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  1. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...listeners to be thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles...term, but are worried that this might sound condescending, consider explaining the term on a slide but...

  2. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin...Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control of neural...

  3. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...specific substrate. The emitted light, or chemiluminescence, is detected on x-ray film or with specific..., Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender AR (2019) Antibody validation for Western blot...

  4. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...identify possible gene function. In this post, I will describe a useful and cost-effective method that our lab...and probe with live cells The following is a description of a technique published by Simpson, Sacher, ...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...in practice integration can occur. Okita et al describes a protocol for generating iPSCs by plasmid transfection...transduction, such as the polyarginine peptide described in Zhou et al and Kim et al. This is considered...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...without introducing DSBs at all. Base editing to the rescue! Base editors chemically modify DNA bases without...named ABEmax. In 2020, two papers were published describing additional ABEs evolved from ABE7.10 that have...

  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... as well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements... Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids. Selected...

  11. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ... de nuestra colección de libros electrónicos. Descargar CRISPR 101: Una herramienta de edición Addgene...

  13. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ... Team at the Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford...

  15. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...institution. Requesters will need to provide a brief description of the applicable research project when purchasing...

  16. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ... of the secretome, ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more (find the...

  17. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating Choices Paul...

  18. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...We have Plasmid Kits for microbial work with fluorescent proteins, synthetic biology, gene cloning and...

  20. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells   ...and many more...
Showing: 341 - 360 of 546 results