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We narrowed to 847 results for: STI;

Showing: 341 - 360 of 847 results

  1. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...microscope designs may further exploit the unique characteristics of fluorophores to block out of focus fluorescence...for a new sample I always start by asking two questions: 1) Is the sample dynamic or static? 2) Is the...the sample thin (< 15 μm) or thick? These questions will place each sample into one of four categories ...blurry out-of-focus light. The most difficult characteristic to capture in these types of samples is their...from a well-established microscopist at your institution when heading into a new set of experiments. ...

  2. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...proteins, so you can't distinguish the different proteins from each other. To distinguish different target ...inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...fluorophore? Strong brightness makes it easier to distinguish a signal from noise; therefore, it is recommended...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

  3. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...representing core nodes that, when over-expressed, constitutively activated or inactivated the pathway. “The ...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs...

  4. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...Maintaining Institutional Knolwedge and Continuity - SITN's key technique for retaining institutional knowledge...mosquitoes, allergies, & more! We briefly answer the question, will the effects of climate change make put Cambridge...junior and one senior.  To learn more about the STIN's podcast, email [email protected]    For more...

  5. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ... Twitter.” This paper addresses the following questions about scientists on Twitter: who are they? What...they are based on the US Occupational Employment Statistics (OES), but international Twitter users were included...orange)  scientists largely cluster by discipline, suggesting that scientists tend to follow scientists within...out the Top 50 Science Stars on Twitter Want suggestions on how to use social media as a scientist? Check...

  6. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...                           National Institute for Physiological Sciences Broad Institute                                       ...Hospital                           California Institute of Technology             Johns Hopkins University...Academy of Medical Sciences University of Texas at Austin University of California, San Francisco  University...

  7. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ... the panelists were interesting enough to make up for the somewhat naïve questions. During the panel, ...their seminal, cooperative paper in Science.  Interestingly, this project started with their labs studying...for billions of years (…) Bacteria are really sophisticated”.     New to CRISPR/Cas9 Technology? Learn ...

  8. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...14] Hepatocytes [15] Pancreatic β-cells [16] Intestinal tissue [17] Lung epithelial cells [18] Red ...approaches differ in several ways. Below is a table listing some of critical differences (adapted from Zhou...Zhou and Melton, 2008, [43]): Characteristic Reprogramming+ Transdifferentiation in vivo or in vitro...differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 2011. 470(7332): p. ...37. Merrell, A.J. and B.Z. Stanger, Adult cell plasticity in vivo: de-differentiation and transdifferentiation...

  9. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...mitigating noise generated by off target digestion. After MNase digestion, fragments are released from nuclei... DNA around the target protein. This targeted digestion is controlled by the addition of calcium, which...measure of DNA binding sites in combination with existing ChIP-seq data sets, of which there are many readily...

  10. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...property by including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is flanked... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...sites. To introduce a gene of interest into the destination plasmid, recombination sites must first be added...

  11. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly species that jump... is a lot of excitement around using sdAbs in diagnostic applications. For example, fluorescently and ...generate new sdAbs. There has also been some interesting work done developing transgenic mice that express...S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https...

  12. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ... fastest way for us to release your plasmids is still for you to notify us as soon as your article is ...a project isn’t working out as planned, but you still hope somebody will find the plasmids useful, we’...article, a preprint, or without any paper at all. Posting preprints and openly sharing materials are both...think. We appreciate your feedback. If you have questions about depositing, check out our website, Help ...

  13. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... some special characteristics of scientists in the work place as described by Sebastiano Massaro in his...some of these common pitfalls of management and still not get the most from your team. It is pretty clear...learned to get results with the carrot, and not the stick.     This is the first in a 5 part series on Introduction...

  14. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...are on the right track if they start asking you questions. Remember that people like a good story and science... this case you – on a quest to ask and answer questions and hopefully discover something new. Science ...out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ...

  15. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...Makarova et al. grouped the systems into three distinct types and subtypes using polythetic classification... not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between...viral anti-CRISPR genes. These data highlight an interesting "arms race" between phages and prokaryotes; the... and further develop the CRISPR/CAS toolkit by posting generic lab protocols, providing tips from experts...

  16. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... mountain – with many routes up and only one destination – a career in the sciences is more like an intergalactic...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual... weird and rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination...with things like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance...

  17. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ... have the potential to provide improvements to existing technologies based on polymerase processivity....stable, highly soluble, and non-immunogenic yet are still just as specific to their target as regular antibodies...podcast segment Steven Carr’s lab at the Broad Institute focuses on proteomics: developing and applying...analysed. In this work, the authors specifically investigated protein binding at the TERT gene, but this method...

  18. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...University, and the Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition...Nathan Shaner and colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...

  19. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...were unable to deduce the mechanism responsible we still believe that these results will be beneficial to...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...

  20. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies...Patrick says researchers can ask themselves a few questions to get started: Will the plasmid be used exclusively...that plasmids with low to medium copy numbers can still express massive amounts of protein given the proper...cells have not started to die off. Have any questions? Need something clarified? If so please, post ...
Showing: 341 - 360 of 847 results