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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 662 results for: MAL

Showing: 341 - 360 of 662 results

  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...deliver the best possible videos for free. We’re a small operation, so sometimes it can be a big challenge...premium, so I had to figure out a solution for that. Normally, shots require me to get in close proximity to...good once. Even when things return to relative normalcy, I’ll be incorporating that mobile monitor stand...

  2. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...science, and can include: equipment, reagents, animals, glassware, and even people. When developing a ...budget bottom line costs may be rather high. That’s normal, because this is where you can start to identify... modeling and collaborations rather than using animals, then that’s a cost that you can eliminate from...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...proteoglycan (HSPG)3 AAV receptor (AAVR)2 Epidermal growth factor receptor (EGFR)3 AAV8 Laminin...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

  4. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... will be able to provide letters of reference, informal recommendations, more positive grant application... not know.... 6. Go to science meetings big and small Meetings give you and your research exposure. Use...Eat well- a diet rich in vegetables, foods with minimal processing, and low in empty calories will make...

  5. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...little tissue absorption, weak tissue scattering and small amounts of tissue auto-fluorescence (Drobizhev et...excitation wavelength as it has been shown that even a small incremental change of the excitation wavelength ...Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...Function Antigen presenting cells (APCs) that would normally alert the rest of the immune system to the presence...and IL-10 that suppress APCs. T cells that would normally attack the cancer cells are also suppressed by...activity of CTLs and NK cells. These cells would normally kill cancer cells but are directed not to through...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...choice, and size of the target brain region, the optimal viral titer must be determined empirically (this... weeks over 8 weeks) in order to determine the optimal time period between viral injection and commencement... of neural circuits underlying brain disease in animal models. Nature reviews Neuroscience. 2012;13(4)...

  9. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...Differential cytosine editing between bacteria and mammalian cells Researchers in the Xueli Zhang lab were ...seemed to follow the same initial mechanism as in mammalian cells — by excising the freshly edited U bases...found that C → G editing was often accompanied by small deletions in their target loci (between 4.0% to ...

  10. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This information...differing in the gene/insert. Pooled libraries are normally supplied as liquid DNA with all of the plasmids...

  11. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...gave to me: a miniature CRISPR-Cas system for mammalian use. On the second day of CRISPR, Addgene gave...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...

  12. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid...envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key component...

  13. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...editing and multiplex prime editing (requiring minimal payloads!) Uncovering Ancestral Bias in CRISPR ...Miles on everything from AAV prep to targeting to animal models. It was one of our favorite posts from 2022...

  14. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...started with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly...called, is a critical tool for many scientists. Mammalian cells provide scientists with the means to study...

  15. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...the transition now to being general counsel of a small start-up. For the last 20 years, I was a patent ...very different jobs. Being general counsel at a small start-up is very interesting. It’s very varied. ...

  16. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...References 1. Georgieva, Maya V., et al. "Inntags: small self-structured epitopes for innocuous protein tagging... high correlation for protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed...

  17. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...progressiva (a.k.a. FOP), that, while affecting a small number of people, deserves our attention and has...Plasmid Formats Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection...

  20. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other...
Showing: 341 - 360 of 662 results