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We narrowed to 847 results for: ple

Showing: 341 - 360 of 847 results

  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... requires extensive sample processing to avoid disrupting ribosome-mRNA complexes. Recent work has adapted...time. Both are helping researchers explore the complexity of translational control in cellular physiology...resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat protein binding...constant tracking. You can then directly monitor multiple rounds of translation from this single mRNA; you...cases, creating a reporter mRNA requires inserting multiple hairpin sequences into the coding sequence and...

  2. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...which "profound" seems like an understatement. Multiple speakers from top minds in the stem cell field...just 8 years after the OSKM paper – as a prime example of the fast movement in this field.  As an Addgene...the details. The speakers at this meeting shared plenty of details, but they also uniformly stepped back...uncle with Parkinson's, a half-brother who is a quadriplegic due to a spinal cord injury, and numerous family...expensive Dr. Yamanaka explained that it took 50 people nearly 2 years to create the first stem cell line...not a rate-limiting step in stem cell research. People told me they couldn't have done their work without...

  3. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...particular reference sequence if necessary. The example plasmid on the right has a total size of 7.3kb,.... Avoid star activity. Some endonucleases (for example BamHI) are capable of cleaving sequences which ... The glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a ...you to interpret the bands that you get in your sample lanes. Choose your ladder based on the expected...approximate the DNA mass of comparably intense samples of similar size. Run the gel at 80-150V until you...is not just necessary but also an easy process. Please vist Addgene's resource for plasmid verification...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...connections between different brain regions. For example, communication between the hippocampus and the ...dendrites of proximal neuronal cells. In the specific example of episodic memory, a subset of hippocampal neurons...for review see Dietzschold et al., 2008). Herpes Simplex Virus (HSV) also migrates through neurons where...that express Cre in only specific cell types, for example only dopaminergic neurons, neuronal tracing has...unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with AAV vectors is the Allen...part of the brain in a less invasive manner . For example, in neuromuscular diseases it would be desirable...Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection versus Intradiaphragmatic Injection...

  5. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...motivations, strengths, and learning styles. For example, during my time as a lab scientist, I worked with... management. Invest time in understanding key principles through books, workshops, and online courses ...foster a cohesive, productive environment. For example, in my current role managing a cross-functional...facilitated a team meeting to highlight their complementary strengths. By encouraging alignment on goals... while changing industries and positions. Some people discover they truly enjoy management and choose...successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular biology and metabolism...

  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ... cell research has prompted the development of multiple virus packaging methods. Across these methods,...transfection step of the viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging... to enter the nucleus through the nuclear pore complex. This feature may be useful for the transduction... the components found within the backbone. For example: pLXSN has the wild type LTR, an MCS for cloning...sequence. This post was written and updated by multiple Addgenies. It was originally published in 2018...  Multiple Authors...

  7. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...achievement, the complexity of the device increases, as does the price. With this increased complexity comes the...very difficult to adapt the device to one's own samples and limits the use of microscopy in open science...teleporter," since a part developed in Germany, for example, can also be printed quickly in Madagascar. Platforms...a move towards more open research. The EU, for example, is calling for the opening of sources within the...hardware.    Conclusion Addgene is a great example of how sharing of data and molecular tools can ...

  8. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ... range from a defined pathway or reaction to a complex event such as cancer or even aging. For both a ...differences in these approaches can be found. As an example, say you are considering either a cellular screen...libraries targeting all known genes. This is a perfect example of an unbiased, reverse screen – all known genes...possessing a specific mutation or phenotype. For example, a drug screen may be performed on a cell line ...patients may respond to drug treatment. Another example is using a mouse model of a particular disease ...that do not affect the function of interest. An example where a selection would work is if you are trying...

  9. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.  Listen to the hot plasmids episode...to physically set up in the lab, RNAi is often completely ineffective or results in low penetrant phenotypes...low-cost, cell-free detection assay that can be completed without special equipment. What’s more, detection...encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes with LacZ𝜔 and forms an active LacZ tetramer....red-b-D-galactopyranoside on the assay paper turning it purple, allowing for easy visual detection of the presence...modifying the sequence of the toehold switch. For example, Green has also developed toehold switches to detect...

