We narrowed to 771 results for: Ina;
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TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....American system), in 2009 (at the beginning of the financial recession) I knew obtaining work in scientific...facility, where I tested materials for bacterial contamination. It was a really interesting job at first, everything... are rapidly expanding, as this might not be sustainable. After working at this company for several years...would produce the best outcome for a specific combination of genetic mutations. This is a particular challenge...
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Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for...receptor binding protein hit and can go back to the original plate to identify which phage gene encodes that... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...colony expressing a functional binding protein was originally located. Now you can grow up your colonies of... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....engineered proteins responsive to light in combination with retinal) variations designed to give researchers... R., Gunaydin, L. A., Hyun, M., Fenno, L. E., Gradinaru, V., Yizhar, O., & Deisseroth, K. (2012). Principles... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....testing several vectors and packaging cell line combinations, Miller et al. showed that infectious wild-type...are capable of transducing certain quiescent or terminally differentiated cells such as macrophages and ...research due to the possibility that mutation or recombination with human retroviruses could lead to RCL. ...of retrovirus packaging cell lines to avoid recombination leading to helper virus production." Molecular... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....includes: Michelle, the Senior Scientist who coordinates all kit creation and quality control efforts ...once famously said “I think this is the most extraordinary collection of talent, of human knowledge, that...successfully doled out to multiple people: someone to coordinate, someone to decide, someone to delegate, etc....Next Great Discovery is exactly that: a myth. The original Dr. Frankenstein from Mary Shelley’s novel worked... -
Help Us Name Blugene’s New Virus Friend
TypeBlog PostPublishedJan. 7, 2020, 1:55 p.m.... contest is now closed. Stay tuned to see the finalists and vote for your favorite! Our mascot who has...neutral name. The Addgenies will choose up to four finalists and we’ll ask you to vote for your favorite name...We hope you submit your best ideas because the finalists will receive some newly designed Addgene swag.... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....structures are more stable and local movements predominate (e.g. a basal progenitor cell migrating to the...fluorescent proteins (Salomonnson et al. 2012). Finally, it is worthwhile to test the photostability of...used to study B and T cell dynamics within the germinal center of murine lymph nodes. However, one must...marking and tracking cells is the the Cre/lox recombination system. A researcher can flank fluorescent proteins... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m....Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan The job of scientists to observe how things... and generation is in the veracity of its work. Alina Chan is a postdoctoral researcher at Harvard Medical...saying that climate change is a hoax. This has culminated in the current President holding and campaigning...interactions, and science education. Photo cred Alina Chan. Seattle, Washington, USA 47.6062°N 122.3321... -
Find and Share AAV Data with Addgene's New AAV Data Hub
TypeBlog PostPublishedOct. 8, 2019, 12:33 p.m....and important details that you won’t find in the final publication and we have already collected over 100...accelerate your research. Sincerely, Melina & The Addgene Team ... Melina Fan... -
An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success
TypeBlog PostPublishedDec. 8, 2015, 3:30 p.m....Working on the Eye 9:05-10:16 : Cell Targeting in Retinal Gene Therapies 10:17-14:34 : Progress in Treating...type-specific manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-1341. PubMed... retina... -
Bringing Renewable Energy to Addgene
TypeBlog PostPublishedAug. 27, 2019, 1:08 p.m....renewable electricity is certainly not the only sustainability effort we’ve implemented at Addgene. The kitchen...check out Leila Haery’s blog series, “Bringing Sustainable Practices to the Lab.” Whether participating...blog Subscribe to the Addgene blog Read our sustainability in the lab blog posts Read another blog post... -
New Podcast Segment: Hot Plasmids
TypeBlog PostPublishedMay 17, 2017, 3:37 p.m....of His-tagged human kinases in bactera. Listen to Our Segment on The Human Kinase Domain Constructs Kit...Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's Lab -... -
Summer Fun at Addgene!
TypeBlog PostPublishedMay 12, 2022, 1:15 p.m....The weather is finally warming up in Boston and that means summer is right around the corner. Here at...other video series. And if you haven’t seen our webinar “Navigating an Expanding Landscape for Engineered...which we will be posting in the next few weeks.) Finally, Addgenies love sunshine and warm weather - check... -
Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up
TypeBlog PostPublishedApril 5, 2016, 2:30 p.m....taking steps to increase plasmid distribution and disseminate knowledge around the globe. Addgene’s progress...inception in 2004. In the near future, we hope to eliminate much of white space on this map and increase plasmid...the Addgene Blog Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....project that would be a big deal if it worked out.”- Melina Fan, Chief Scientific Officer “Choose your PhD ... Tsang, Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting...customized letter to the place that you are applying.” - Melina Fan, Chief Scientific Officer If there’s a career... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....viral vector is used to deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It... work with in neurons or you might use it in combination with Flp-FRT. Additionally, you could use the... cre-recombinase... -
Celebrating Accomplishments in the Lab
TypeBlog PostPublishedMay 8, 2014, 3:07 p.m....lab heads have revealed that a combination of hard work, determination, passion, and patience are required... time for fun and life outside of the lab? This final post in the PI Interview Series investigates how... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m....distributor partners, with the launch of antibodies in China and expansion of viral services available in South...Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed at IPI. The first...partially through work with our distributors in China, Korea, Japan, India, and Brazil. Through an expansion... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....North Carolina Research Triangle area, scientists give talks and science cafés at the North Carolina Museum... surprised to find that his friends and family finally understood why he cared to study mice on some distant... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m.... precious in less resourced areas of the world. Nina Dudnik, Founder of Seeding Labs, tells the story...Outreach Scientist, Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol for making competent...this was hilarious. Birth of the baby gel box Finally, taking conservation to new levels, I was bothered...