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We narrowed to 384 results for: plasmid dna

Showing: 341 - 360 of 384 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...fusion proteins are made by connecting its DNA code to the cDNA encoding for another protein. Effectively...cells, but not other biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...Novel techniques for the prevention of mitochondrial DNA disorders.” This document is about the popular technique...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Check out our genome engineering guide...scientific possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory...

  3. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...transducing primary cells. What if, instead of supplying a DNA construct, they could simply induce cells to take...10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters...

  4. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...single-stranded) DNA. As the temperature drops, single intensity should smoothly increase as the DNA amplicon ... are produced by the presence of double-stranded DNA, when temperatures are high, signal intensity should...dyes that are not dependent on amplification of the DNA; they are, however, affected by bubbles in wells,...LightCycler qPCR optimisation for low copy number target DNA. Journal of Immunological Methods 270 (2002) 119 ...is a smooth, single-step transition between ssDNA and dsDNA. This is not true for all amplicons; if your... probes (which you probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are... Yes Yes Agarose gel or uMelt analysis dsDNA dye (Sybr)  High No No No Melt curve ...

  6. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo...substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers must...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...creates the DNA double-strand break (DSB) at the target site, and a donor oligonucleotide or plasmid as the... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting..., the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected...provided (in the form of an oligonucleotide or plasmid), precise changes can be made in the genome via...mice carrying the intended mutation. With a donor plasmid, the outcome is less predictable. In our best case...

  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... be used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies... target protein. However, small mutations in the DNA of the hybridoma can occur, known as genetic drift...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...protein (Wang et al., 2013). These proteins are also plasmid-derived. However, a disadvantage to ScFv is their...nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common internal control ... as well as tools for developing and producing plasmid-based antibodies - and we’re always growing our...

  9. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the next...

  10. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... FRET-based biosensors, and interactions between DNA and proteins. FRET, in theory, is an easy technique...used pairs to measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent... fusion protein with your gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan...

  11. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target cells' genomes... ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out Our Cancer Resources Page...

  12. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...self-complementing structure (scAAV) (7). As single stranded DNA viruses, AAVs normally require second strand synthesis... promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001): 1248. PubMed...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection. Cell. Mol. Life...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...

  15. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...essential-but-unlauded role in the identification of DNA’s double helix. Perhaps to the dismay of middle-aged...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up in this...

  16. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member of the Quality...Quality Control team, I help make sure the plasmids that go out our doors are correct. Now that I am heading...

  18. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...use the chromatin IP (ChIP) technique to identify DNA binding proteins involved in histone modification...antibody posts  Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene ...

  19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...expensive) synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly... Generate Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent...

  20. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ... non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the...on Addgene.org Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries...fail to account for the effects of Cas9-induced dsDNA breaks. Are off-target cutting patterns in genome-wide...function, i.e. sites that lack open chromatin marks, DNase hypersensitivity, or are in an enhancer, transcription...control for the effects of guide expression and dsDNA breaks. Figure 2: Non-targeting vs. safe-targeting...for the inherent effects, or background noise, of dsDNA breaks caused by CRISPR. See supplemental figure...
Showing: 341 - 360 of 384 results