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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: Ncl

Showing: 341 - 360 of 628 results

  1. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...antibiotic resistance develop? Microbes (bacteria included) are very skilled at the delicate art of survival... contributing to the development of resistance include (Figure 1): Over-prescription of antibiotics (...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...citing research from outside of their disciplines. Included in the article is an interactive chart where you...is of interest. In his research, Scott Spicer concludes that “… the emergence of the video abstract genre...

  3. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...number of potentially hostile posters (this advice included leaving out the term “implicit bias” which is ...rewriting or coming up with answers, I was able to include links to these posts and eBooks. I also have ...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...and several other pairs have been used since - including: mCerulean/mVenus, mCerulean/Amber, mCerulean/...Other issues that can affect FRET measurements include: the brightness of a fluorophore pair, donor:acceptor...

  5. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...two commonly employed methods for titering IDLVs include detecting vector particle components or performing...Bayford, J., Brown, L., Quaye, M., Kinnon, C., Ancliff, P., Webb, D. K., Schmidt, M., von Kalle, C., Gaspar...

  6. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...easy to follow seminar See item 2. Attendees will include grad students (that are solicited for feedback)...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...

  8. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...Build: Jennifer created a mobile camera area that included iPad and her laptop raised on an ironing board... the camera, to control some basic functions, including starting and stopping recording).  Test: It was...

  9. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...ideal model organism to study an array of topics including development, genetics, and the nervous system.... an array of biological systems and processes including the nervous system, development, cell signaling...

  11. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...While the biology behind this AAV effect remains unclear, it is likely not a coincidence that the AAV genome...past fifteen years. Some examples from their lab include using AAV to introduce epitope tags into the endogenous...

  14. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...attached to the active site of key metabolic enzymes including biotin carboxylase and decarboxylases. These enzymes...oligonucleotides can be used in a multitude of applications including but not limited to in situ hybridization, blotting...

  15. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...if some of your work does relate, make sure to include that material, but don’t force it. You can always... Having answer slides ready after your formal conclusion slide will also help with your confidence. It...

  16. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications,...pSer-incorporated proteins on a single plasmid. This includes 4 copies of a tRNA that specifically recognizes...

  17. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... search suggests there are hundreds. This only includes general lab strains designed for subcloning or...or protein expression. If you were to include customized strains, the number is probably in the thousands...

  18. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...plasmid sequencing services to verify the plasmids, including long-read sequencing techniques, such as Oxford... presence of a multimer in your sample remains unclear after visualizing it on a gel, one of the other...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... variants that are less sensitive to ethanol, including the “middle” ADH1 promoter pADH1m, which maintains...3 in a single modular vector for ease of use! Conclusion This post contains only a brief overview of some...

  20. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...with minimal cross-reactivity. Other strategies include using engineered ligases to attach a ligand to ...choosing a dye or ligand to use for your experiment. Conclusion Researchers and vendors continue to develop new...
Showing: 341 - 360 of 628 results