We narrowed to 884 results for: ica

...conjugates (especially if you start to consider clinical applications). But there are a few other classes that... mechanisms. Most take advantage of chemical groups intrinsically present on antibodies, such as the primary...post-translationally. 4) Modifications made to antibodies, such as inclusion of non-canonical amino acids or peptide...Chemiluminescence, which is light produced by a chemical reaction, can be detected using specialized imaging...reactions produce a color change, so they are typically used in microscopy-based assays like immunohistochemistry...These properties allow for efficient signal amplification, making biotin conjugated antibodies good options...sensitive detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

...Much of today's biological research requires a close examination of specific proteins within a system...system. This can be pretty complicated given that a single cell has tens of thousands of proteins functioning...single protein? Oftentimes, they rely on protein purification. In this strategy a single protein can be isolated...this blog will focus on one of the most common, purification based on the polyhistidine tag. What is the ...purity (Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on ... incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order to ...2000).  One downside of polyhistidine tag-based purification, however, is the propensity for nonspecific ...

...resistance cassette, and the R6K origin of replication to allow replication only in hosts carrying the bacteriophage...Belarusian State University Series of Physiological, Biochemical and Molecular Biology Sciences. 9.1 (...Lambda Red recombineering, which uses enzymes domesticated from the Lambda bacteriophage, is arguably the...enzymes can also accomplish the desired genomic modification. This technique is frequently called allelic...recipient of interest. Because pDS132 cannot be replicated in the recipient, the only antibiotic resistant...They hypothesized that genes that had evolved specifically for toxicity would allow stronger counter-selection...exchange. To do this, we performed the following modifications to pDS132 (Philippe et al., 2004): Replaced...

... brainstorming new technological innovations. Let’s take a look at three historical techniques: southern...computers, cell phones, and cars become more technologically advanced leaving earlier versions obsolete,...that save both time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...cloning or knockout experiments to forensics and clinical diagnostics. One example of southern blotting being...interest by recording DNA abundance throughout the amplification process, rather than just at the end as in standard...fragments of a DNA sequence of interest to create a physical map of the DNA. Starting in the 1970s, restriction...

...UCIBIO) focused on understanding the physiological and pathological roles of human cell glycans. Accordingly... biosynthetic pathway and have a wide range of clinical manifestations. PMM2-CDG is the most frequent ...reported that PMM2-CDG patients suffer from immunological defects. These are characterized by a predisposition...these lines to study how immune processes and biological pathways are altered in PMM2-CDG. These studies...reducing their risks for infection and other pathological conditions. References: Monticelli, M., Ferro... J., dos Reis Ferreira, V. & Videira, P. A. Immunological aspects of congenital disorders of glycosylation...Biology at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA. The primary focus...

...of the FRET pair. Since many biological processes occur within the typical FRET range, FRET efficiency ...nanometers of each other, or measure the tension in the helical structure of spider silk, or the activity of a ...Fluorescence Resonance Energy Transfer. Unlike the typical excitation and emission of an excited fluorophore...donor fluorophore to the acceptor fluorophore. The typical steps in FRET are: 1. Donor fluorophore excitation...decision easier. 1. Müller, Sara M., et al. "Quantification of Förster resonance energy transfer by monitoring...Experiments using intermolecular FRET probes are typically used for studying protein-protein interactions...with an intermolecular probe is often either impractical (for biomolecules that are not proteins) or would...

...region you wish to stimulate 2) Fabrication and implantation of the optical fiber into the region of interest...run it? And, most importantly, what are the methodological and experimental limitations and considerations...numerous scientists at NIDA and some of the practicalities and considerations you may not find in the ...there of, depending on the opsin you use); the efficacy of optogenetics has already been proven. Therefore...region and cell type of interest is obviously of critical importance to any optogenetic experiment. There...pioneered the development of optogenetics, has genetically manipulated a number of the opsin genes to alter...optogenetics. Successful stereotaxic surgery relies on identification of the bregma and lambda sutures and subsequent...

...supporters of science communication. To help you think about ways to effectively communicate your science, we...Scientist Jessica Welch Have Had to Explain Importance of Narrative in Science Communication Conversation...Conversation in Science Communication The Purpose of Science Communication #1 Mistake Scientists Make when Communicating...News at Harvard University. Effective science communication is required to promote public support for research... organization that works to promote science communication. Science in the News hosts a podcast, seminars...That’s right, #ActualLivingScientists, eager to communicate their science. In this first half of our two-...programs SITN runs and learn how they think about communicating science. Listen to the interview with Science...

...various time points. Measuring the optical density of the culture can indicate the number of cells present in...in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than enough DNA to...are, you’ll even have DNA left over for other applications, like PCR, cloning, transfection, or long-term...wash step or use a different strain. For most applications, Addgene uses and recommends DH5𝝰! This strain...cloning Bacterial strains adapted for protein purification use the cell’s resources to produce high levels...retransforming the plasmid into a strain meant for DNA purification. It sounds like extra work, but it will likely...your E. coli to ensure it is suitable for your application. We have previously reviewed many common lab ...

...light and chemicals respectively. Didem Goz Aytruk from Connie Cepko’s Lab at Harvard Medical School describes...although fluorescent proteins are incredibly useful, chemical fluorophores can still be used in a wide variety...variety of experiments. Read more to find chemical fluorophores for your next experiment. Nucleic acid based...systems that report the order in which specific chemical inputs are received by a cell. These so-called...report upon and/or engineer “temporally ordered biochemical events” such as those that occur during development...containing only the sequences required for viral replication and transcription as well as reporter genes. ...Membrane-Rupturing Microscopic Tools In this post, Jessica Polka, formerly a postdoctoral researcher in the...