We narrowed to 783 results for: INA

...Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans DNA editing with STITCHR...tagging: Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin resistance...11012), and counterstained with DAPI. New recombinant antibodies targeting Glypicans By Ashley Waldron...) recently released a set of highly-specific recombinant antibodies against three of the six human glypican... et al., 2024). While dCas9 has been used in combination with proximity labeling enzymes before, such ..., Bosch, J. A., Sanchez, A. D., Udeshi, N. D., Svinkina, T., Carr, S. A., Feldman, J. L., Perrimon, N....parvalbumin (PV), somatostatin (SST), vasoactive intestinal peptide (VIP), or lysosomal-associated membrane...

...you are studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an...group of mice, the experimental group, with the new kinase inhibitor and a second group, the positive control...control group, with an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You ...experimental protocol but does not receive either protein kinase inhibitor. Since the negative control group does...variability. If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better...protocol and try to find a way to reduce, if not eliminate, the noise. This can be done either by modifying...labeling occurs (Burry 2011). Complex experiments Finally, additional controls may be required for more complex...

...expression.  Finally, the risks of potential toxicity and/or off-target effects (e.g. recombinase-dependent...rather than relying on other systems as proxies.  Finally, the size and receptor interactions of a serotype...Nonhuman Primates after Intramuscular Injection of Recombinant Adeno-Associated Virus. Journal of Virology, ...promoter results in robust transduction in the rat spinal cord following injury. Gene Therapy, 26(5), Article...adeno-associated viral vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy...doi.org/10.5607/en20006 Rolland, A.-S., Kareva, T., Yarygina, O., Kholodilov, N., & Burke, R. E. (2016). Expression...

...Like everyone else in the world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember...the day, March 16, 2014, that I got my first preliminary results from a CRISPR genome editing experiment...was shared by many others around the world. Yes, finally, we have a technology, CRISPR, that is simple in...conventional gene targeting, the cost may be $50,000. Finally, compared to conventional methods of genome engineering...advice from John Doench’s gRNA design blog post. Finally, always remember that you are working with RNAs... active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nat Biotechnol 32:1262–1267 . https://doi.org...in vivo genome editing in mouse embryos using recombinant adeno-associated viruses. Nat Commun 9: . https...

...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV2 Recombinase James M. Wilson New serotype pENN.AAV.hSyn.Cre.WPRE.hGH AAV2 Recombinase James M...AAVrg Recombinase James M. Wilson New serotype pENN.AAV.CamKII 0.4.Cre.SV40 AAVrg Recombinase James ...Ofer Yizhar New serotype pAAV-syn-Flpo AAVrg Recombinase Ian Wickersham New viral prep pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40...-iCre(R297T)-BGHpA (Alias: CN4825) AAV-PHPeB Recombinase Jonathan Ting New viral prep AiP15140: pAAV-AiE0873m...

...multicolor labeling is a powerful solution for discriminating between neurons for light microscopy-based ... introduced, stochasticity will result in a combinatorial expression of these three genes with different...inherently low. This is because the stochastic and combinatorial expression of fluorescent proteins is only possible...overcome the limited expression levels of XFPs in the original method, we used the Tetracycline trans-activator... long, as too much XFP is toxic to the cells. Inadequate expression levels of XFPs - Reduce the amount...Livet, Jean, et al. "Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...

...CRISPR/Cas9 gene edit. I wanted to inactivate the gene BRAF (a kinase implicated in several human cancers... really need to use FACS (like they did in the original publication). I thought I could see GFP expression...against GFP expression, but I didn’t. 2. In the original publication they see GFP expression in about 20%... cells at an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to...human line, using methods based on homologous recombination (Rago et al., 2007). Using CRISPR, I was able... active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench JG, Hartenian E, Graham DB, Tothova ...

...protocols, including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor...can reliably detect single-base differences. The original creators of this technique visualized the ligation... have been generally found to have a higher discrimination at the upstream side of the ligation junction...including Taq DNA ligase, are naturally able to discriminate against ligating substrates containing base ... at elevated temperatures, allowing further discrimination by incubating the ligation at a temperature... fidelity ligation can be achieved through a combination of the intrinsic selectivity of the ligase active...method allowed screening of all possible base combinations (Watson-Crick and mismatched) around the ligation...

... are two types of Class 1 TEs: those with long terminal repeats (LTRs) and those without (non-LTR TEs)...contain two open reading frames (ORFs) which often terminate with a poly(A). ORF2 encodes endonuclease and ...transposase enzyme binds the transposon’s long terminal repeats (LTRs), induces a double-stranded break...synthetic transposable element developed from inactivated Tc1/mariner transposons found in fish (Sandoval-Villegas...it leaves behind the CAG DNA footprint from its terminal sequences at the excision site after cleavage ...medaka fish because its insertion into the fish’s tyrosinase gene caused albinism (Koga et al., 1996). Unlike...Tol2 or miniTol2 is a truncated version of the original Tol2 and has ~3-fold increase in transposition...

...inventory of recombinases and recombinase-dependent vectors. We recently released several recombinases in various...Calcium sensors and GABA sensors Optogenetics Recombinases And more! Control AAV For many AAV experiments...densely-labeled neurons because background fluorescence from inactive neurons is reduced. This variant has an approximately...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV Targeting and manipulating specific, genetically-defined...