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Showing: 341 - 360 of 619 results

  2. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...Build: Jennifer created a mobile camera area that included iPad and her laptop raised on an ironing board... the camera, to control some basic functions, including starting and stopping recording).  Test: It was...

  3. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...ideal model organism to study an array of topics including development, genetics, and the nervous system.... an array of biological systems and processes including the nervous system, development, cell signaling...

  6. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...attached to the active site of key metabolic enzymes including biotin carboxylase and decarboxylases. These enzymes...oligonucleotides can be used in a multitude of applications including but not limited to in situ hybridization, blotting...

  7. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...While the biology behind this AAV effect remains unclear, it is likely not a coincidence that the AAV genome...past fifteen years. Some examples from their lab include using AAV to introduce epitope tags into the endogenous...

  9. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...if some of your work does relate, make sure to include that material, but don’t force it. You can always... Having answer slides ready after your formal conclusion slide will also help with your confidence. It...

  10. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications,...pSer-incorporated proteins on a single plasmid. This includes 4 copies of a tRNA that specifically recognizes...

  11. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... search suggests there are hundreds. This only includes general lab strains designed for subcloning or...or protein expression. If you were to include customized strains, the number is probably in the thousands...

  12. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...plasmid sequencing services to verify the plasmids, including long-read sequencing techniques, such as Oxford... presence of a multimer in your sample remains unclear after visualizing it on a gel, one of the other...

  13. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... variants that are less sensitive to ethanol, including the “middle” ADH1 promoter pADH1m, which maintains...3 in a single modular vector for ease of use! Conclusion This post contains only a brief overview of some...

  15. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...with minimal cross-reactivity. Other strategies include using engineered ligases to attach a ligand to ...choosing a dye or ligand to use for your experiment. Conclusion Researchers and vendors continue to develop new...

  17. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...in yeast, plant, insect, and mammalian cells, including human cells. piggyBac is biased to integrate at...all been used to generate transgenic animals, including zebrafish, mice, rats, and rabbits (Sandoval-Villegas...

  18. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... still valuable for anyone in the lab though, including undergrads. I found a lot of career resources ...in microbes until the end of my second year! Conclusion and other pieces of advice Give yourself permission...

  19. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Cre recombinase contained within the knock-in. Including Cre-mediated marker excision, it takes approximately...that recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for...

  20. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...stop and plan it out. Some questions to answer include: What is the goal of the experiment? What are...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...
Showing: 341 - 360 of 619 results