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We narrowed to 393 results for: ara-2

Showing: 341 - 360 of 393 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...the necessary steps to perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline...QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow   Over the years we have...pipelines, including bioinformatics. We loved the parallels between Seqera’s mission and our own, as both ...the sequential tasks in our pipeline, we wrote a separate Nextflow process. In Nextflow, a process defines...concept of channels and implemented the input declaration correctly, the script block in the second process...

  3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...You’ve probably heard that only 2% of our genome is made of protein-coding genes, and you might be wondering...transcriptome engineering References Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A Programmable...CRISPR-Based Approaches for the High-Throughput Characterization of Long Non-Coding RNAs. Non-Coding RNA. 2021...

  4. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...overview of these new variants:   Figure 2: Inner mitochondrial membranes imaged with structured...and screening that led to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco... Endothelial-specific AAV delivery By Cythina Arokiaraj (Gradinaru lab) Another useful viral tool deposited...

  5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...) 12 (>100) xCas9(3.7)-ABE 69 (1.4) 43 (2) 21 (3) Not examined 16 (>100) Average percent...xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian cells Hu et al. compared...SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide off-targets, since increasing PAM...

  6. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ... Werner S, Marillonnet S. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PubMed...few new ones that are not used so much but have comparable expression levels to things people do use a lot... developed mostly with model plants in mind, Arabidopsis and Nicotiana benthamiana. N. benthamiana is ...

  7. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...strain (which will be covered in more detail in part 2 of this blog series) controls the cell types in which...derived from random crosses between mice of uncharacterized backgrounds to create a limited but genetically...

  8. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...company culture.  It’s also a good idea to prepare 1-2 unique questions for each interviewer by looking at...of the theme of my interview stories as the characteristic which the story demonstrates I possess.   Bullet...

  9. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...of these issues are not particularly new. Chapter 2, “The Professional Scientist,” addresses the challenges...over two decades, attempts to answer it in five paragraphs, with examples. If that sounds ambitious, that...them wanting to learn more. There are individual paragraphs that may have warranted their own chapters, and...

  10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct...or diluted as the cell grows resulting in the appearance of unmethylated sites in the chromosome. The ... without plasmid addiction systems. For example, Cara Morin and James Kaper used the hok/sok system to...223.1 (1992): 41-54. PubMed PMID: 1370544. Morin, Cara E., and James B. Kaper. "Use of stabilized luciferase-expressing...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...al (Nature. 1988 Jul 28;334(6180):320-5.) PubMed. 2. A segment of the 5' nontranslated region of encephalomyocarditis...multicistronic vectors simultaneously express two or more separate proteins from the same mRNA. We've discussed promoters...at the C-terminus of a 2A element, leading to separation between the end of the 2A sequence and the next...

  12. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...that numbers are not switched around or misread.  2. Track the container - It is just as important to ...to fulfill orders.  The barcoded tubes help to guarantee that no samples get lost during their journey....Addgene Get help with plasmid transformation, preparation, and analysis from our protocols and molecular...

  13. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...PMID: 18478103. PubMed Central PMCID: PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging ...PMCID: PMC552224. 4. Gustafsson, Claes, Sridhar Govindarajan, and Jeremy Minshull. "Codon bias and heterologous...: 346-353. PubMed PMID: 15245907. 5. Maertens, Barbara, et al. "Gene optimization mechanisms: A multi‐...

  14. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that encodes...https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous...3645–3650 https://doi.org/10.1073/pnas.0909910107 Varani G, McClain WH (2000) The G·U wobble base pair. ...

  15. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells." State University...line identity and purity making it easier for comparable studies to be performed on a cell line even decades...preferably some of these vials will be stored in a separate backup liquid nitrogen store to prevent loss from...

  16. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...unc- 119 + selection." MicroPublication 1 (2018): 2–3. Wang, Han, et al. "cGAL, a temperature-robust GAL4...sequencing of neurons from each class to fully characterize the transcriptome. The first data release for...

  17. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...non-specific binding in our IHC condition.   Fig. 2: Schematic of the goldilocks antibody selection process...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens when...

  18. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    .... Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed...the project were able to move forward quickly in parallel. More broadly, we drew on the combined creativity...

  19. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO...intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels of the second messenger... a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence is observed when these...23488798. PubMed Central PMCID: PMC3775879. Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin...Griffin. "Development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2...et al. "Ribonucleoprotein purification and characterization using RNA Mango." RNA23.10 (2017): 1592-1599...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...Thomson, M., & Gradinaru, V. (2021). Deep Parallel Characterization of AAV Tropism and AAV-Mediated Transcriptional..., with significant contributions from Cynthia Arokiaraj. Addgene would also like to thank Viviana Gradinaru.../doi.org/10.3389/fimmu.2021.730825 Campos LJ, Arokiaraj CM, Chuapoco MR, Chen X, Goeden N, Gradinaru V...Adams, C. D., Yang, D., Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V., Campos, L. J., Griffiths, J. ...
Showing: 341 - 360 of 393 results