We narrowed to 685 results for: Eng;
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TypeBlog PostPublishedMarch 16, 2017, 1:06 p.m....twitter @aigilson. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional...
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Introducing Addgene's Microbiology Pages
TypeBlog PostPublishedJune 13, 2016, 7:08 p.m....Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ... -
5 Science Rap Videos & Their Creator: Tom McFadden
TypeBlog PostPublishedFeb. 9, 2017, 3:30 p.m....sciencewithtom.com. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional... -
An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success
TypeBlog PostPublishedDec. 8, 2015, 3:30 p.m....discoveries from her lab. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. References... -
New Podcast Segment: Hot Plasmids
TypeBlog PostPublishedMay 17, 2017, 3:37 p.m....Yeast Prototrophy Kit Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional... -
Grad School Advice Part 1: Picking a Lab and a Project
TypeBlog PostPublishedSept. 1, 2016, 2:30 p.m....Vincent's tweets. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional... -
Grad School Advice Part 2: Building Community
TypeBlog PostPublishedOct. 6, 2016, 2:30 p.m....Vincent's tweets. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....You can try a few different lysing buffers and strengths. If that’s not working, consider using a subcellular...incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect... Different lysing buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....details. "Understanding this structure may help us engineer around the current limitations of the Cas9 complex...said study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future,... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex. The Covert...which fluorophores can be multiplexed together. Challenging to Design: FRET reporter activity depends on... -
Your Top Requested Plasmid in 2016!
TypeBlog PostPublishedJan. 11, 2017, 8:47 p.m....2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....missing out on an opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an ... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....for mammalian gene silencing. TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger...labs around the world. Thank you! It's been a challenging and rewarding journey since starting Addgene.... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming...tools in your experiments. Many thanks to Hongkui Zeng’s lab for providing this AAV vector! Additional ... -
Scientist Networking: What is an Informational Interview?
TypeBlog PostPublishedJuly 1, 2014, 3:42 p.m.... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....twitter @aigilson. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m.... However these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania. References... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog PostPublishedDec. 17, 2013, 2:47 p.m....Navigating a fulfilling career in science can be challenging – is advice and guidance from only 2 people enough...doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools...blog post. References and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou...