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  6. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ...

  7. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Microbes are everywhere and this book emphasizes the important roles microbes play in our ecosystem, examining...

  8. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...intersectional approaches is a growing field that we aim to support by growing our inventory of recombinases and recombinase-dependent...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...robust visualization tool to study the spatial and temporal arrangement of chromosomes and how these arrangements...

  15. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...Multiplex expression of gRNAs Four Base Editing Reporters to Monitor and Enrich Editing in Real Time Prime...

  23. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...point for compensation (Fig. 1B-C). However, it’s important to note that because you’re using beads and not...

  25. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...phenomena by generating an output which is directly proportionate to the amount of a given factor within a biological...

  26. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...Lenti particles enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear...

  27. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...agitation; a shorter sonication time could be an opportunity to decrease crosslinking. (Homogenization for...

  29. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...requestors for making their reagents available and for supporting scientific sharing. We’re very excited for more...

  31. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...

  35. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...they should be. Don't rush the process: It is important to give your plates enough time for any intact...

  36. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...find yourself doing your own conjugation, it is important to keep in mind that you will need to confirm ...

  38. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...extractions- if you need really clean RNA, it’s important to make sure that when extracting, to only take...

  39. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...

  40. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...subclasses, such as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary...
Showing: 681 - 720 of 780 results