  10. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...you want to buy, it is worthwhile to test-drive a couple of candidates. Before you buy a new microscope,...microscope, it is smart to look at (and through) a couple of models. Before you start a new project with fluorescent...fluorescent proteins, the best advice is to try a couple of promising variants to check how they perform under...that are related to the imaging strategy and the sample. Practical brightness A protein with a high intrinsic...brightness. Consequently, it makes sense to identify a couple of promising fluorescent proteins and to determine... future application. In figure 2 we provide an example of the comparison of the practical brightness of...

  11. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...cancer precision medicine seems so simple! Take a patient’s tumor sample, use cutting edge genomic technologies...would be extremely helpful as a complementary approach. Also, in principle, these experiments could find...cancer phenotypes. These assays were designed to complement the one assay per gene approach that has been...genes. These should be used to create ever more complete “look-up-tables” of mutant function that could...

  12. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...working to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched...easily be engineered. Like humans, mice express multiple subclasses of IgG antibody. Broad range secondary...subclass specific secondary antibodies to detect multiple targets with different mAbs in the same experiment...secondary antibodies and subclassed switched R-mAbs, multiple targets could now be visualized in a single experiment...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...

  13. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ...This second plasmid saves you a couple of weeks. CRISPR saves you a couple of months. It’s higher throughput...system work? Mendenhall: The system is really quite simple but it offers improvements in efficiency and throughput...right thing to do to put these out there. It’s a simple solution. It would be silly not to share it as ...Since the Addgene page went up last month, over 340 people have signed up to receive alerts when plasmids ...additional tips, tricks, or words of advice for people who may want to apply this tagging system to their...

  14. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...antibodies present throughout our bodies carry out plenty of roles just the way they are, the research antibodies... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...Fc-binding domain (Figure 1).  Figure 1: Examples of sites on antibodies used for conjugation. 1...from antibody to antibody or batch to batch. For example, take lysine conjugation, which is commonly used...reaction or the functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer ...

  15. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...   Fig. 1: A sample containing a polyhistidine tagged protein of interest (purple) and several contaminating...strategy a single protein can be isolated from a complex mix using affinity chromatography against an amino...metal ions are immobilized on a bead or resin and a sample containing the protein of interest flowed through...Ni2+-containing resin. The protein of interest (purple) binds to the Ni2+ and is immobilized along with...tags are also recommended if you need a very pure sample. Because binding strength is proportional to the...

  16. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...fluorescent proteins can be directly compared. An example of a photostability measurement of various cyan...results in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay...intensity decay of a fluorophore does not follow a simple mono-exponential decay (Shaner et al, 2008; Bindels...applications in which far less power is used. For example you might find that you can get useful data from... diffusion, the proteins are (i) trapped in microdroplets in a water/oil emulsion, (ii) embedded in a ...fluorescent protein. In figure 1 we provide an example of such a comparison between cyan fluorescent proteins...

  17. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...listen quietly as people discuss their experiences. If you’re hearing multiple people with similar misconceptions... that a lot of science communication is helping people understand new concepts. You hopefully are aware...necessary” detail after “necessary” detail and end up completely overwhelming your audience - like the professor...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...

  18. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...toxins are the clearest example of a plasmid benefiting its host. For example, cells with a penicillin...Shareck et al., 2004). Plasmid categorization is complex Plasmid classification has shifted over the years...Plasmids may also have more than one function: for example, Gateway cloning relies on genes found on an E....plasmids without clear analogues in nature. For example, helper plasmids were developed to replace helper... Multiple Authors...

  19. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ... features of the gene intact. Here, we show an example gene targeted for tagging with GFP at the C terminus...interest. For a C-terminal tag, as shown in the example above, we inserted a linker and GFP tag at the ...the GFP tag precisely where we need it, in this example, at the C-terminus of the gene of interest. To ...CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor plasmid into the cells we use electroporation... Institute for Cell Science Plasmid Collection, please visit the Cell Catalog on the Allen Cell Explorer...hiPS cell lines generated using these plasmids, please visit the Cell Catalog on the Allen Cell Explorer...

  20. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ... parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that have...genome, they are not commonly used to introduce multiple mutations. This is because as more mutations are...challenge that may arise when crossing mice with multiple mutations is if the mutations are on the same ...expressing strains Cre-lox strains are a common example of mice that are derived from crosses. In Part ...With this two-part guide to mouse modeling now complete, you should be prepared to plan an experimental...
Showing: 341 - 360 of 847 results