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We narrowed to 792 results for: LAT

Showing: 511 - 540 of 792 results

  1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...expression cassettes on a single plasmid (Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning,...Fluorescence Complementation (rBiFC, Grefen and Blatt, 2012). The 2in1 Plasmid Toolkit consists of 4 plasmid...Biol, 2018. PubMed PMID: 29043675.Grefen C and MR Blatt. Biotechniques 2012. PubMed PMID: 23066669.Hecker...

  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...pathway is regulated. You’ve read all the background papers you could get your hands on, formulated a hypothesis...immunoprecipitation-mass spectrometry strategies that isolate an antibody’s immune complexes and confirm interacting...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...available at Addgene. If you want to read more content related to CRISPR technology, subscribe to our CRISPR blog... plasmids Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

  4. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating with the previously established...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...

  5. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...string together all the words that I could remember related to this paper and/or biology in general until I... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...or talk extremely quickly. You realize, much too late, that you labeled your gene of interest incorrectly...

  6. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...’t just for DNA anymore! Cas enzymes have been isolated which target and cleave RNA through the same mechanism...and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less toxic ...mammalian cells than Cas13 (likely due to the lack of collateral cleavage). You can read about Cas7-11 in our ...

  7. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...“Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology by integrating...interventions you develop represent the whole human population. To learn a little more about where differences...clinical trials. And, as Janine Clayton points out, late-stage clinical studies that draw from a body of ...

  8. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...scientists are aware of the benefits of coaching relationships and do not know where to look for a coach. Many...thinking about what your goals are for the coaching relationship. Do you want to change jobs? Grow your professional...the coach to define how you will know that the relationship has been successful – what are the desired outcomes...

  9. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...cassette exchange. By utilizing recombinases to manipulate genomes, scientists can control expression of...and Tagging with Light (SPOTlight) as a way to isolate individual cells with unique spatiotemporal phenotypes...AAV-deliverable ABE.  Find the plasmid here Jesse Zalatan’s lab deposited plasmids for a chemically inducible...

  10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...found in type I CRISPR-Cas systems.   Four years later, the Sontheimer and Davidson labs discovered three... species of bacteria.  A note on anti-CRISPR nomenclature Acr family proteins are named for the type of...spatial, or conditional control of CRISPR activity Regulating Acr proteins by inducible promoters, light, or...

  11. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...F1 Once you’ve performed your first cross, congratulations! You’ve generated F1 mice and they are the ... strains throughout this process. In the F1 and later generations, mice that are homozygous wild type ...crossing over occurs between the two loci, F2 and later generations of mice can only be heterozygous for...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...maturation is even faster. Published just a few days later, Mohan Balasubramanian and colleagues showed the... then, when AAV-PHP.eB was injected three weeks later, that LY6A transgene rescued the enhanced brain ...Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism...

  13. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...The UCLA Library offers some Powerpoint based templates with different column numbers for a good starting...starting point. Adobe also offers free poster templates, although most are vertical.  Large central box...is relevant to the work you are presenting. The latter is catchy, but beware. A distracting background...

  14. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...measurement. Like a p24 ELISA, this method is relatively quick, providing results in less than a day, ...amplification of the original plasmid which can over-inflate results. A packing vector deficient in reverse ... serial dilutions of a viral prep, genomic DNA isolated, and primers targetting viral components or the...

  15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...protecting intellectual property, it worked away in isolation. It published a paper a year (or less) and limited...popped into our heads. Anything was fair game for speculation - in fact, our color changing flower project ... accomplish the color change until a few months later. RevBio accelerates with help from SynBio Axlr8r...

  16. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...managing a team. You’ve just been promoted, congratulations! You’re now a manager with your own team! But... other student stayed until he graduated. Years later I attended a formal training for new managers and...you motivated and help you grow in your role. The latter will make you feel discouraged and not valued which...

  17. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... other Cas effectors can’t, while maintaining relatively high efficiency. These alterations are achieved...Cutting both strands ensures that there isn’t a template strand left behind that the cell can use to repair...combination of chewing up one stand and cutting the template strand causes large deletions of up to hundreds...

  18. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...system. The paper also gives quantitative data for regulatory elements, which has not been available for plants...Nicotiana benthamiana. N. benthamiana is a tobacco relative and a good model for plant disease because it ...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...

  19. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...particular scientific subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426...and uncertainty in estimating parameters from relatively sparse data from China. An important additional...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...

  20. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...they want to understand the ultrastructural relationships that influence everything from cell division...physically separates molecules while maintaining their relative positions, allowing you to resolve individual ...accessible imaging equipment, ExM protocols utilize relatively inexpensive reagents and are readily adoptable...

  21. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ...Thanks to the Beer–Lambert law, the instrument can calculate a sample’s nucleic acid concentration based on... reading, distorting the final concentration calculation and potentially impacting downstream experiments...systems to better identify contaminants and to calculate corrected concentrations. However, such systems...

  22. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...mammalian cells can perform human-like post-translational modifications that bacterial and most yeast ...also need to know the extinction coefficient to calculate the concentration. At 280 nm, the extinction coefficient...protein standard and the concentration may be calculated using imaging software. Any bands on the gel ...

  23. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... If you are miserable at home or work, your relationships will suffer in both the short run and the long... learn to work within these bounds You can work later (in the evening, or in a few years) when life responsibilities...agree on before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The...

  24. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...singletons). I felt like anyone at a meeting who felt isolated at a conference for any reason was a Singleton...phenomenon in bacteria that occurs when bacterial cells regulate gene expression in response to changes in cell...structure with regard to the Mentor-Singleton relationship. Some of the variability was organic, as some...

  25. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...unlikely candidate. It was discovered as an antigen upregulated on activated lymphocytes and is commonly used...driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to a ~4.5 Mbp region...adeno-associated virus-PHP. B capsid fails to upregulate transduction efficiency in the marmoset brain...

  26. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...modifiers available. This system utilizes an RNA template containing the desired edit adjoined to the sgRNA...affect gene expression? dCas9 can be used as a platform to deliver various cargo to a specific DNA locus...single transcriptional activation domain - VP64.  Later generations of activators include the SunTag system...

  27. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...cells with VSV-G pseudotyped virus requires the upregulation of LDLR expression using other methods.  In ...Gutierrez-Guerrero, et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins...spumaviruses to human serum by galactosyl(alpha1-3)galactosylation. Journal of virology 71:6174–6178 . https:/...

  28. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...targeting the genomic site of interest. The donor template is supplied separately. To use this system, you... 37 °C.     The new pEcCas/pEcgRNA system We speculated that the gRNA on pCas that is specific to the... in the lack of recovered transformants. Our speculation motivated us to update the pCas/pTargetF system...

  29. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...which is the most commonly used bacteria in lab manipulations. Table 1 has a detailed list of the bacterial...leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, Salmonella spp....

  30. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum discussions...to see the questions first makes Reddit a great platform for those who aren’t so great at coming up with...whole point is to meet actual people to build relationships. I also like Twitter because I can get a little...
Showing: 511 - 540 of 792 results