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We narrowed to 450 results for: HA tag

Showing: 341 - 360 of 450 results

  1. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...yeast that same day.  The field of synthetic biology has led to advances in biofuel generation, green chemistry...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

  2. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...the indicated function. One huge reason CRISPR has become such a popular genome editing tool is its developers...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer ...that particular organism available at Addgene. Percentages represent the fraction of that total with the...

  3. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...and the master plasmid in one step. The kit also has other unique aspects that make it especially well-suited...for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be more efficient...together, there are occasional errors. That can be advantageous if you are trying to knock out a gene. One can...

  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...Cytotoxicity can also be cell type dependent: if a cell has few receptors for a specific envelope protein, it...lentiviral vector to be transported retrograde and also has higher neuronal transduction efficiency and a better...different viral membranes, tropism, and advantages and disadvantages: Table 1 (Joglekar et al., 2017)  Table...its own, it needs to be pseudotyped. This takes advantage of the EnvA-TVA receptor-envelope system (Wickersham...

  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...mammalian cells. Each of these systems has their advantages and disadvantages. Briefly, bacterial and yeast cells...recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids...

  6. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...purify DNA, but rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the ...chaotropic salt sodium iodine (NaI) solution. NaI has two purposes in this protocol: 1) it solubilizes ...DNA without a kit. DNA purification kits have advantages: they are convenient and provide uniform, consistent...least entraps nucleic acids. The Botella lab took advantage of this property and developed a protocol that...

  7. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...cornerstone for The Addictive Brain. Our community has also requested live question and answer sessions ...sessions led by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming...participate in our one-hour Live Q&A session on Instagram every Sunday. Undergraduate and graduate students...background. Social media is a powerful tool. Use hashtags effectively. This would help you direct your content...

  8. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ... and microbe cookies! 🤗 🤗 🤗 We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷...showcase it all!           View this post on Instagram                       A post shared...dyed with real microbial pigment. Check out the hashtag to see the description of all the ornaments. #DeckTheLab...

  9. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology and subsequently obtained...Newland is General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics...this past year and a half. My wife and I went to Patagonia in February of 2020 and came back just a week ...

  10. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...editor in 2016 (Komor et al., 2016) and since then has been trying to expand their precision editing capabilities...contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable.... A phage encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate ...

  11. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine...been approved by the FDA, and this mode of delivery has a lot of clinical promise moving forward. What viruses...acid-based vaccines. These vectors provide several advantages over traditional vaccines, including 1) cellular...

  12. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...in the messaging stream. Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect...easy favor for people in your network. If someone has connected with me, I consider them fair game for ...many academic scientists are starting to see the advantages of using social networking to start, build, and...

  14. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...   In conclusion, the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal...editing systems in bacteria. Our pCas/pTargetF system has been one of the most popular genomic editing systems...the newly generated pEcCas/pEcgRNA system.   Advantages of the pEcCas/pEcgRNA system Compared with the...

  15. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...target is known to be absent naturally or in which it has been knocked-out/knocked-down. “No primary”, pre-... reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...fluorophores to visualize multiple targets at once. An advantage of multiplexing is that you can ask how multiple...choose can make or break your experiment, so take advantage of all the available resources and give yourself...

  16. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...served by a one-sample test as the standard value has no variation that needs to be accounted for. ANOVA...multiple range test, and several others. Each test has different likelihoods of returning false positives...to a single group. Dunnett’s is more powerful and has a lower error rate than the multiple comparison tests...dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA and MANCOVA...

  17. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...would not end up in a life sciences career but life has a way of making unexpected decisions. As someone ... I was able to express my visions for what my Instagram posts would look like. While working remotely ...continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed fellow Addgenies about their role... Some of recent Meet an Addgenie posts on Instagram! From this project, I observed what it was ...

  18. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Minamimoto’s lab known as deschloroclozapine (DCZ) has been developed to meet these requirements. TL;DR..., both techniques offer researchers different advantages and limitations.  The four main factors in which...allow for light delivery. Chemogenetics poses the advantage of being less invasive and more flexible as it...controlled EndogenousNot easily controlled Unique advantages Mapping the brainDissecting circuits Studying...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...might be more successful. “Shock and kill” has the advantage of killing cells infected with HIV-1 to deplete...tough job! Kamel Khalili’s lab at Temple University has demonstrated two potential strategies for CRISPR-... treatment with CRISPR/Cas9 SAM increased the percentage of cells with activated HIV-1, measured via HIV...

  20. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...characterization of over 1,000 mutations, and the group has deposited several hundred plasmids from this study...lentiviral constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (... any mutations that that confer proliferation advantages will be revealed in the absence of growth factor...
Showing: 341 - 360 of 450 results