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...resources on self-promotion (how hard it is for some people, especially women), visibility (how to get it, ...especially if introverted ), and networking (how to get people to remember you). What better way to accomplish...Interviews: The All-Important Job Talk. 1. Watch other people give great talks We are not talking about lab ...simplicity of their slides and how the slides complement the spoken message. Learn from their level of...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...on a slide but not reading the explanation. The people that need the information will find it. Experienced...particular question. I like to ask about how many people use plasmids in their research and then see how...

...Institute for Biological Studies) Multiple uses: From multiplexing to multiscaling NIR-shifted spectrum...spectrum is perfect for use in spectral multiplexing (Shcherbakova et al., 2018). NIR FPs can be combined ...Verkhusha, V.V. Near-Infrared Fluorescent Proteins: Multiplexing and Optogenetics across Scales. Trends Biotechnol...11, 239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest...cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, 279 (2019). Oliinyk, O.S. et...Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and optogenetics of Rho GTPases enabled...

...Brainbow multicolor labeling system - a perfect example of the continued utility of Cre-lox. Check out ...events specified by three loxP variants, but the principles remain the same. The first fluorescent protein...than 3-4 colors due to the tandem integration of multiple copies of the construct (about 8 in Brainbow-1.0...-1.0 mice.) The combinatorial effect of multiple, independent recombination events leads to a rainbow ...Brainbow-2 each contained a “default” state; for example, Brainbow-1.0 expresses RFP when the construct ...system, Sanes and Lichtman also developed a complementary Flpbow construct that is functionally similar...13):2835-46. doi: 10.1242/dev.094631. PubMed. Multiplex cell and lineage tracking with combinatorial labels...

...DePace’s yearly planning meetings revolve around two simple worksheets, each of which is filled out separately...summarized briefly below (and available of in the supplement of this paper), help each person organize his...including major project goals and effort needed to complete them. These documents dictate the subject matter...concrete plan in hand, trainees are motivated to complete their work rather than overwhelmed or confused...meetings may not look the same in every lab, but the principles behind this technique are beneficial for researchers...Extracurricular Activities for a Strong Science Career Path 7 Simple Strategies to Resolve Conflict With Difficult Supervisors...

... and a mashed potato bar (complete with all the fixings), as well as plenty of delicious desserts. We ... 10-year Anniversary Gala celebrated all of the people who have helped Addgene grow and spread our mission...playing all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you might have...Addgene over the last 10 years as well as the many people that make up the Addgene family. It was great to... it to fruition. The concept, though relatively simple, changed the course of scientific research. What...

...sensors Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT, with AAV vectors are common...vectors, and post-translational regulatory elements. Examples of these approaches are given in Tables 3-7. 3...specificity of cell targeting even further. For example, injecting AAVs at one location can result in expression...that received information from a region. In the example shown in Figure 1, injected retrograde AAVs at ...neuroscience is becoming more and more versatile. To complement the wealth of genetically encoded tools available...only reagents, but also method information. To complement these efforts, we’re beginning to share data ...

...Scientists are excited by somewhat unusual things. For example, I am not that interested in meeting movie stars...astronaut Jim Lovell I was speechless (the club of people who have been in space is pretty small). Therefore...this over 30 minutes, which was fun to watch. A simpler 1 protein - 1 RNA system Drs. Dounda and Charpentier...into one RNA to direct cleavage the result was a simpler 1 protein - 1 RNA system.  Dr. Charpentier advocated...breakthrough. In fact, the panelists agreed that, for example, the technology is already available today to cure... anemia in stem cells but this can’t be used in people until we learn more about safety and more about...

...that plagues other red GECIs, so the team could multiplex PinkyCaMP alongside green fluorescent and optogenetic...an inverted topology of FRCaMP (itself based on mApple, which means it likely has the blue-light photoswitching... exceptional brightness, photostability, and multiplexing capabilities. bioRxiv 2024.12.16.628673. https...Biomolecular condensates are membraneless liquid "droplets" that can concentrate and sequester specific macromolecules...extensive validation of the cell and gene therapy, so please check it out! Find AAV-CRISPRa plasmids here! ... the Qi lab's website crisprkit.org to request complete kits or find protocols and other resources. Find...  Multiple Authors...

...engineered ESCs), and chimeric adults mated to produce completely transgenic offspring. Although powerful, this...about 100 bps into the run, it got “dirty”, with multiple sequence traces overlapping each other! When the... finally, we have a technology, CRISPR, that is simple in concept, straightforward to use, and robust ...missing or added at the broken ends and, as such, cripple the gene. However, if repair material is provided...enrichment and selection offered by mouse ESCs. For example, we often screen only 15-25 mice when generating... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering...

