GC rich. Since NGS assembles a larger sequence from many smaller sequences, regions with...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are...Viral preps and pooled libraries In addition to regular plasmid sequencing, many of you will be familiar...

  11. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...reproducibility of experiments. Inbred mice are particularly useful for understanding a specific effect related...that alters or eliminates the expression of a particular gene. Targeting vectors used to create knockout... a way to track successfully engineered cells. Similar to transgenic mice, knockout mice are engineered...off-target effects on regions of the genome with similar homology to the site of interest or create mosaic...recombination of floxed sites in the cell can occur. Similar systems include tetracycline or doxycycline mediated...

  12. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...perform more modern techniques used in genetics or molecular biology because the experiments are either not...templates which they can easily generate off of a circular plasmid via PCR. Typically, to make these constructs...complementary DNA (cDNA), and then use PCR and molecular cloning to insert the cDNA into the desired plasmid...those who are interested in performing their own molecular cloning and investigating genes not in this collection...vector backbone. The empty vector PLL4, which is similar to PR-T4P, is available from Addgene. If you use...

  13. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...the bait at a particular point in time, i.e. when the bait is interacting with a particular protein partner...specifically identify proteins that interact with a particular protein  complex as opposed to an individual ...partner or when the bait is part of a particular protein complex. Not performed in a native cell context...bait proteins stop interacting. This feature is particularly useful for in-depth characterization of dynamic...

  14. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... are your best bet to figure out how to use a particular reagent.  We also have links to later papers ...look! This stuff works!” These papers can be a particularly good resource if you are considering using a...serious science questions, consider using Google Scholar, which searches scientific articles, patents, and.... Addgene keeps a Document of Gratitude from particularly enthusiastic customers who have enjoyed their...combatitive. Not every question is answerable, particularly when it comes to technical questions. BUT, I...

  15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming...Szostak’s lab continues its work on the origin of cellular life. His lab has long hypothesized that life ...Nikolaus Rajewsky from the Max Delbruck Center for Molecular Medicine presented another unexpected finding ...regulatory RNA field. The Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These...abundant in neuronal synapses. shRNA silencing of one circular RNA they have identified resulted in a cell migration...

  16. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...Management skills”. The NPA states that Postdoctoral scholars should have the skills and techniques needed to... how to adapt to different situations and, in particular, to different styles of leadership. As the Chair...Committee would change format depending on the particular style and requirements of the new Board Members... more independently and some others would need regular re-direction and alignment with the overall mission...organization to develop these leadership skills in particular because every year it organized a “Committee ...

  17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...after receiving her degree she was faced with a similar conundrum – she had the skills, training, and motivation...our ideas got pretty good. We started doing extracurricular research on some of them to see how feasible...in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and...company, and that’s what we want it to do. It’s exhilarating to have that sort of freedom. It can also be...and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois ...

  18. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...also possess similar morphologies, growth properties, and express stem cell markers similar to ESCs [11...lentiviral infections respectively [9, 10]. These stellar works led to a Nobel Prize for Dr. Yamanaka in ...11].  While iPSCs and ESCs have similar functional utility, iPSCs bypass the transplantation compatibility...2. Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming...

  19. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ..., there may be particular poster you are interested in viewing which has drawn a large crowd, making it...don’t fully understand the question, ask for clarification. Having them reframe or elaborate their thought...posters you are interested in viewing. For most larger conferences, poster titles and abstracts are available...permitting, you can go back to the most interesting and popular posters at the end of the session. Get in contact...

  20. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...the conference you are attending on the larger side? At large conferences, chances are that several talks... the other person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch... contact information if your conversation is particularly fruitful. After the conference Bring back what...LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation, and you have their ...
Showing: 321 - 340 of 811 results