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Showing: 321 - 340 of 521 results
  1. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...difficult to use in a multicolor/multichannel imaging set-up. In contrast, smFP tags can be recognized by distinct...
  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... used successfully in preclinical and clinical settings for ocular conditions. However, AdV was abandoned...responsible for an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...
  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...
  4. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning...sequences. Intermediate chaining products (called “cassettes”) can be readily reused in the assembly of any...any later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used...
  5. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    .... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...
  6. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...independent variable. Biological replicates In any given set of experiments, the biological controls are more ...
  7. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...
  8. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...it’s a combination of many factors unique to this set of tools and the community they serve. As difficult...validating and using an AAV in a specific experimental setup. Teams across Addgene have worked hard to ensure...compared to other AAVs preps from 2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and...
  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...your “gating strategy.” This series of markers are set up sequentially, from broadest to most specific marker...Subtypes of immune cells are often defined by a subset of the markers used to identify them, such as “...
  10. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ... document examples are not especially helpful. I set out to make something practical and accessible. If...
  11. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...gene-targeting capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene...genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777...
Showing: 321 - 340 of 521 results