We narrowed to 479 results for: Ret;

1. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

2. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

3. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ...

4. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and is thus HEPN-independent. These...

5. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ..., does not require standard curves, and can theoretically detect a single target molecule in a sample....

6. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...instead of incorporation via plasmid or media. Theoretically, if you put all these together, the cell will...

7. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   ...incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs (right ...

8. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically not feasible...

9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated...

10. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

11. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...most experienced users and puts it in the hands of pretty much all investigators. Our kit makes it extremely...

12. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...

13. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ...able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going...

14. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal...

15. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...

16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...

17. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction...

18. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    Published

    April 10, 2018, 12:56 p.m.

    ... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...

19. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...grad school with the plan of being a scientist. I pretty quickly figured out that I just don’t have the ...

20. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...on Addgene.org Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids...