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...and/or perceived professional risk involved in setting up a CURE, especially if it’s a new approach within... amenable to in-class labs. Provost ultimately settled on a two-semester model, in which students learn...

...nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

...This modular approach combines an interchangeable set of fusogenic and targeting components to reprogram...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

...have become a powerful tool to address a diverse set of biological inquiries. References Ogura, Teru...death. The team used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed...for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be ... selects for those cells that have removed the cassette through a second recombination event between the...

...discovered in 1856 by William Perkin. His discovery set off a flurry of activity and the majority of the ...

.... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...

...example, with standard fluorescent microscope filter sets, users can label multiple proteins of interest with...

...Fridays. From each overnight culture, we prepare three sets of 2D barcoded tubes. Two of the tubes are used ...

...independent variable. Biological replicates In any given set of experiments, the biological controls are more ...

...multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

...team members only, under the specific permissions set for every member of your team. Do you need to make...

...in the best-case scenario [7]. In the clinical setting where patient blood samples are limited, such low...by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back...Mukherjee is currently a postdoctoral fellow at Massachusetts General Hospital with research interests in ...

... (Cong et al, Mai et al) we were very excited to set up a CRISPR-based editing platform in our lab. Our...

...frogs, mice, and even humans. It binds to a specific set of DNA-binding transcription factors to repress the...

...gene-targeting capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene...genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777...

...it’s a combination of many factors unique to this set of tools and the community they serve. As difficult...validating and using an AAV in a specific experimental setup. Teams across Addgene have worked hard to ensure...compared to other AAVs preps from 2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and...

... document examples are not especially helpful. I set out to make something practical and accessible. If...

...If your lab owns a liquid handling robot, you can set it up to automate the reagent transfer and the restriction–ligation...

...your “gating strategy.” This series of markers are set up sequentially, from broadest to most specific marker...Subtypes of immune cells are often defined by a subset of the markers used to identify them, such as “...

...forums on particular topics. Volunteer moderators set the rules for these forums and try to keep discussions...