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Showing: 341 - 360 of 619 results

  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...lecture at iBiology. Commonly used filter sets & relevant fluorescent proteins Commonly used filter sets...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...bacteria, plasmids used for stable transfections rarely contain an ORI since the integrated DNA will be... Stably transfected cells may be considered an entirely new cell line from that of the original parental...

  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...protocol link). We have found this method to be reliable and reproducible. However, we do not use amplicons...which does not a standard curve and somewhat more reliable quantitation. Differences in sample treatment ...

  4. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...used to indicate them on the map, direction (if relevant), and types. Types listed are standard GenBank...Addgene software development teams for working tirelessly to implement these improvements. Let us know ...

  6. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection...

  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR...mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan Roth’...

  8. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...by the bacteria after transformation. LIC is a reliable cloning method, but it is limited by its sequence...SLIC), eliminated many of LIC’s constraints by relying on the power of homologous recombination. SLIC ...

  9. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...are some of the AAV and lentiviral preps we have released in the last few months: Lentiviral preps for ...coming soon! These vectors listed below should be released at Addgene in the next 6 months, pending quality...

  11. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...heat shock. In the case of Hsp70, the heat shock releases heat shock factor 1 (HSF-1), which subsequently...cells and typically only in the same or closely related species from which they were derived. Similarly...

  12. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...polymerase. E4/E1A activate transcription of other relevant viral genes (including the ones listed previously...

  14. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...cannot convince others that it is important and relevant to them.   When you think about it, virtually ... to forget that you have a much bigger job than merely telling your audience about the work you've done...

  15. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...researchers in creating and sharing knowledge, related to the types of materials we help distribute and...had the deeper expertise of our antibody team to rely on. High-impact content like the antibody guide ...

  16. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...specific goal for each analysis will help you locate relevant information in the future. Approach: Outline...can store any power point, excel, or other files relevant to the project. README: The README.txt document...

  18. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... and how these pathways work, as this could be relevant when planning your experiment. Here, we’ll discuss...repair is that the Cas9 protein doesn’t immediately release from the broken end after cleavage, which may interfere...

  20. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...biology. Plant molecular biology has traditionally relied on overexpression and knockouts to better understand...technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full of potential...
Showing: 341 - 360 of 619 results