We narrowed to 611 results for: STO;

...-3.13. To test xCas9 3.0-3.13, Hu et al. first restored catalytic residues D10 and H840. To ensure that...Plasmids for Mammalian Systems Learn about CRISPR History ...

...Each mRNA molecule in the library doesn’t encode a stop codon, which causes the ribosome to pause at the... Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann...

...levels. Chromoproteins in nature Compared to the history of fluorescent proteins in molecular biology research...research, chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored...

...late 1960s during research at hot springs in Yellowstone National Park.  In addition to Taq DNA polymerase... low during the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction...

...restriction sites allowing you to easily create customized overhangs to assemble up to 6 different fragments...PCR process (the precise mechanism isn’t well understood). Finally, TOPO cloning is another good option...

...to tailor your presentation so that it can be understood by your audience. Use your poster as an aid: ... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often...

...HDR. If the max CFD score is less than 0.03, it stops mutating. (This happens often because the HDR inserted...will check the CFD score. If it is below 0.03, it stops mutating. (Note: the most common synonym in the ...

... skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies have shown that AAV11...purification, and titration. Can't wait? Browse our In-Stock AAV inventory or use our Packaged on Request service...

... cleaving of the target strand is not as well understood but likely occurs in similar regions. This combination...up to hundreds or even thousands of base pairs, stopping either at boundaries defined by anti-CRISPR proteins...

...particles you need are not available, or the cost of custom viruses are too high for your budget. In those ...round provides the added benefit of producing a stock P1 virus, so that if you ever need to remake the...

...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

...see our plasmid collection from Sue Jaspersen (Stowers Institute) and Mark Prescott (Monash University...ImageJ plugins JACoP and Coloc 2, BioImageXD, and customized CellProfiler pipelines. Image analysis software...

... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid, and...

... was contributed by guest blogger Erik L. Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple...spiderweb-like pattern of tubules can be grossly distorted into dense stacked membranes termed Organized ...

... cassette into a given locus, just upstream of a stop codon. Briefly, the PITCh vector should be constructed...the GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro ...

...would be useful for their own research, and helps requestors use the plasmid in their own labs. We highly ...survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such as some...

...that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).  CLARITY is...236.
 Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken...

...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...Addgene's new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our...

..., M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression from a replicating...PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. J., & Wold, W....

...some integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org... Tissue Fixation & the Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com...