...the insert. Gateway® technology (developed by InvitrogenTM) is essentially a more modern version of this...teachline.ls.huji.ac.il/72682/tutorials/gateway/introduction.html demonstrating the principal behind the “swapping...CcdB-resistant strain is ccdB Survival™ from InvitrogenTM. Using either DB3.1 or ccdB SurvivalTM, one ...PubMed PMID: 1324324. 6. Tam, JE. and Kline, BC. “Control of the ccd operon in plasmid F.” J Bacteriol. 1989...

...AAV (AAV-pgk-Cre) has been used to successfully introduce Cre into cells of interest. What if I need two...of using loxP sites is the inability to tightly control which loxP sites recombine if more than two are...allowing for two separate recombination events to be controlled in parallel. Have you used the Cre-lox system...PubMed Central PMCID: PMC49604. 4. Independent control of immunoglobulin switch recombination at individual...

...live cells.  Controls  When designing experiments, it’s important to include proper controls to assess the...quantitative readout of the interaction in vivo or in vitro, and can be used for measuring protein expression... p53 tetramerization in response to DNA damage controls the p53 response. Gaglia G, Lahav G. Mol Syst ...

...chemogenetics? Chemogenetics relies on genetically introducing engineered receptors that are only activated ...Expression Using chemogenetics requires successfully introducing receptors to target cells. Targeted and specific...mitigated by using low doses and running appropriate controls (Jendryka et al., 2019), they have resulted in...channel, the PSAM acts as a gating mechanism to control ion flux. The system allows you to directly manipulate...

... proteins. The Seeger lab developed a robust in-vitro protocol that enables rapid selection of synthetic...sybodies that hypothetically contain ones that bind strongly to your target protein. The Seeger lab designed...selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each mRNA molecule...enrichment step for identifying sybodies that bind strongly and specifically to the protein of interest. The...

...of interest to better study them in vivo and in vitro, especially in the mid-late 20th century with the...reticulum localization, Munro and Pelham casually introduced the Myc-tag (Munro, et al. 1986; Munro and Pelham... and the Myc-tag today Since Munro and Pelham introduced the Myc tag to the world, it has become one of...this antibody works and allow users to better troubleshoot and design experiments. It turns out that even...

...can be exploited to either inactivate a gene or introduce a desired genetic alteration. Listen to Our Podcast...necessary gRNAs, but it would not be possible to control which gRNA becomes complexed with which variant...represents a straightforward means of exerting tight control over Cas9 nuclease activity while eliminating the...guides targeting one set of genetic loci can be introduced together with truncated gRNAs targeting a different...

...advantages degron tags can offer over other protein control methods and compare the commonly available types...etc. Firstly, they are reversible mechanisms of control. Protein degradation can be induced by the addition... Tsien RY, Lin MZ. Tunable and reversible drug control of protein production via a self-excising degron...  Susanna Stroik...

...experiments attempting to build gene drives in tropical mosquito vectors of diseases such as malaria and...then, using multiple confinement strategies is strongly advised. The effects are multiplicative, and there.... Site-specific selfish genes as tools for the control and genetic engineering of natural populations....Fitz-Earle, M., Holm, D. G. & Suzuki, D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome...

...and would avoid genomic off-targets or indels introduced through non-homologous end joining (NHEJ). Designing...recapitulate or rescue known pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional...also adjusted the structure of the guide RNA by introducing a C opposite the target A to be edited. This ...off-target edits around the targeted nucleotide. Introduction of guanine-mismatches in the guide across from...

...for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work/life balance....possible to bring all aspects of our lives under control? Balance is a myth and should not even be the real... don’t have children, so my life will be under control.” Think again. Maybe you’ll need to care for an...to do most tasks – try to resist the impulse to control by insisting things always be done your way. Be...

... Martin et al., 2018). The ACE reporter is a bicistronic construct that consists of a mutated mCherry ...eGFP. To create the inactive mCherry, the lab introduced a 43 base-pair insertion that disrupts fluorescence...use across different cell types and systems. To introduce flexibility, the Dow lab created a base editing... optimized cytidine base editor. The lab then introduced an sgRNA targeting the GFPACG to the cells. As...

...founder of Research Space. There are many types of electronic lab notebooks (ELNs), each with its own pros ... virtue of liberating data from paper into an electronic environment and hence making it searchable and... of Research Space, which provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts...on the Addgene blog Find tips on choosing an electronic lab notebook Watch a livestream of the Minisymposium...

...protocols therefore differ from library to library. We strongly recommend following the protocols provided by ...a low multiplicity of infection (MOI). MOI is controlled by viral titer, so you’ll need to measure your...Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend determining the functional...transduction protocol you can follow and a troubleshooting blog should you run into any issues.  Keep ...

...and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis (those..., Akhtar and Marty, Jean L. “Aptamer based electrochemical sensors for emerging environmental pollutants...systems using immobilized aptamers.” Biosens Bioelectron. 2011 May 15;26(9):3725-36. PubMed PMID: 21419619...FRET in Your Experiments Learn more about GFP Controlling Protein Activity with GFP Resources at Addgene.org...

...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter is...job with a lot of file management that asks for strong organizational skills. You might think, “I’d be...letter The opening paragraph is the place to introduce yourself and your background. It’s a great place...and is important for the job]. Notice that the intros above allow you to highlight what specific skills...

...social sciences go uncited. Creative titles and controversial topics can garner some attention, though in ...Journal of Number Theory (1,519 views), and Gastroenterology (1,046 views) rounded out the top four average...infections. The video abstracts provide a brief introduction to the research and, after watching the video...links to the videos, keeping the copyright and control over the videos with the researcher and the host...

...overexpression vector rather than going through all the troubleshooting required for Cas9-based activation. CRISPRa...Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ (2019...Weissman JS (2014) Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647...2015) A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11...

...Level 1 microbe makes it a perfect candidate for introductory teaching and research environments (Idalia &...study human cardiovascular disease, specifically stroke and hypertension (Iannaccone et al., 2009). Rodents...Resource, a breeding facility for X. laevis and X. tropicalis, the two most commonly used species of frog. ... study human disease-relevant genes in such a controlled environment displays how essential the frog is...

...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...plasmids encoding aptamer-tagged RNA and later introducing the small molecule into the cell. The fluorescence...metabolites, leading to conformational changes that control the expression of coding RNA. Spinach-based riboswitches...then identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity...