Skip to main content
Addgene

We narrowed to 568 results for: CHI

Showing: 341 - 360 of 568 results
  1. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...rural health clinic in Ghana. My role involved teaching the nursing students and community health workers... world. The research project was hard; I was researching the epidemiology of breast cancer and I loved...
  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...for mammalian expression and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral...Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed...
  3. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...alternative sequencing approaches have been developed. Cas ChIP-Seq, where only DNA sites bound by Cas are sequenced...for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding sites within...
  4. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Although there are different ways to ultimately achieve success, we find it is usually faster and easier...large number of different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression...
  5. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...need! How does it work? There are several ways to achieve multiplex IF. One of the most common approaches...the sample, either by chemical treatment or photobleaching or by stripping out the antibodies, then start...
  6. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ... target. There are a multitude of options for achieving success in a science policy career with many opportunities...organizations, working in student government, and teaching and mentoring others (see more in Figure 2). Starting...
  7. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... mammalian cells. Type IV Type IV is the middle child (like me!) of CRISPR types — weird and poorly understood...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various...
  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence...a slide, or whole organs. Because the tissue architecture is preserved, this technique allows you to not...
  9. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...Cofsky et al., 2022; Osuka et al., 2018). Each matching DNA:RNA pair gives just enough stability for the...Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization...
  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... inhibitor with a degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are ...plasmids from this paper at Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for ...
  11. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...versatile, but it requires careful optimization to achieve the best efficiency. The type of edit, genomic ...Liu lab contributed an improved prime editing “architecture” that can be combined with any of the PE2-PE5...
  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...With both siRNA and shRNA, it can be difficult to achieve sufficient knockdown to enable robust assessment...S., Wright, A. K. A., Sousa, J., Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P. (2019). Validation...
  13. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...these common antibody applications.  Thanks for watching, and we hope you found the video radd-ically helpful...
  14. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me...
Showing: 341 - 360 of 568 results