We narrowed to 568 results for: CHI
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TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....rural health clinic in Ghana. My role involved teaching the nursing students and community health workers... world. The research project was hard; I was researching the epidemiology of breast cancer and I loved...
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Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....for mammalian expression and AAV plasmid sets launching later this year! Find Addgene's own Lentiviral...Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....alternative sequencing approaches have been developed. Cas ChIP-Seq, where only DNA sites bound by Cas are sequenced...for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding sites within... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....Although there are different ways to ultimately achieve success, we find it is usually faster and easier...large number of different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....need! How does it work? There are several ways to achieve multiplex IF. One of the most common approaches...the sample, either by chemical treatment or photobleaching or by stripping out the antibodies, then start... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m.... particularly interested in, but I’d recommend watching the whole livestream for a more holistic understand...future work cost both time and money with estimates reaching in the billions of dollars (Freedman et al 2015... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog PostPublishedDec. 15, 2016, 3:57 p.m....PMCID: PMC307639. 2. Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald...recombination system for chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m.... target. There are a multitude of options for achieving success in a science policy career with many opportunities...organizations, working in student government, and teaching and mentoring others (see more in Figure 2). Starting... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m.... mammalian cells. Type IV Type IV is the middle child (like me!) of CRISPR types — weird and poorly understood...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence...a slide, or whole organs. Because the tissue architecture is preserved, this technique allows you to not... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m....Cofsky et al., 2022; Osuka et al., 2018). Each matching DNA:RNA pair gives just enough stability for the...Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m.... inhibitor with a degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are ...plasmids from this paper at Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for ... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m.... can also download the software for use on any machine with OpenCL enabled hardware. This OpenCL dependency...genomes), then it is worthwhile buying a dedicated machine. On the offline front, there is a Python script... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....versatile, but it requires careful optimization to achieve the best efficiency. The type of edit, genomic ...Liu lab contributed an improved prime editing “architecture” that can be combined with any of the PE2-PE5... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....With both siRNA and shRNA, it can be difficult to achieve sufficient knockdown to enable robust assessment...S., Wright, A. K. A., Sousa, J., Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P. (2019). Validation... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....these common antibody applications. Thanks for watching, and we hope you found the video radd-ically helpful... -
Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog PostPublishedSept. 7, 2021, 1:15 p.m....the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the Lab Bench... -
The 12 Days of CRISPR: 2021
TypeBlog PostPublishedDec. 28, 2021, 2:15 p.m....prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog PostPublishedDec. 5, 2023, 2:15 p.m....DuanVisualizing Your Plasmid Request Data Author: Joachim Goedhart Created in collaboration with Addgene...