We narrowed to 394 results for: organization Addgene
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TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....the wide array of Cas9s (many available through Addgene’s repository) and make it easy to find your perfect...variety of species and cell types can be found on Addgene’s website. Activating target genes using dCas9-based...variety of species and cell types can be found on Addgene’s website. Fig. 3: Cas9 repressors include...What do i do next? Designing or selecting a sgRNA Addgene may carry a validated sgRNA for your target gene...Next CRISPR Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that...drosophila, worms and mammals (Browse plasmids by model organism, here). More information on the various delivery... ). 368, 290–296 (2020). More resources on the Addgene blog Read all the posts in our CRISPR 101 Series...
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Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog PostPublishedDec. 2, 2019, 2 p.m....suitable for organoid or in vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the...These newer tools are also currently available at Addgene. BioID2: A smaller improved promiscuous biotin...Efficient proximity labeling in living cells and organisms with TurboID." Nature biotechnology 36.9 (2018...PMCID: PMC5467174. Additional resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101...many fusion proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized that the... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively...al 2017). Find Additional CRISPR Resources on Addgene's CRISPR Guide Page 2. Optimizing "cut-to-mutation...PubMed PMID: 28102837. Additional Resources on the Addgene Blog Learn about High Fidelity Cas9 Variants How...Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed... (2017). PubMed. Additional Resources on the Addgene Blog Check out our CRISPR Featured Topic Page...CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....provide value to others This is a non-profit organization. Therefore, collaboration and community is the...Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental Designs Consulting... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide! Find CRISPR...Activation Mediators (SAM) system available from Addgene. This system uses the dCas9-VP64 fusion commonly...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo CRISPR delivery... Find SAM plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli...packaging plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S...PubMed PMID: 25716921. Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR ... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....partnered with Addgene to make our collection of integrin antibodies available through Addgene’s repository...10.1038/s41467-022-35173-w More resources from Addgene Addgene's Antibody Guide Antibodies 101: Flow Cytometry...extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...on these cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....indispensable tools for manipulating cells and organisms. They can be used for a variety of research purposes...vectors (for a more detailed explanation, check out Addgene's Lentiviral Guide). To produce lentiviral vectors...integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation... how to package lentiviral vectors, check out Addgene’s Lentiviral Guide and our lentiviral production...Central PMCID: PMC9377332 More resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle...every conversation! This blog post was written by Addgenie Rebecca Hutcheson. References and resources... to All Things Lentivirus More resources on addgene.org Lentiviral Guide Popular lentiviral vectors... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr...of genetic markers for your reference. Browse Addgene's curated list of Bacterial Expression Systems. ...blog post covering E. coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....this year! Find Addgene's own Lentiviral backbone plasmids here! Figure 2: Addgene cloned EGFP into...Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081), then generated...particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected...Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat...increased sensitivity and tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant...2025.03.20.643984. Build your way: Explore Addgene's new empty backbones By Meghan Rego You asked, ...EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these new backbones special:... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....of the Tet-Off system. For more information on Addgene Tet plasmids, see our Tetracycline resource page...has also been implemented in a variety of other organisms, such as sphingomonads and bacillus. ADH1 The...PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez, Alberto, Dominic Didiano...PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, Christopher J. Potter, Liqin Luo &...PMC3846601. Find plasmids from this publication at Addgene. Riabinina, et al. “Improved and expanded Q-system...PMC4344399. Find plasmids from this publication at Addgene. Riabinina O, Potter CJ. The Q-System: A Versatile...PubMed PMID: 22129153. Additional Resources on the Addgene Blog Quick guide to working with Drosophila Terminators... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...PMCID: PMC5837189. Additional Resources on the Addgene Blog Read our Introduction to GFP Read our Fluorescent...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...Fluorescent Proteins 101 eBook Resources on Addgene.org Browse the Fluorescent Protein Collection Find... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....s toolbox. Depending on the growth rate of the organism of interest, from the day primers arrive, the ...PubMed PMID: 24733238. Additional resources on the Addgene blog Read our Plasmids 101 blog posts Find our...about Lambda Red recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our ... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m.... abundance. If a scientist wants to know if an organism has a particular gene mutation, southern blotting...PubMed Central PMCID: PMC4411503. Resources on the Addgene Blog Learn More about Restriction Cloning Get ...Check out Plasmid Cloning by PCR Resources on Addgene.org Check out Our Protocol Pages Find Molecular ... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m....which allow AAVs to broadly spread throughout an organism, including difficult-to-target tissues like the...number to the capsid. Most vectors provided by Addgene are pseudotypes using the genomic structure of ...repository and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV1 tools are...repository and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV2 tools are...repository and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV5 tools are...service and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV8 tools are...2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors are not available through our Packaged... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m.... transposons are delivered to cells or a model organism, they insert into the host’s genome. Then, the....3390/ijms22105084 Additional resources on the Addgene blog Learn about the INTEGRATE system for bacterial... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....impact of CRISPR editing in the model cell line or organism. Several options exist for unbiased off-target...PubMed PMID: 27494557. Additional Resources on the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide...Models with CRISPR/Cas9 Additional Resources on Addgene.org View Our CRISPR Guide Pages Find CRISPR Plasmids... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...different in nature (eGFP being a protein and FITC an organic molecule), their bleed is so strong that these ...sailing, and reading. More resources on the Addgene blog Antibodies 101: Introduction to Gating in ... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m.... and do not necessarily represent the views of Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan...I was also incredibly impressed with how well organized the march was, as well as the friendly atmosphere... and @SciCATSyvr. Additional Resources on the Addgene Blog Read Our Previow Post on the March for Science... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....fluorescent dyes have enabled the visualization of both organic tissues and cell cultures, opening biological interiors....1016/j.bbamem.2013.09.005 More resources from Addgene Fluorescent protein plasmids and resourcesA practical...cellular components. Some dyes target specific organelles like mitochondria, and different dyes can result...