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We narrowed to 837 results for: RING

Showing: 341 - 360 of 837 results

  1. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...on how they've managed to keep themselves happy during their time in grad school. As you'll learn, grad... hard about picking a good mentor, and begin considering career options early. Tune in for great advice... in grad school? As part of this discussion, we bring up a performance Ben and Niroshi organized for Tyler's...Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and Ben for taking...

  2. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...can help with plasmid sharing! Judging by how many prizes were given out during the lunchtime pizza and...see a real working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite... that are offered with a biology major.” After hearing about many Addgenies’ career paths, Michelle Healy...

  3. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...articles appearing in lower-prestige journals has risen steadily from 1995 to 2013. Considering citations...publications alone aren’t enough. Scientists are sharing what they call “raw science” – data sets, code,...researchers to track and share their own libraries and offering another way to consider and quantify the impact...

  4. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that...haven’t already used it yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR...work probing mTOR signaling in cancer cells, deciphering the mechanism of matrix metalloproteinase-9 secretion...Addgene and look forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale...

  5. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    Published
    July 7, 2017, 1:32 p.m.
    ...focused on our core mission to promote scientific sharing. Listen to learn more about Darrell, his research...repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary nature of the Center...practices and promotes open science in the lab - sharing reagents, cell lines, data, and knowledge without...Addgene board of directors - increasing open source sharing and helping us stick to our core mission. Chapter...

  6. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...cells using flow cytometry, you are typically measuring the presence or absence of certain markers on ...photon is emitted. This step is called emission. During excitation, some of the photon's energy is lost... red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation spectra around...you can see in Figure 1, emission spectra of neighboring colors can and do overlap. For example, APC overlaps...hypothetical experiment in the lab. Let's say I am culturing HEK 293T cells in vitro. I transduced the cultured...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...in order to directly show that, for example, the firing of a specific type of neuron in a specific brain...whether you wish to stimulate or inhibit neuronal firing, the cell-type specificity, the kinetics of the... which results in depolarization and subsequent firing of an action potential. Halorhodopsins (HR) are...may more effectively silence neurons than HR. Delivering your opsin  The standard opsin delivery method...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

  8. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an... experiments. Addgene’s dedication to resource sharing has led us to develop a searchable and sortable...can search by any part of the data. For example, entering ‘C. elegans’, or ‘elegans’ will filter the table...CRISPR Technologies Available Through Resource Sharing. 2016. In press. Additional Resources on the Addgene...

  9. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...unmatched customer support, as this is vital to ensuring that our services help accelerate your research...support team that can help you through any search, ordering, technical, or quality control issues. Improving...within the widget, you’ll navigate to articles answering your questions making it easier than ever to get...describe your issue and press send. Doing so will bring your concern directly to our support team’s attention...

  10. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ..., Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating...normally and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing...and relevance for the efficiency of BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva... Mary Gearing...

  11. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...success.” 2. “Enjoy Teamwork.” Be friendly, open, sharing, rather than intensely competitive. Be curious ...society. We need much, much more science and engineering (STEM) expertise in law, business, journalism...connected to scientists and use the skills they learned during their studies. They suggest that you make time ...Connie Cepko, Tom Ellis, and George Church for answering our burning questions about how they manage and...

  12. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...CASTs quickly became popular bacterial genome engineering tools, with early reports of 40%–60% efficient... & Sternberg, S. H. (2024). Bacterial genome engineering using CRISPR-associated transposases. Nature ... Berríos, K. N., McElroy, A. N., King, R. T., Stringham, O. G., Gelsinger, D. R., Vo, P. L. H., Chen, ...the Addgene blog INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Degrading DNA with ...

  13. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...stage you could infer that the gene which GFP was monitoring was being expressed in the same pattern. These...result but will still allow for expression pattern monitoring. Enhancer traps Like promoter traps, enhancer...unambiguously identify the gene that they are monitoring as they are directly integrated into it. Enhancer...e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007...

  14. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...can remain present until cleared by the body. Administering the drug once can result in several hours of... Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to...injection. The main challenge in using ligands is ensuring the agonist is able to cross the blood brain barrier...Bryan Roth lab for feedback on this article and sharing their expertise with us. References Urban DJ, Roth...

  15. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...hydrogen bonds, destabilizing the molecule and favoring the separation of the two helixes. Low pH instead...chromosome. One of the main causes of degradation during plasmid DNA extraction and purification from E....though in their capacity to maintain DNA stability during storage over prolonged periods of time, especially...especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should consider a couple...

  16. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...grow. Auxotrophy and prototrophy come into play during cloning if you’ve designed your experiment using...sucrose. Plasmid cloning: Creativity with naturally occurring genes  At this point in the blog post, you may...negative selection strategies adopt naturally occurring genes for cloning purposes (antibiotic resistance... 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection Resources...

  17. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to stabilize...stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps capture...reducing the read depth necessary when sequencing and lowering the amount of tissue needed per experiment. In...transcriptional regulatory elements is controlled during development using the Drosophila wing as a model...

  18. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ... category already has five blue flame plasmids covering three different epigenetic effectors. The diversity...Bacteria: CRISPR Methods for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing...CRISPR History and Development for Genome Engineering  ...  Mary Gearing...

  19. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    Published
    Oct. 3, 2017, 12:11 p.m.
    ...speaker biographies.” During the week of our interview, Hans was also busy preparing materials for an upcoming...took that gamble, and it’s paid off. Work is never boring. “I just keep learning on the job. It keeps it ...mechanism that also looks nice is a great way to bring people in.” Are you interested in careers outside...

  20. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Vandenberghe. We’re excited to grow the team and bring on scientists with a diverse set of experiences....scientist that helps with different aspects of manufacturing and quality, especially with regard to compliance...journey in biochemistry made the foray into genetics during graduate school at MIT. “I read some of the seminal...He’s also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...
Showing: 341 - 360 of 837 results