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We narrowed to 821 results for: LAS

Showing: 171 - 180 of 821 results
  1. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan; 339 (6121): 819–23. ...distinct types and subtypes using polythetic classification based on evolutionary similarity (5). Type ... in the field, and enabling access to multiple plasmids used for various platform applications. For more... out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or ...
  2. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte...was working in Voigt's lab. Years later, these classic systems continue to be optimized by Tabor’s lab...light-responsive system is spread across three plasmids, with the response regulator encoded in the genome...et al. condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few...These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness...constructs have been greatly streamlined; only two plasmids are required per system. Applications of these...many strains of E. coli, as well as with other plasmid-based systems, which will enable the assembly of...
  3. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...Control of Gene Repression and Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead...Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind...tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which expresses the activation helper protein. ...
  4. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...never been in contact with Aida Baida Gil of Coach De La Professional but the website looks interesting and...out Our Career Page for Recent Job Openings Find Plasmids to Expedite Your Research   ...
  5. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...Gaudelli NM, Lam DK, Rees HA, Solá-Esteves NM, Barrera LA, Born DA, Edwards A, Gehrke JM, Lee S-J, Liquori ...𝛽-globin), which cause sickle cell disease and 𝛽-thalassemia, can be mitigated with the expression of fetal...effects. ABEs can be introduced into cells as a plasmid or as mRNA constructs. In primary human cells mRNA... basics of CRISPR in our CRISPR guide Find all plasmids for base editing in our collection Sign up to ...
  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation...bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 468:67–71 . https://doi.org/10.1038...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids ...
  7. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...HC, Durbin AF, Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira...reagents and equipment has become a bottleneck. In the last few years, scientists have utilized CRISPR-Cas9 ...results in as little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection...sensitivity from patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR...Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Molecular Cell 60:385–397 ...) Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell 163:759–771 . https://...
  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning...the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in ..., the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected...microinjected into either the pronucleus or the cytoplasm of fertilized mouse eggs. Or, to avoid handling embryos...provided (in the form of an oligonucleotide or plasmid), precise changes can be made in the genome via...
  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...’Académie des Sciences - Series III - Sciences de la Vie 323:1053–1060 . https://doi.org/10.1016/s0764... our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing References ...this movie Ethan Hawke’s character revolts against class divide that is created by genetics.    Zinc finger...even insert new DNA at the target genomic loci.  A last word on the depiction of genome engineering in pop...
Showing: 171 - 180 of 821 results