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Showing: 681 - 720 of 860 results

  1. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...popular envelope and packaging plasmids as well as transfer plasmids. Download the orange lentivirus background...  You can find all of the plasmids that Addgene offers by visiting our collections page. Here, you can...

  3. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high throughput washing devices...your RMV to the landfill, it’s collected and transferred to the Red2Green processing facility, where it...

  5. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...professional and personal on Twitter. Or maybe scientist prefer to share links to research that isn’t behind paywalls.... And don’t forget to follow us @Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A ...

  6. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete or deplete... Jennifer Tsang...

  8. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...the moment you’re looking for it, or you may just prefer to troubleshoot yourself without contacting someone...our support team’s attention, where we can then offer the same high level of direct service you’ve come...

  10. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ... more than 75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression...

  11. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing...

  13. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...build reserves so the company is sustainable. The difference is what nonprofits do with their revenue. A nonprofit...One big reason is that scientists and technology transfer offices trust us to work for them with full transparency...

  15. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... brain region. While the method is powerful and offers immediate measurements in a live animal that can...other biosensors in our AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo brain microdialysis...

  16. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ... the biomedical research field! References and Resources References Fellmann, C., et al., Cornerstones...

  18. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...Optogenetics Ofer Yizhar New viral prep pAAV_hSyn-DIO-PdCO-mScarlet-WPRE AAV1, AAV5 Optogenetics Ofer Yizhar...

  19. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...and the insert size should be a max of 500 bp (preferably). I compiled a list of three options from the...clones on an electrophoresis gel in order to differentiate which clone had successfully incorporated Syntaxin3...

  20. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...community, but also allows cross-interrogation of different datasets that might otherwise be hard to identify...breast cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot updated 2024...

  22. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...provides talks and career support for a range of different scientific disciplines, as well as social and ...members as well as arranging collaborations and conferences for their students. Another large goal of the...

  24. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... to this list. Need feedback on their work but prefer to be approached as peers rather than subordinates... series as it is published. Good introductory reference books on managing people: Skills for New Managers...

  25. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...project establishes your professional brand. It offers colleagues insight into your interests and will...will I take and what can I learn, learning the difference between good and bad data, and knowing how to...

  26. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect...

  28. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His...

  29. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...but when it came time to add author details a different person in the lab wanted to be the first author...scientists in SciComm careers Check out our series on transferable skills ...

  30. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...

  31. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...rumors spread in our building that someone from a different company had tested positive for COVID-19 (it turned...studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority...

  32. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...whether any given antibody is available through different companies (Bandrowski et al., 2016). It’s also... the antibody sharing landscape as well.     References Andrews NP, Boeckman JX, Manning CF, Nguyen JT...

  34. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...actually from a completely unexpected finding in a different experiment, this tool was developed because a ...doing and sharing science in the first place?   References and Resources More resources on the Addgene blog...

  35. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...restriction enzymes that can both function in the same buffer, as this will save time later In our example,...Resources on Addgene.org Check out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the...

  36. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...translocations. It also provides researchers with many different ways to control gene expression. Get the full ...Joanne Kamens explains what career coaches do and offers up some career coach suggestions. 8. A Match Made...

  37. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...light-induced cell ablation with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and...using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato, M. A., Onuoha...

  38. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...

  39. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ... investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An... of the scientific research infrastructure. References Chu H, Chan JF-W, Yuen TT-T, Shuai H, Yuan S,...

  40. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...time for exercise. Many studios and gyms are now offering online classes and participating in these is a... Jennifer Tsang...
Showing: 681 - 720 of 860 results