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We narrowed to 553 results for: ESC

Showing: 341 - 360 of 553 results

  1. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...plasma membrane. In previous publications, they described tools to control formation of lamellipodia and... unnatural head groups for applications like fluorescent labeling via click chemistry.    Figure 4: SuperPLD...

  2. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...feedback. Many of these documents are too vaguely described in other sources of guidance or the document examples...in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell imaging approaches. ...

  3. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ..., and ultraviolet light. In this post, we will describe the mechanism of homology directed repair, one...generally preferred for large insertions such as fluorescent proteins or selection cassettes. This class of...

  4. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...listeners to be thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles...term, but are worried that this might sound condescending, consider explaining the term on a slide but...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin...Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control of neural...

  6. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...specific substrate. The emitted light, or chemiluminescence, is detected on x-ray film or with specific..., Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender AR (2019) Antibody validation for Western blot...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...identify possible gene function. In this post, I will describe a useful and cost-effective method that our lab...and probe with live cells The following is a description of a technique published by Simpson, Sacher, ...

  11. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...in practice integration can occur. Okita et al describes a protocol for generating iPSCs by plasmid transfection...transduction, such as the polyarginine peptide described in Zhou et al and Kim et al. This is considered...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong et al. (2013) Science ...co-transfect in HR donor. Alternatively, a new system described recently is the double nickase system, with PX335...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...without introducing DSBs at all. Base editing to the rescue! Base editors chemically modify DNA bases without...named ABEmax. In 2020, two papers were published describing additional ABEs evolved from ABE7.10 that have...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... as well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements... Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids. Selected...

  15. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ... de nuestra colección de libros electrónicos. Descargar CRISPR 101: Una herramienta de edición Addgene...

  17. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ... Team at the Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford...

  19. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...institution. Requesters will need to provide a brief description of the applicable research project when purchasing...

  20. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating Choices Paul...
Showing: 341 - 360 of 553 results