We narrowed to 793 results for: por

...length can and should be varied as it can have important implications for the fusion protein effectivity...upon these original tools that allows for both spatiotemporal protein degradation and quantification of this...et al., 2021.   The AID system relies on a transport inhibitor response 1 (TIR1) expressed under a ...a desired promoter with a specific spatiotemporal expression pattern and an auxin inducible degron (AID...

...difficult to genetically edit due to toxicity from electroporation or poor cellular entry with other transfection...PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist peptide.... CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons, with...slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized by pre-existing antibodies...

...sensors are better classified by their fluorescent reporter and the mechanism of their voltage sensing component...rhodopsin proteins were initially evolved for ion transport, not fluorescence, they are not nearly as bright...for penetrating deeper tissues depths (1 mm), important for certain applications such as in vivo tissue...rhodopsin Ace2N and mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET.  Image from ...

....com.   The Cascade complex Cascade is an important component of the CRISPR Cas3 system. Most Cascade... appears to be conserved. This DNA degradation portion can be bypassed by leaving out Cas3 — a tactic ...is pulled through the Cas3. This mechanism is important for Cas3’s biggest trick. Cas3 likes to bluff,... — Cas3 will definitively degrade the targeted portion of DNA using only a single gRNA. Cas3 appears to...

...this rarely happens. But if you ever have an opportunity to read one of these structure papers – while...function in the human body?” … and probably most importantly… "how do we know this?” Over the past fifteen... nuclease active site wasn’t even close to its reported cleavage site on the complementary DNA strand....model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-...

...in-person portion got canceled, taking advantage of virtual meetings can still be a great opportunity to introduce...course of the last few years: recognizing the importance and benefit of remote and video communication...staff have become a new way to allow you to get important information about the campus even if you can’t...

...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably... your experimental setup less cumbersome. 3. Portability across multiple systems Terskikh et al. showed...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology...Chronology of islet differentiation revealed by temporal cell labeling." Diabetes 58.8 (2009): 1863-1868...

...suffers from several limitations. Perhaps most importantly, most Cas9 orthologs are less well characterized...(1, 3). The gRNA can be further modified by incorporation of an RNA hairpin, such as the MS2 hairpin, ...with nuclease-competent Cas9. An RNA hairpin incorporated into the truncated gRNA recruits a transcriptional...target site. Benefits of truncated gRNAs Importantly, the use of truncated guides has largely been...

...has made strong efforts in the last 5 years to incorporate more science and technology into the school curriculum...presentation to their students. I didn’t think of this opportunity as coming from networking at the time, but that...presentation because, in my opinion, it was more important to communicate the applications of CRISPR over...use it? This last section was arguably the most important, and it was a little worrisome for me since bioethics...

...expression plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific...including Protein A, 1xFLAG, 2xAM, and HA have been reported, as well as affinity purification with an anti-Cas9...this technique, researchers have begun to make important discoveries in various biological fields. In 2015...chromatin immunoprecipitation (enChIP)." Scientific reports 3 (2013): 3171. PubMed PMID: 24201379. PubMed Central...

...toward saying that host-virus interactions are an important part of the biology of adenoviruses (AdVs). AdVs... Although host-virus interactions likely seem important for any virus, we will see that they are very ...(Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation of...Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org...

...analyzable transgenic in hand, so it is pretty important to get this process right the first time. I’ll...to transform when they begin flowering. It is important to perform the transformation not long after flowering...cut off the primary inflorescence (the flowering portion of the plant) near the base of the stem. This buys...s time to select for transformants. It’s very important to select multiple T1 plants from each transformation...

...typically require an exact DNA sequence to be incorporated at a precise genomic location, with little to...template for repair, which will result in the incorporation of your sequence, a.k.a a knock-in! So how does...higher HR frequencies than quiescent cells. It is important to know that HR is often mutated in cancer cells...molecule is used as the template, you need to incorporate homologous sequence to the right and/or left ...

...transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure and then used with ...attach a fluorophore at the specific site of incorporation (Figure 1d). Fluorogen activating proteins...excess dye from the sample, which is particularly important for samples where washing is difficult, such as...inside living cells. We recently discovered that incorporation of four-membered azetidine rings could substantially...

..., as well as the Editorial Board of Scientific Reports and PLoS One. Additionally, you are involved in...is being involved in the scientific community important to you and your lab? Fujii: I first knew Addgene...interact with each other, which is critically important, especially for young researchers. It was a really...technologies. Majority of them are eager to just "import" technologies developed abroad, so it's a little...

...Geneva (Creed et al., 2015) used optogenetics to support these existing findings by controlling neurons ... animal is placed into a tank of water with no support from below or anything to hold on to. In general..., 2007). A study from 2014 (Bahi et al., 2014) supports the relevance of BDNF in depression. The researchers...of viral vectors to fine tune gene expression temporally and spatially, exciting times are ahead for behavioral...

...number of systems that can be studied by providing support "for development of tools, approaches, and infrastructure...great for studying parenting strategies and make important ecological indicators since species live in water...Aiptasia - algae Breviolum minutum symbionts are important models for solutions to the coral reef crisis.... interesting biologically. They have economic importance as harmful weeds. The parasitic fusion is such...

... editing efficiencies in vitro and in vivo. Incorporating reverse transcriptase (RT) variants, PE6a and...., 2023, reused under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on... more. For added precision, the authors also incorporated LOV-Turbo into TransitID experiments.  Find ...in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells...

...synthesis polymerase known to preferentially incorporate A bases across from abasic sites. This approach...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse...a template — in this case, encouraging it to incorporate a new base across from the abasic site before...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids...

...2024). They developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7...high microglia-specific gene expression when incorporated in an AAV vector (Figure 5). Efficiency and ...including in human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous...associated with viral vector-based therapies. REVeRT supports advanced gene expression regulation, enhancing...