We narrowed to 457 results for: val

1. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...Cas12a. Image from DeWeirdt, et al. 2021 To validate this optimized genetic screening technique, researchers...

2. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   Published

   June 29, 2021, 1:15 p.m.

   ...Because of the steps involved in producing and validating monoclonal antibodies, their cost is higher than...

3. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ...easily sort them by other parameters, such as E-value for protein sequence searches. Additionally, the...

4. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...provide resources, knowledge, and most importantly, validated protocols (using readily available reagents!) ...

5. Viral Vectors 101: Types of viruses

   Type

   Blog Post

   Published

   June 6, 2023, 1:15 p.m.

   ...evolutionarily optimized gene delivery devices. Their survival depends on their ability to deliver and permanently...

6. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   Published

   April 27, 2023, 1:15 p.m.

   ...interpretation of results. Take extra care when evaluating how this serotype works in your system. Chemogenetic...

7. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ...for analyzing time-lapse images, quantitatively evaluating antibody staining, or counting cells are now ...

8. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and activity (Knudsen et al...

9. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ...hypothesis testing statistical tools that generate a p-value.  As an example, if you hypothesize that growth ...

10. Behind the scenes: Addgene’s new search engine and more

    Type

    Blog Post

    Published

    Dec. 12, 2019, 2:22 p.m.

    ...term that appear in our data into a single filter value. For example, there is only one viral prep filter...

11. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...Gryder. Addgene depositors or not, your time is valuable ...

12. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...inducible pLac promoter. This promoter requires removal of the lac repressor (lacI protein) for transcription...

13. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...project. README: The README.txt document is invaluable. In the top directory of every experiment, immediately...

14. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

15. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...phosphorylation status of MEK1/2 was tracked at 5 minute intervals via Western blot. Phosphorylation of MEK1/2 peaks...

16. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...contributed by Neil J. McKenna and Bert W. O’Malley. Survival of all organisms depends on efficient energy maintenance...

17. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    Published

    Feb. 11, 2014, 12:13 p.m.

    ...challenging because it’s hard to determine the exact value of costs. It is important to be aware of supply ...

18. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    Published

    Jan. 29, 2015, 1:25 p.m.

    ...under-represented minorities. Her online content is of great value. I have never been in contact with Aida Baida Gil...

19. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...will be disrupted or deleted. Selection marker removal – in conventional mouse targeting, targeted clones...

20. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...targets ~2 Mbp apart, they noticed that they could evaluate the degree of chromatin compaction even for this...