We narrowed to 814 results for: Ran;

...library was developed when Carrie was a post-doc in Frank Stegmeier’s lab in Novartis Oncology.  Bhang did...lentiviral vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target...

...will allow you to set the amount of transcriptional and translational activity a cell has and control how...

...approved drugs so that your results are viable for translation to human patients. Picking receptors The receptor... specifically designed Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https...

...research or larger journals that publish about a broad range of topics.  Twitter paper bots: Twitter bots tweet...you expand your search. Results in your feed are ranked based on their influence on the network, or body...

.... The activity of SpRY was validated in double stranded cleavage and base editing applications, with high...solution? Almost! Assuming a PAM of NRN, 50% of randomly selected genomic loci will have a PAM located ...

...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which...clinical trial for SCD in 2022 as well. Hereditary transthyretin amyloidosis (hATTR) hATTR is caused by a variety...

... the Rare Genomics Institute to provide plasmid grants to researchers working on rare diseases through...the glycan biosynthetic pathway and have a wide range of clinical manifestations. PMM2-CDG is the most...

...fast-tracked development of diagnostics. Mostly transmitted by Aedes mosquitoes (aegypti and albopictus) ...and electronic companion technologies that has transformed the assay’s capabilities. Specifically this includes...

...tissue, placement above the site of stimulation guarantees that the majority of the opsins will receive ...tangled. Furthermore, to give the animal the full range of motion, a commutator allows the patch cable to...

...national issues. I applied and was successfully granted a travel award to attend the annual NPA meeting...to think about their career options, gaining transferable skills such as leadership skills can only assist...

...month - with only occasional supplementation with grant funds, money is always tight. Of course, unlike ...becomes obsolete amazingly fast, but with a wide range of skills among our members we’ve managed to learn...

...of an exogenous small molecule). Degron tags can range in size from several kDa up to ~35 kDa and are typically... because the system modulates protein post-translationally, unlike many other methods. Secondly, these...

...other words, newborn poop) visible through their translucent bodies. This is the ideal stage to select for... transgenic flies...

...’s group at the University of California, San Francisco, is approaching the Ras problem by exploiting ...lines. Further, for each plasmid in the kit, the transcript most commonly found across all TCGA tumor samples...

... end up on the N or C terminus of the transcribed and translated insert. If it is an empty vector with...

...for details). The following screenshots show the range of different terms we can use to find materials ...exact plasmid you need. The ability to apply a wide range of filters isn’t new to Addgene’s plasmid search...

...olds in a single evening. Grades K-5 cover a broad range of attention spans, skill levels, and STEM exposure...aspects of talking to 8th graders was the broad range of questions I received. I gave my presentation ...

...usually smallish peptides incorporated into a translated protein. As depicted in the accompanying cartoon...peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins...

...can use your cover letter to explain why your transferable skills make you a good candidate for the position...experience or skills, often by pointing to your transferable skills. In the above example, if you don’t have...

...where each base is recognized and recorded as a DNA strand is synthesized (Figure 1). Sanger sequencing requires...“nanopores” that are attached to the flow cell membrane and have an electrical current running through...

...of diagnosis) is about 35 years, but it usually ranges from 20-50 years and sometimes longer. There is...be Used to Study Cancer Drug Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional...

... general advice on how to meet people, how to transition out of academia, and other science career topics.... Although I didn’t start answering until 5pm, frankly, I was typing far before five. Fortunately I have...

...cell line contamination: Epithelial Mesenchymal Transition Is Required for Acquisition of Anoikis Resistance... to carry our independent quality control for a range of testing, from animal doping to forensics, DNA...

...heads and long legs that protrude from the cell membrane, physically connecting the extracellular environment...The actin cytoskeleton exerts tensile force, transmitted through the integrin, where it is resisted by...

...com­plex is very efficient at introducing double stranded breaks (DSBs) into genomic DNA in many cell types...homologous DNA repair template, such as a sin­gle-stranded oligodeoxynucleotide (ssODN), which contains the...

...will take to secure your first (R-level) research grant.  Set your timeline from there, then start identifying...techs’ financial books.  To find personnel salary ranges, go to online job information sites (like, indeed.com...

...Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR constitutive expression of...+), λ– EPI300   Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC...

...palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show ....2846 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R...

...project to validate some genomic changes, so this arrangement was ideal for everyone.  The cutting edge science...young scientists and PhD students to also explore a range of alternative careers outside of acidemia to have...

...should we use all tools as some kind of a meta/Frankenstein app? Or perhaps there is a clear winner, is ...enthusiasts, take a look at sgRNAcas9. Top tools for transferring CRISPR technology to a new organism: For obvious...

...mini marshmallows…). We’ll choose 4 submissions at random and deliver the prizes at the start of the New ...

...on the B. subtilis libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming efficiency...

...Hot Plasmid 3: CRISPR base editors with enhanced range and specificity. Listen to to the Segmennt of Improved...

...Gateway-Compatible Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen...

...where we can find the plasmid. While we cannot guarantee that everyone we reach out to will end up depositing...

...Noninvasive assessment of gut function using transcriptional recording sentinel cells! 🎵 On the 11th day...

...science support to our efficient and easy tech transfer process to our website and educational resources...

...hopefully make your life a little easier. These can range from technical tips as in our episode on Barcoding...

...where Addgene is located.  The export license, if granted, allows us to ship rabies plasmids and viral vectors...

...References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L., & Baker, D. (2003). Design...