960 results

Addgene plasmid pages now show whether a plasmid has been cited in other articles. Use the "References" link to view the articles citing your plasmid.

June 16, 2015, 2:15 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

June 2, 2015, 6:06 p.m.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.

May 22, 2015, 4:58 p.m.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

May 12, 2015, 1:32 p.m.

Tips for writing your scientific manuscript, including how to search for and organize papers.

May 5, 2015, 3:54 p.m.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

April 24, 2015, 2:39 p.m.

Addgene's interviews Chris Voigt of MIT about the intersection of CRISPR and synthetic biology and his new work building circuits with CRISPRs.

April 22, 2015, 2:06 p.m.

Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

April 16, 2015, 3:45 p.m.

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

April 9, 2015, 3:29 p.m.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

April 7, 2015, 4:21 p.m.

As part of Addgene's Management for Scientists, this post describes the differences between managing vs. leading and the skills associated with each.

March 31, 2015, 4:08 p.m.

Learn about how Addgene has shipped over 500,000 plasmids and where those plasmids go.

March 26, 2015, 3:24 p.m.

Learn more about the Developmental Studies Hybridoma Bank (DSHB), a repository for monoclonal antibodies, that distributes antibodies and their associated hybridoma cell lines.

March 10, 2015, 2:25 p.m.

Read about artists who are turning science into art by using scientific techniques to view the world from another vantage point.

March 2, 2015, 3:13 p.m.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

Feb. 24, 2015, 7:50 p.m.

Learn how effective delegation allows you to make the best use of you and your team's time and skills in this Management for Scientists post.

Feb. 20, 2015, 8:30 p.m.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Feb. 18, 2015, 3:09 p.m.

Learn more about E. coli strains used for protein overexpression.

Feb. 10, 2015, 3:06 p.m.

Focusing on the importance of giving feedback to your employees, this is the third installment of a 5 part series on Managing People for Scientists.

Feb. 6, 2015, 2:46 p.m.

Addgene data on CRISPR and synthetic biology plasmids deposited to the repository and distributed around the world.

Feb. 4, 2015, 3:58 p.m.

What is a career coach and how can scientists find the right one for them?

Jan. 29, 2015, 1:25 p.m.

Dora Farkas, career coach for scientists, discusses how to identify difficult supervisors and outlines 7 strategies to resolve conflicts.

Jan. 21, 2015, 1:30 p.m.

Overview of the 2015 Keystone Symposium: Precision Genome Engineering and Synthetic Biology from Addgene's own Eric Perkins, including the state of CRISPR.

Jan. 15, 2015, 1:50 p.m.

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Jan. 13, 2015, 3:47 p.m.

Addgene's Executive Director, Joanne Kamens, shares advice for management of scientists on how to seek feedback from your employees.

Jan. 8, 2015, 2:34 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

Tips for preparing and writing a resume when transitioning from an acamedic to a non-academic science career.

Dec. 18, 2014, 4:06 p.m.

Find out which of Addgene's blog posts were most popular in 2014. Also, learn how to become a guest blogger for Addgene.

Dec. 16, 2014, 8:01 p.m.

Protein tags can be used for purification, detection, localization, and more. Learn more about the molecular tool box of tags and fusion proteins.

Dec. 11, 2014, 4:26 p.m.

Learn what makes a good manager, what factors led to job satisfaction, and how specific strategies for managing scientists.

Dec. 9, 2014, 2:22 p.m.

Discussion of how the notions of scientific impact are evolving and suggestions for tools you can use to evaluate your impact.

Dec. 5, 2014, 2:54 p.m.

Addgene interviews Hodaka Fujii of Osaka University to learn about his lab's enCHIP system, their new CRISPR tools, community involvement, and more.

Dec. 2, 2014, 7:23 p.m.

Exercised or endurance-induced expression of PGC-1a1 in skeletal muscle protects the brain from depression in mice.

Nov. 25, 2014, 4:02 p.m.

Update from the 2014 annual meeting of the Society for Neuroscience (SFN14) in Washington, D.C. as seen from the Addgene booth.

Nov. 18, 2014, 7:15 p.m.

An all-in-one CRISPR/Cas9 Multiplex Genome Engineering System for transfecting and editing cell lines.

Nov. 12, 2014, 3:54 p.m.

Learn more about the common E. coli strains used in the lab.

Nov. 7, 2014, 2:56 p.m.

The basics of Fluorescence Resonance Energy Transfer (FRET), including tips and parameters to consider when using FRET for your experiments.

Nov. 5, 2014, 3:41 p.m.

Halloween is a favorite holiday at Addgene. As we look forward to this year's Halloween festivities, check out past costumes from our Addgenies.

Oct. 29, 2014, 5:26 p.m.

Five steps to executing a great panel discussion - from planning to moderator control to audience participation.

Oct. 22, 2014, 1:57 p.m.

The basics of Ebola virus and links to helpful resources for scientists interested in studying Ebola.

Oct. 16, 2014, 3:01 p.m.

Tips on how to choose compatible fluorescent proteins for multi-color imaging.

Oct. 9, 2014, 3 p.m.

Summary and thoughts from the post-doc driven 2014 Future of Research Conference in Boston.

Oct. 7, 2014, 3 p.m.

Interested in sharing your lab protocols with others? Here are 7 software apps that can help you share and edit your protocols with other scientists.

Sept. 23, 2014, 3:05 p.m.

Emmanuelle Charpentier and Jennifer Doudna awarded for their CRISPR/Cas9 research at the 2014 Johnson & Johnson's Dr. Paul Janssen Award dinner.

Sept. 15, 2014, 6:05 p.m.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Sept. 9, 2014, 8:20 p.m.

Learn how postdocs are organizing consulting groups and symposia, such as the Future of Science, as a way to take charge of their career training.

Sept. 2, 2014, 3:05 p.m.

Learn more about how to verify your plasmid via a diagnostic restriction digest.

Aug. 28, 2014, 3:34 p.m.

Read advice for grad students and postdocs applying for science careers in sales and marketing, including tailoring your CV.

Aug. 21, 2014, 3:15 p.m.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Aug. 19, 2014, 3:39 p.m.