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We narrowed to 738 results for: TRI

Showing: 341 - 360 of 738 results

  1. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...default scoring matrix BLOSUM62, but you may want to check the description of the other matrices to see if ...laboratories that deposit their plasmids with us or other entries in NCBI’s numerous databases. As our repository...

  2. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...production, reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

  3. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...and relatively easy? Have all of these things contributed to the steadily increasing distrust the general...that “Addgene, with its easy, fast, and cheap distribution of the newest CRISPR-Cas plasmids, has been ...to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino...

  4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...sgRNA-level metrics to assess library performance in viability screens. Based on these metrics the Brunello... CRISPRi libraries with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant...

  5. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger, Luke Lavis, a Group Leader at the Janelia Research Campus...synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly capable of synthesizing... D. Lavis! All images in this blog post were contributed by Luke D. Lavis. Luke D. Lavis is currently ...

  6. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...therapy. In 2003, VIRxSYS began the first clinical trial with a lentiviral vector. CD4+ T-cells from patients... lentiviral vectors have been observed in small trials for other diseases, including the common hematopoietic...monitoring and follow-up in lentiviral clinical trials. McGarrity GJ, Hoyah G, Winemiller A, Andre K, ...

  7. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish...you’re screening for positive clones! Tips and tricks from the bench Don't pick too large of a colony...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly...

  8. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...competitions (ranging from cook-offs, to corn toss to trivia, and everything in between). Eleven years later...everything from kayaking, to field day, to relay triathlons, to 5Ks. Many of these examples we’ve done annually...are the sole event planner)! If you have a very intricate itinerary, there is a higher likelihood that something...

  9. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...specific DNA sequences. Thus, we've made some contributions in technology development in biology and hope...in these professional activities because I can contribute by, for example, helping to improve the papers...optimized for living for Japanese, which is not trivial, and the grant situation is currently much better...

  10. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... mYFP S65G; V68L; Q69K; S72A; T203Y; A206K Citrine S65G; V68L; Q69K; S72A; T203Y ECFP F64L; S65T...Fluorescent Protein Guide Note: A. Max Juchheim contributed to the writing of this article. ... Marcy Patrick...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... the nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells... fluorescent proteins, they found that adding a triple nuclear localization signal or including fusions...CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward loop. ...

  12. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...This post was contributed by Joe James from Binning Singletons. The sheer scale of a large conference...their meetings. Amanda Gunn ran randomized coffee trials for us at ASCB|EMBO 2019, matching Mentors with...ways. Scientific societies and conferences should strive to provide support to early career scientists, ...

  13. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...This post was contributed by Rory Macneil, founder of Research Space. There are many types of electronic... the ELN is reproducible, and only the highly restricted number of people who can access the ELN have ... the ELN arises here. Notwithstanding access restrictions based on permissions, however, in some cases...

  14. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...This post was contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State...strand, dramatically reducing the likelihood of detrimental indel formation. After binding with DNA, the ...editing causes the creation or destruction of any restriction enzyme (RE) recognition site, RE analysis of ...

  15. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ... binding signal for Cas9 and this sequence is a strict requirement for Cas9-mediated DNA cleavage. The...below. Note: Jennifer Tsang and Mary Gearing contributed to the update of this post. References Abudayyeh...Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome...

  16. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field...success. Or the client might need help with the intricate task of designing a strategic vision for a portfolio...styles, team situations, and personalities. Teams striving to achieve a lot of great output in a short amount...

  17. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle and Alex Chavez...activation can be made inducible upon environmental triggers. This is done by creating a split dCas9 protein...activation are also reversible: once the environmental trigger leaves, gene expression is no longer activated....

  18. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... This post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate...position requirements. For example, a KDEL ER retrieval sequence only functions at the extreme C-terminus...the fluorescence pattern is equivalent to the distribution of FP-fusion proteins. Our studies and those...

  19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...lentiviral vectors, the lentiviral genome is distributed across multiple plasmids. For simplicity, we’...settings, as evidenced by retroviral gene therapy trials for X-linked severe combined immunodeficiency (... (SCID-X1) in the early 2000s. These trials unfortunately resulted in several patients developing T-cell...

  20. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...committees. Having successfully navigated the trials and tribulations of the process, I’ve provided guidance ...ability to interview and sell a vision for your contribution to a Department's/Institution's success is the...
Showing: 341 - 360 of 738 results