...endonuclease domain, indicating that Cpf1 functions differently. Since Cpf1 loci are widely distributed across...could be adapted for genome editing. The use of a different nuclease could potentially overcome some of Cas9...Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in its low off-target editing rates, determined...shown the diversity of CRISPR-based systems. References 1. Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided...

.... While these differences may seem subtle they result in a key physiological difference between lenti-... system is that it involves transfecting four different plasmids into the producer cells (two packaging...as the viral long terminal repeats (LTRs) are different. As a result, lentiviral and retroviral packaging...

... to a conference. Really. No one will starve while you are away a few days. Also, your children will enjoy... yourself, “I’m not married,” or “I don’t have children, so my life will be under control.” Think again... hard not to do this. For a family with working parent(s), the sooner your kids can make a decent meal...

...0-5%. This blog post covers a brief review of different basic BE technologies and their adaptation for...Nickase Cas9 (D10A) Deaminase PAM requirements Reference CBE SpCas9 rAPOBEC1 NGG 6 SpCas9 Target-AID...Rice Research Institute, Cuttack, Odisha, India. Currently Kutubuddin is a Fulbright Visiting Scholar at ...improvement in the laboratory of Yinong Yang at PSU.   References 1. Komor, Alexis C., et al. "Programmable editing...

...that safe-targeting guides better control for the inherent effects, or background noise, of dsDNA breaks ...on-target activity. However, there was no significant difference in depletion of ricin sensitizer genes identified...Knockout Library are available from Addgene! References 1. Morgens, David W., et al. "Genome-scale measurement...libraries Find all of the CRISPR Pooled Libraries currently available from Addgene Other Resources Watch...

... Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient The first ...efficiency to Cre (Kranz et al. 2010). These differences in recombinase efficiency are important to keep...should be visualized at the same time from two different plasmids this approach becomes problematic as ...the genOway press release for more information. References 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M....

... The entry clone and destination vector carry different antibiotic resistance markers (indicated here ...STEP 1: Generate an Entry Clone There are a few different ways to generate our desired entry clone - human...interest: Find Gateway cloning plasmids here! References 1. Chee JY, Chin CF (2015) Gateway Cloning Technology... Digest Video Check Out Our Molecular Biology Reference Pages  ...

...of conversations with people who work in many different scientific fields. They also host AMA forums where...first makes Reddit a great platform for those who aren’t so great at coming up with answers off the cuff...future. The AMA was also cataloged for permanent reference by the Winnower. The only small downside to the...leaving out the term “implicit bias” which is apparently a troll trigger phrase these days). Redditors...

...immunogenicity and range of serotypes allowing preferential infection of certain tissues. However, packaging...equally potent Cas9 protein, the Zhang lab took a different approach. They analyzed over 600 Cas9 orthologs...Sontheimer lab used NmeCas9 with AAV to edit two different disease-causing loci in mouse liver.  Two other...have also been used to express base editors. References Chew WL, Tabebordbar M, Cheng JKW, Mali P, Wu...

...variations of Cas13 and Cas12a, are combined with different fluorescent reporters for each enzyme. In addition...Zetsche et al., 2015 ). Cas12a however recognizes a different PAM site and generates 5’ and 3’ staggered ends...Jennifer Tsang contributed to updating this article. References Abudayyeh OO, Gootenberg JS, Konermann S, Joung... Mondini A, Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L,...

...the lab can be applied anywhere! References and Resources References Grant, A. (2017). Originals how non-conformists...inclusivity. Work with and value people who think differently from you and you will arrive at unique solutions...ability to innovate. (Finkelstein, 2019) One issue currently plaguing the scientific community is supply chain...

... the Shapiro lab at Caltech.   References and Resources References Piraner, D. I. et al. Going Deeper...produce ultrasound contrast. We (the authors) are currently working on improving and optimizing ARGs, with...straightforward to engineer various cell lines from different tissues and mammalian species to produce gas vesicles...

...middle of proteins) and performs well in many different antibody-based applications.  IPI’s recombinant...virus 5 and their use in revealing antigenic differences between human, canine and simian isolates. J ...systems don’t always work across species due to differences in natural DNA repair pathways and spontaneous...at focal adhesions in living cells in several different contexts.   Figure 5: Left: Mechanism ...