... or condition of interest using a simple keyword search. For example, searching the term "breast cancer...results section, but you had to dig through the supplemental files to see the actual data. Now you’re wondering...datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it possible for...sgRNA-level sequence read scores. The original supplemental data tables available with the publication are...broadest possible scope of screens to be curated, for example phenotype screens with read-outs other than cell...

...built, literally, 'brick-by-brick' to introduce people all around the world to the lives of researchers...wrong. Whether it be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments...Unfortunately, unless they’ve worked in a lab, most people don’t get to experience this very human side to... is to help make science mainstream and to show people the very human struggles in the laboratory routine...sharing and laughing at these struggles will bring people together and make these daily challenges a little...that we step on them with bare feet), to connect people with science. And so, “Bricking Science” was born...

...can gauge if people are happy depositors is if they deposit plasmids more than once. People get hooked ...Flame Awards. What's in a flame?     We’re always pleased when scientists around the world choose to share...Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute our plasmids via Addgene. Given the... plasmids and have not yet received your award, please contact [email protected] to claim your Blue Flame...deposit. Begin yours here. It has been my genuine pleasure to send these small tokens of thanks for the incredible...

... had to get used to the workspaces, as well as complete the lab safety and cybersecurity training before...project is a major project given to each intern to complete and present on.    Figure 1: Addgene's...weeks to work on cloning the Syntaxin3 gene in a pLenti vector backbone. During those five weeks, I worked...obtain eight clones. We ran digested and undigested samples of the clones on an electrophoresis gel in order...a 100% successful clone of the Syntaxin3 in the pLenti backbone! Once everything was confirmed, I worked...Toj-Villagran is a rising senior in high school who just completed their summer internship at Addgene. They plan ...

... detection methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL) and subject...this resource for the community. There are a few simple steps you can take to input your data for sharing...and assessment of your experimental results. For example, if you visualized expression after AAV injection...generated with a caption. For microscopy images, please include channel imaged, a color legend for probes...clear overview of what you need to submit data. Please note that the data hub is currently still in beta...

...say that most people do not recognize its importance or perhaps even know it exists. Couple that with a ...in Columbus, Ohio, ABRC ships more than 100,000 samples to researchers and educators in 60 countries each... resources, we seek out opportunities to engage people in science learning. Whether visiting classrooms... 2017 alone, ABRC staff reached more than 4,000 people through our outreach efforts. Our education partnerships...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants...

..."CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv (2019)... be tagged with different CellTag libraries at multiple timepoints during reprogramming. These library-specific...library-specific identifiers act like a timestamp and help demultiplex the scRNA-seq data and build multi-generational...phenotype typically emerges. Every three days, a sample of cells was collected for scRNA-seq analysis and...tended to have similar reprogramming outcomes. For example, cells with shared CellTagD13 tags often had similar...

...until you don't, of trying to understand your completely unexpected findings, or understanding why your...thrice-failed experiment, this paper was actually from a completely unexpected finding in a different experiment,...your conflict, you’ll need two other things to complete your story: an introduction, and a resolution.... big-picture problem, by asking your audience a simple question, like “Have you ever thought about how...conflict to get your audience invested in the more complex story, and then build on that in your resolution...journey, stories from patients or other affected people, man/animal vs nature, anthropomorphized animals...

...educational materials to our repository of open samples. We are, and have always been, a community resource... to maximize the reach and impact of your work. Please email [email protected] if you would like to ...ideas for new materials to add to the collection, please let us know by filling out this suggestion form... will find useful antibodies for your research. Please consider sharing your experiences with antibodies...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8:e43322. https....43322 Bandrowski, AE, Martone, ME (2016) RRIDs: A Simple Step toward Improving Reproducibility through Rigor...

...would rather fund excellent people with mediocre ideas than mediocre people with excellent ideas. I am ... mentors -- well, it's time you call upon these people for guidance.  Before even beginning the process...this part might hurt, but if you pitch to enough people, you will find the answer to your viability question...asking to meet. I have cold emailed hundreds of people asking for meetings to discuss miLab.  Although...impressions are everything and you want to leave people remembering your potential as an entrepreneur, ...

... in principle adapting a FRET sensor for monitoring calcium versus zinc should require the simple swapping...dozens of other published FRET-based sensors. For example, his team is now applying it to improve upon an...domains, in practice optimizing the sensors involves plenty of trial and error. Transition metals also present... of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx said. “For us, it... developed something useful [enough] that other people start using it. For us, it’s like a test.” References...