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Showing: 341 - 360 of 598 results

  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...Each mRNA molecule in the library doesn’t encode a stop codon, which causes the ribosome to pause at the... Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann...

  2. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...levels. Chromoproteins in nature Compared to the history of fluorescent proteins in molecular biology research...research, chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored...

  3. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... cleaving of the target strand is not as well understood but likely occurs in similar regions. This combination...up to hundreds or even thousands of base pairs, stopping either at boundaries defined by anti-CRISPR proteins...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...particles you need are not available, or the cost of custom viruses are too high for your budget. In those ...round provides the added benefit of producing a stock P1 virus, so that if you ever need to remake the...

  5. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...late 1960s during research at hot springs in Yellowstone National Park.  In addition to Taq DNA polymerase... low during the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction...

  6. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...to tailor your presentation so that it can be understood by your audience. Use your poster as an aid: ... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often...

  8. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid, and...

  11. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... cassette into a given locus, just upstream of a stop codon. Briefly, the PITCh vector should be constructed...the GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro ...

  12. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...would be useful for their own research, and helps requestors use the plasmid in their own labs. We highly ...survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such as some...

  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).  CLARITY is...236.
 Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken...

  14. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...Addgene's new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our...

  15. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ..., M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression from a replicating...PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. J., & Wold, W....

  16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...some integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org... Tissue Fixation & the Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com...

  18. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...to late-career grant professionals have similar stories about a time when their passion for an organization...their new career path.  A part of the career path story for most established grant professionals is learning...

  19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...including MTA and shipping. Addgene realizes the One-Stop shopping for a wide range of plasmids. The merit... Their progress might be slow, but if they don't stop, they may be able to achieve something that may ...

  20. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...career solving hard problems or who needs a stepping-stone to many other opportunities. Many thanks for our...Bioengineering and works as a healthcare consultant in Boston. He is interested in the business of life sciences...
Showing: 341 - 360 of 598 results