...tracking experiments (Rizzo, 2009). With many different FPs that emit across the visible spectrum, researchers...of existing FP and epitope tags, researchers in Loren Looger’s lab have developed new molecular tags combining...antibodies. The “spaghetti monster” name is a reference to the deity of the social movement, the church... to the expanded use of smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance...

...testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR tests to diagnose COVID-...Cas12a, Cas13a does not require strict sequence preferences at the target site, while Cas12a requires a PAM...femto-aptomolar range. For more details on the differences between these methods, check out this table.  ...can follow her on Twitter @shra_krishna.       References Azhar, M., Phutela, R., Ansari, A. H., Sinha,...

... involved multiple people in tight locations. Conference rooms for on-screen presenters! Labs and benches...Zoom, everyone’s new favorite third-party video conferencing software. Interviewees could simply boot up ...while the camera is here, the operator is in a different room.   Design: The previous cycle made me...monitor and teleprompter being repurposed for a different shoot to create the How to Deposit Plasmids video...

...more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not so much...strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce a series of FP-RLuc8 fusions... #85201), Orange (OeNL; plasmid #85202) and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has...Promega cellular analysis products at Promega.     References 1. Hall, M.P., et al. (2012) Engineered luciferase...

...influence the evolution of their bacterial prey. Currently, receptor binding proteins (RBPs, the host recognition...thinking. Find her on Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage...phage therapy: new applications for old resources." Trends in microbiology 23.4 (2015): 185-191. PubMed PMID...bacteriophage-encoded glycan binding proteins and carbohydrates." Current opinion in structural biology 34 (2015): 69-77....

...with transcriptional activation. Created with BioRender.com. Tet1 demethylase Ronggui Hu’s lab has created... with increased transcription. Created with BioRender.com. Tet1 initiates cytosine demethylation of...with transcriptional repression. Created with BioRender.com. DNA Methyltransferase MQ1 Margaret Goodell's...Schwiesow contributed to updating this article. References Haldeman JM, Conway AE, Arlotto ME, et al (2018...

... strategies in C. elegans As brilliantly and irreverently illustrated in the parable of the Geneticist...the sequence added, leaving a 34 bp “scar”, but currently requires additional experimental manipulation....days. The only animals on a plate, other than the parental animal, are rescued progeny, which makes screening...more at his webpage or follow him on Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman...

...excitation light path. Each hole corresponds to a different location in the sample. As more than one hole ...one of four categories that are each suited to different types of modern day fluorescence microscopes (...means that the images will appear sharp without interference from blurry out-of-focus light. The most difficult...microscopy manufacturers have similar information References 1. Lichtman JW, Conchello JA. Fluorescence microscopy...

...over 100 entries spanning six different species and dozens of different expression sites. The Data...the AAV Data Hub is a beta project, and we are currently only accepting data for materials distributed ...

...search engines, which allow you to search many different antibody catalogs at the same time.  If you're...Library for Cancer Research Human Luekocyte Differentiation Antigen Workshops Immune cells Flow cytometry...others) Data repository From the Human Cell Differentiation Molecules group Addgene Data Hub Any ...

...has deposited If you’d like to write about a different topic, feel free let us know. What do you get ...audience of biological scientists from a variety of different fields. Exposure - With over 100,000 views each...interact with and discuss your work with many different people. Recent Guest Writers Have Included: Wenning...

...and the importance of realizing that different people have different management needs. How Crystal developed...and deciphering job listings. Mobility between different positions at Novartis. Chapter 7: How Crystal ...

... useful products under Professor Christopher E. French. Dr. Duedu was recently awarded plasmids from Addgene...7:12 - 7:11 : Kwabena's work in Christopher E. French's Lab at the University of Edinburgh - synthetic...bioresource to attract research funding. Using current resources to develop microbiology and molecular...

...that this service will be helpful to the many different scientific communities using plasmid tools. Details... our Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to ...other beta projects Read our Molecular Biology Reference ...

...demand. Q: Can you tell me a bit about your job currently? What is your day-to-day like? BN: I've made the...large companies, Genzyme and Biogen. They’re very different jobs. Being general counsel at a small start-up...companies. It's one of those "every day is something different" type of jobs, which really suits my personality...

...more than 30,000 neuroscientists over a 5 day conference? I feel like here at the annual Society for Neuroscience...late, late night socials. As a newbie to the SfN conference, I have been impressed by how the neuroscience...reports from bloggers at the meeting – my report is currently in the works. Or follow #SfN14 on Twitter for ...

...AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform for... to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition...