We narrowed to 823 results for: LARS

...reproducibility of experiments. Inbred mice are particularly useful for understanding a specific effect related...that alters or eliminates the expression of a particular gene. Targeting vectors used to create knockout... a way to track successfully engineered cells. Similar to transgenic mice, knockout mice are engineered...off-target effects on regions of the genome with similar homology to the site of interest or create mosaic...recombination of floxed sites in the cell can occur. Similar systems include tetracycline or doxycycline mediated...

...perform more modern techniques used in genetics or molecular biology because the experiments are either not...templates which they can easily generate off of a circular plasmid via PCR. Typically, to make these constructs...complementary DNA (cDNA), and then use PCR and molecular cloning to insert the cDNA into the desired plasmid...those who are interested in performing their own molecular cloning and investigating genes not in this collection...vector backbone. The empty vector PLL4, which is similar to PR-T4P, is available from Addgene. If you use...

...the bait at a particular point in time, i.e. when the bait is interacting with a particular protein partner...specifically identify proteins that interact with a particular protein  complex as opposed to an individual ...partner or when the bait is part of a particular protein complex. Not performed in a native cell context...bait proteins stop interacting. This feature is particularly useful for in-depth characterization of dynamic...

... the digital music revolution hit. I predict a similar revolution for antibodies. Recombinant antibody...that may not work well in animals because of similarities to host proteins. After the antibody of interest...long-term stability, consistency between batches, and molecular definition of recombinant antibodies are essential...US alone (Bradbury & Plückthun, 2015a). With molecularly defined antibodies that do not change over time..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are...

...friendly, to own the process entirely remains a large undertaking. Priority number one was to ensure that... There are regions of some plasmids that are particularly difficult to sequence and assemble, including...regions that are GC rich. Since NGS assembles a larger sequence from many smaller sequences, regions with...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are...Viral preps and pooled libraries In addition to regular plasmid sequencing, many of you will be familiar...

... are your best bet to figure out how to use a particular reagent.  We also have links to later papers ...look! This stuff works!” These papers can be a particularly good resource if you are considering using a...serious science questions, consider using Google Scholar, which searches scientific articles, patents, and.... Addgene keeps a Document of Gratitude from particularly enthusiastic customers who have enjoyed their...combatitive. Not every question is answerable, particularly when it comes to technical questions. BUT, I...

...CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming...Szostak’s lab continues its work on the origin of cellular life. His lab has long hypothesized that life ...Nikolaus Rajewsky from the Max Delbruck Center for Molecular Medicine presented another unexpected finding ...regulatory RNA field. The Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These...abundant in neuronal synapses. shRNA silencing of one circular RNA they have identified resulted in a cell migration...

...Management skills”. The NPA states that Postdoctoral scholars should have the skills and techniques needed to... how to adapt to different situations and, in particular, to different styles of leadership. As the Chair...Committee would change format depending on the particular style and requirements of the new Board Members... more independently and some others would need regular re-direction and alignment with the overall mission...organization to develop these leadership skills in particular because every year it organized a “Committee ...

...after receiving her degree she was faced with a similar conundrum – she had the skills, training, and motivation...our ideas got pretty good. We started doing extracurricular research on some of them to see how feasible...in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and...company, and that’s what we want it to do. It’s exhilarating to have that sort of freedom. It can also be...and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois ...

...pathways and represent the largest family of human drug targets. To enable large-scale profiling of many ...then used single-molecule FRET to study the intramolecular dynamics of the proteins in solution and when...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...conditions, revealing context-dependent responses and molecular patterns of selectivity. With 10 plasmids available...

... prone to recombination, while a library with a large backbone might be more difficult to transform.  ... per transformation may vary depending on the particular type of cells you are using, as well as other...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in...looking at protocols used for libraries that are similar in size and composition as examples. Table 1 provides...

..., there may be particular poster you are interested in viewing which has drawn a large crowd, making it...don’t fully understand the question, ask for clarification. Having them reframe or elaborate their thought...posters you are interested in viewing. For most larger conferences, poster titles and abstracts are available...permitting, you can go back to the most interesting and popular posters at the end of the session. Get in contact...

...the conference you are attending on the larger side? At large conferences, chances are that several talks... the other person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch... contact information if your conversation is particularly fruitful. After the conference Bring back what...LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation, and you have their ...

...also possess similar morphologies, growth properties, and express stem cell markers similar to ESCs [11...lentiviral infections respectively [9, 10]. These stellar works led to a Nobel Prize for Dr. Yamanaka in ...11].  While iPSCs and ESCs have similar functional utility, iPSCs bypass the transplantation compatibility...2. Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming...

...Medicine. She is particularly interested in science communication and fundamental molecular biology.      ... it. About three or four years into my PhD in Molecular Biology, I realized I wanted a career in science...experience taught me. The article submitted was hilarious, full of interesting information, and received...researchers. It is an excellent way to meet people with similar interests! Especially as someone interested in ...

...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged...discovered - many from viruses, but also some from cellular mRNAs. What they all have in common is the ability...have some disadvantages. These elements are quite large (500-600 bp) and may take up precious space in viral... are short (about 20 amino acids) and produce equimolar levels of mulitple genes from the same mRNA. The...posts or browse Addgene's Plasmid Guide for more molecular biology and cloning information. ...

...actions to a single step. Scorpion chemistry in particular has been reported to work well in the Lightcycler...specifically aimed at determining the best options for a particular cell or tissue type (such as here, here, or here...be worth the initial effort of setting it up for larger or frequently analyzed sample types and sets.   ...analogous Wilcoxon tests have all been shown to give similar results, so you only need to pick your favorite...Real-Time PCR: Methods and Protocols, Methods in Molecular Biology, vol. 1160, DOI 10.1007/978-1-4939-0733... S; Huggett J. qPCR primer design revisited. Biomolecular Detection and Quantification. Volume 14. 2017...

...tools for efficient and programmable insertions of large DNA cargos into bacterial genomes. This new capability...Type II systems (such as CRISPR-Cas9). Unlike in regular Type I CRISPR-Cas systems, however, Cascade from...V-K CRISPR-transposon systems function in a very similar manner to Type I-F, but instead employ a Cas12k...CRISPR-transposons excel at programmable insertions of even very large genetic cargo, at one or multiple desired genomic...systems have already been demonstrated, ranging from large genomic insertions and/or deletions, to multiplexed...

...and injection methods are best suited to their particular scientific question - even when others have already...to successfully replicate published works. Of particular importance to these efforts is the open sharing...Reports are reviewed by Addgene scientists for clarity and accuracy, and by the end of the year, those...Chemically and Functionally Distinct Vectors. Molecular Therapy - Methods and Clinical Development, 18...occurrence during vector purification and formulation. Molecular Therapy, 12(1), 171–178. https://doi.org/10.1016...

...other antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp...synthesis as their mode of action and have a very similar relationship as amp and carb (they are interchangeable...all bacteria used in research, it isn’t a widely popular selection agent. Pros: Stable (spectinomycin). .../1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance to the antibiotic Zeocin...gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-Agar Antibiotic Plating...