We narrowed to 388 results for: HAL;

1. Hot Plasmids: Fall 2024

   Type

   Blog Post

   Published

   Nov. 21, 2024, 1:30 p.m.

   ... Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, A. R. (2024...

2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...essential genes whose knockout would cause cell lethality. The Krüppel associated box (KRAB) is a naturally...

3. New CRISPR Tools: Cas7-11 and PASTE

   Type

   Blog Post

   Published

   Feb. 17, 2022, 2 p.m.

   ...right integrase for this new approach proved a challenge. The old standby of CRE/flp didn’t work – it turns...

4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang...

5. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through how...

6. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   Published

   Sept. 17, 2019, 12:40 p.m.

   ...stay tuned!  References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic...

7. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...severe health conditions such as nonsyndromic holoprosencephaly where the brain fails to divide properly into...

8. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...Induced Pluripotent Stem Cells (iPSCs): Benefits, Challenges and the Road Ahead." Journal of Alzheimer's disease...

9. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   Published

   Nov. 14, 2017, 2 p.m.

   ...development (or lack thereof) One of the biggest challenges facing students today is that many graduate and...

10. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ... less time consuming and/or less technically challenging. Alongside there is a second-line team that takes...

11. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...depending on their serotype, are particularly challenging. Their efficacy in any given system can be affected...

12. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...was recognized by meeting organizers in their town hall.  Iterative improvement - Making Binning Singletons...

13. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...technology has been studied and used in assays for over half a century; the Addgene blog has an excellent primer...

14. How to Write a Scientific Review Article

    Type

    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...

15. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers...

16. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...what they are doing, however, may face greater challenges. Current salary information that we have been...

17. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...

18. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ...

19. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    Published

    June 28, 2016, 10:59 a.m.

    ...stranded break at the target site, allowing the two halves of EGFP to recombine by homology directed repair...

20. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...than ICSS. The test chamber is divided into two halves that are distinguished visually through different...