We narrowed to 360 results for: CRISPR
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TypeBlog PostPublishedOct. 27, 2015, 3 p.m.... genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over...
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Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate as they... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m....Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources on Addgene.org... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m.... Mice can be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m....revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous... -
Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m.... interactions Learn how biotin can be used with CRISPR Additional resources ThermoFisher Scientific... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m.... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519... Gilbert Labs have previously deposited their CRISPRoff system — a programmable epigenetic editor consisting...deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins than SpyCas9... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog PostPublishedDec. 15, 2016, 3:57 p.m....Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering Resources... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m.... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....sequences cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....expressed at endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly used to produce KO... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m.... 96 parts including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor...promoters, and inhibited gene expression using CRISPRi. The kit functions in the model cyanobacterium ... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog PostPublishedMay 18, 2017, 2:30 p.m....Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org...