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We narrowed to 811 results for: lars

Showing: 341 - 360 of 811 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...pathways and represent the largest family of human drug targets. To enable large-scale profiling of many ...then used single-molecule FRET to study the intramolecular dynamics of the proteins in solution and when...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...conditions, revealing context-dependent responses and molecular patterns of selectivity. With 10 plasmids available...

  2. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ... prone to recombination, while a library with a large backbone might be more difficult to transform.  ... per transformation may vary depending on the particular type of cells you are using, as well as other...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in...looking at protocols used for libraries that are similar in size and composition as examples. Table 1 provides...

  3. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...Medicine. She is particularly interested in science communication and fundamental molecular biology.      ... it. About three or four years into my PhD in Molecular Biology, I realized I wanted a career in science...experience taught me. The article submitted was hilarious, full of interesting information, and received...researchers. It is an excellent way to meet people with similar interests! Especially as someone interested in ...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged...discovered - many from viruses, but also some from cellular mRNAs. What they all have in common is the ability...have some disadvantages. These elements are quite large (500-600 bp) and may take up precious space in viral... are short (about 20 amino acids) and produce equimolar levels of mulitple genes from the same mRNA. The...posts or browse Addgene's Plasmid Guide for more molecular biology and cloning information. ...

  5. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...actions to a single step. Scorpion chemistry in particular has been reported to work well in the Lightcycler...specifically aimed at determining the best options for a particular cell or tissue type (such as here, here, or here...be worth the initial effort of setting it up for larger or frequently analyzed sample types and sets.   ...analogous Wilcoxon tests have all been shown to give similar results, so you only need to pick your favorite...Real-Time PCR: Methods and Protocols, Methods in Molecular Biology, vol. 1160, DOI 10.1007/978-1-4939-0733... S; Huggett J. qPCR primer design revisited. Biomolecular Detection and Quantification. Volume 14. 2017...

  6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...tools for efficient and programmable insertions of large DNA cargos into bacterial genomes. This new capability...Type II systems (such as CRISPR-Cas9). Unlike in regular Type I CRISPR-Cas systems, however, Cascade from...V-K CRISPR-transposon systems function in a very similar manner to Type I-F, but instead employ a Cas12k...CRISPR-transposons excel at programmable insertions of even very large genetic cargo, at one or multiple desired genomic...systems have already been demonstrated, ranging from large genomic insertions and/or deletions, to multiplexed...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...and injection methods are best suited to their particular scientific question - even when others have already...to successfully replicate published works. Of particular importance to these efforts is the open sharing...Reports are reviewed by Addgene scientists for clarity and accuracy, and by the end of the year, those...Chemically and Functionally Distinct Vectors. Molecular Therapy - Methods and Clinical Development, 18...occurrence during vector purification and formulation. Molecular Therapy, 12(1), 171–178. https://doi.org/10.1016...

  8. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...other antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp...synthesis as their mode of action and have a very similar relationship as amp and carb (they are interchangeable...all bacteria used in research, it isn’t a widely popular selection agent. Pros: Stable (spectinomycin). .../1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance to the antibiotic Zeocin...gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-Agar Antibiotic Plating...

  9. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...enhancer it targets is a “pillar of function” for our stem cells. Remove this pillar and the ESC dies. Order...knowledge of which stretches of the genome are pillars for stem cells, or metastasized tumor cells, or... a double helix. People are wrong. In reality, cellular DNA is a complex three-dimensional globule of ... 10 meters apart, are mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function, they found that...found two that display robust cleavage activity similar to that of Cas9. These two nucleases, AsCpf1 and...respectively), both cleave in a staggered pattern similar to FnCpf1. Potential advantages of Cpf1 over Cas9.... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

  11. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ... and DNA repair. Black arrows indicate natural cellular processes. BER = base excision repair. Note: hypoxanthine...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...adenine deaminase TadA.   After seven rounds of molecular evolution, they obtained four adenine base editors...Cas9 (Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of...10.1038/s41587-020-0491-6 Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R.,...T. P., Raguram, A., Banskota, S., Newby, G. A., Tolar, J., Osborn, M. J., & Liu, D. R. (2023). Evolution...

  12. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... pCas/pTargetF system has been one of the most popular genomic editing systems in E. coli. However, some... linearized pEcgRNA backbone can be prepared in large amounts and frozen for future use or directly ligated...Academy of Sciences (CAS) Center for Excellence in Molecular Plant Sciences in 2019. She joined Sichuan Normal... Yang Lab of the CAS Center for Excellence in Molecular Plant Sciences since December 2020. His research... Yang Lab of the CAS Center for Excellence in Molecular Plant Sciences in September 2019 as a Ph.D. candidate...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...complex approaches to scientific questions become popular. There are only so many methods and machines one...established partnership. If you’re willing to do similar things for your team or lab, you’re likely to be... talking to them at a seminars, or even set up regular meetings. Meeting people outside your organization...I find it useful to remind myself to check in regularly with collaborators for example on a holiday or...regard to reaching out to new people, checking in regularly, and maintaining useful notes that remind me about...

  14. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...chalk talk is effectively a million dollar sales pitch. Given this large sum, one might expect that applicants...accept you as a colleague and give you a million dollar startup package. There are three steps to chalk...resources required to feasibly accomplish the aims and clarify what type of aim you are describing. It's easy ...Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...from purified recombinant proteins at small and large scale.  This new technique involves selection in...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...affinity purification required! The team used other molecular techniques to introduce time modulation of the... New from the viral service By Ina Ersing We regularly add new viral aliquots from our plasmid collection...PHP.V1 that exhibits efficient transduction of vesicular brain cells. Find this AAV at Addgene New serotypes...

  16. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination ...silver staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid...lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a...virus protocols. Browse our plasmid collection of popular lentiviral vectors. This article was written by...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ..., which is not well protected by Cas9. This is similar to the proposed mechanism for engineered pegRNAs...related voltage indicator Ace2N-mNeon also led to similar improvements in performance.      Figure...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...

  18. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...out.  In some fields, a cover letter is vital, particularly in fields where communication and writing are...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover...working for that organization or group. This is particularly important for labs and nonprofits, who tend ... did, and state that it would help you manage a large number of files as part of the job. Structure of.... It can also convey your excitement for that particular job, your commitment to the organization's mission...

  19. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... are just 2 examples of organizations that hold large, annual conferences. Gordon conferences offer more...young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face... Career Expo and the ACS career fair, which are larger meetings offering resources for students seeking...getting a phone interview! If you don’t have a particular employer in mind, you may consider starting with...pages. Make yourself visible on LinkedIn and other popular job site webpages. Importantly, find a good mentor...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work well with a given...1990s, Gateway cloning technology has become very popular as a rapid and highly efficient way to move DNA...has ready-made entry clones available for many popular genes, including Hs.KRAS4a. Use our website to ...Addgene’s ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables...Diagnostic Restriction Digest Video Check Out Our Molecular Biology Reference Pages  ...
Showing: 341 - 360 of 811 results