We narrowed to 440 results for: pes;
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TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....drastically alter your strategy. I realized that similar types of thinking go into developing gene therapy strategies...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....sequencing of the genomic DNA. However, some cell types are poorly transduced by lentivirus, and thus screening... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m.... into two broad categories and thus, causes two types of editing outcomes: Cas9 breaks repaired by the... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....out what works for you for each of your meeting types (see above). We’ve provided some tips above for... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....following flow chart to get you thinking about the types of experiments you're planning on doing and read... -
Advice for Scientists Starting a Lab
TypeBlog PostPublishedApril 29, 2014, 1:43 p.m....graduate student or postdoc may require different types of mentoring. Some mentees prefer lots of advice... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....flexibility allows it to be used with different types of PCR-generated inserts. By harnessing the power... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m.... genes on the plasmid can give them different phenotypes. For example, this plasmid encodes a green fluorescent... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....later on. Enhancer and suppressor screens These types of screens are very similar to forward and reverse... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m.... with finding the best system to document these types of analyses and their results. We are adept at recording... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....Megaprimer PCR Gibson Assembly GeneArt Cloning Types of Mutations Insertions, deletions, point mutations... -
Plasmids 101: Blue-white Screening
TypeBlog PostPublishedJune 4, 2015, 1:03 p.m.... have your insert (blue-white screening). Other types of screening methods Although blue-white screening... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....DNA synthesis. NHEJ also finishes repair of most types of breaks in tens of minutes – an order of magnitude... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....to apply the new library for use in various cell types and in live mice. Therapeutic uses in humans are... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....product in action) Can help you learn the local “ropes” and become an insider faster A mentor that works... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....to several alternative affinity reagents. Types of Affinity Reagents (Listed in no particular order... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m.... was raised in different species or are unique isotypes. If every antibody in the assay is a unique isotype... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....future experiments. Being able to work through these types of bigger picture problems systematically and logically... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....TRIM-away to nine proteins in ten ddifferent cell types, including difficult-to-manipulate primary cells...